Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

ACS Nano ◽  
2017 ◽  
Vol 11 (5) ◽  
pp. 4926-4933 ◽  
Author(s):  
Ziyi Cheng ◽  
Namhyun Choi ◽  
Rui Wang ◽  
Sangyeop Lee ◽  
Kyung Chul Moon ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Scattering ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Accurate Diagnosis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Specific Antigens ◽  
Prostate Specific Antigens

scholarly journals Duplex Surface Enhanced Raman Scattering-Based Lateral Flow Immunosensor for the Low-Level Detection of Antibiotic Residues in Milk

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5249
Author(s):  
Ruiqi Fan ◽  
Shusheng Tang ◽  
Sunlin Luo ◽  
Hu Liu ◽  
Wanjun Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Lateral Flow ◽  
Antibiotic Residues ◽  
Relative Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Analytical Approaches

A duplex surface enhanced Raman scattering (SERS)-based lateral flow immunosensor was established for the simultaneous detection of two common antibiotic residues including tetracycline and penicillin in milk. The newly synthesized Au@Ag nanoparticles were labeled with different Raman molecules including 5,5-dithiobis-2-nitrobenzoic acid (DTNB) or 4-mercaptobenzoic acid (MBA), followed by the conjugation of anti-tetracycline monoclonal antibody or anti-penicillin receptor, forming two kinds of SERS nanoprobes. The two nanoprobes can recognize tetracycline-BSA and ampicillin-BSA, respectively, which facilitates the simultaneous detection of the two types of antibiotics on a single test line. After optimization, detection limits of tetracycline and penicillin as low as 0.015 ng/mL and 0.010 ng/mL, respectively, were achieved. These values were far below those of most of other documented bio-analytical approaches. Moreover, the spiking test demonstrates an excellent assay accuracy with recoveries of 88.8% to 111.3%, and satisfactory assay precision with relative standard deviation below 16%. Consequently, the results demonstrate that the SERS-based lateral flow immunosensor developed in this study has the advantages of excellent assay sensitivity and remarkable multiplexing capability, thus it will have great application potential in food safety monitoring.

scholarly journals Recent Advances Towards Point-Of-Care Applications of Surface-Enhanced Raman Scattering Sensing

2021 ◽  
Vol 9 ◽  
Author(s):  
Steven Quarin ◽  
Pietro Strobbia
Keyword(s):  
Raman Scattering ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Diagnostic Methods ◽  
Current Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Sensing

The ability to accurately diagnose at the point of care is crucial in many pathologies. However, current standard diagnostic practices can only be performed in specialized health or laboratory settings. To move diagnostic methods from a specialized lab to the point of care many alternate methods have been developed and proposed. Among them surface-enhanced Raman scattering (SERS) sensing offers advantageous features, such as simultaneous detection of multiple biotargets and increased accuracy. Many groups have been working towards the translation of SERS sensing methods from the lab to the point of need. In this mini review, we discuss interesting and recent developments in this effort, focusing on how different sensing mechanism can be used in point-of-care testing applications of SERS.

Simultaneous detection of clenbuterol and ractopamine based on multiplexed competitive surface enhanced Raman scattering (SERS) immunoassay

2017 ◽  
Vol 41 (18) ◽  
pp. 10407-10414 ◽  
Author(s):  
Meng Yu ◽  
Yongjun Hu ◽  
Jianzhi Liu
Keyword(s):  
Raman Scattering ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Multiplexed Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

In this work, we demonstrate a competitive surface-enhanced Raman scattering (SERS) immunoassay for multiplexed detection of clenbuterol and ractopamine.

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