scholarly journals Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe

ACS Nano ◽  
2017 ◽  
Vol 11 (12) ◽  
pp. 12632-12640 ◽  
Author(s):  
J. Charles G. Jeynes ◽  
Kalotina Geraki ◽  
Christopher Jeynes ◽  
Mi Zhaohong ◽  
Andrew A. Bettiol ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Super Resolution ◽  
Dual Purpose ◽  
X Ray ◽  
Super Resolution Microscopy

scholarly journals All-Optical Imaging of Gold Nanoparticle Geometry Using Super-Resolution Microscopy

2018 ◽  
Vol 122 (4) ◽  
pp. 2336-2342 ◽  
Author(s):  
Adam Taylor ◽  
René Verhoef ◽  
Michael Beuwer ◽  
Yuyang Wang ◽  
Peter Zijlstra
Keyword(s):  
Optical Imaging ◽  
Gold Nanoparticle ◽  
Super Resolution ◽  
All Optical ◽  
Super Resolution Microscopy

scholarly journals Theranostics with radiation-induced ultrasound emission (TRUE)

2018 ◽  
Vol 11 (03) ◽  
pp. 1830002
Author(s):  
Elijah Robertson ◽  
Liangzhong Xiang
Keyword(s):  
Radiation Dosimetry ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Diffraction ◽  
Cellular Structures ◽  
Fluorescence Labeling ◽  
X Ray ◽  
Radiation Induced ◽  
Ray Projection ◽  
Super Resolution Microscopy

Two novel ultrasound imaging techniques with imaging contrast mechanisms are in the works: X-ray-induced acoustic computed tomography (XACT), and nanoscale photoacoustic tomography (nPAT). XACT has incredible potential in: (1) biomedical imaging, through which a 3D image can be generated using only a single X-ray projection, and (2) radiation dosimetry. nPAT as a new alternative of super-resolution microscopy can break through the optical diffraction limit and is capable of exploring sub-cellular structures without reliance on fluorescence labeling. We expect these new imaging techniques to find widespread applications in both pre-clinical and clinical biomedical research.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

Novel organic dyes for multicolor localization-based super-resolution microscopy

2015 ◽  
Vol 9 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Martin Lehmann ◽  
Gregor Lichtner ◽  
Haider Klenz ◽  
Jan Schmoranzer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Characterization of a Pixelated Cadmium Telluride Detector System Using a Polychromatic X-Ray Source and Gold Nanoparticle-Loaded Phantoms for Benchtop X-Ray Fluorescence Imaging

IEEE Access ◽  
2021 ◽  
Vol 9 ◽  
pp. 49912-49919
Author(s):  
Sandun Jayarathna ◽  
Md Foiez Ahmed ◽  
Liam O'ryan ◽  
Hem Moktan ◽  
Yonggang Cui ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Fluorescence Imaging ◽  
Cadmium Telluride ◽  
Detector System ◽  
X Ray

scholarly journals Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grußmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Fluorogenic Probes ◽  
Super Resolution Microscopy
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