scholarly journals Bacterial Outer Membrane Porins as Electrostatic Nanosieves: Exploring Transport Rules of Small Polar Molecules

ACS Nano ◽  
2017 ◽  
Vol 11 (6) ◽  
pp. 5465-5473 ◽  
Author(s):  
Harsha Bajaj ◽  
Silvia Acosta Gutierrez ◽  
Igor Bodrenko ◽  
Giuliano Malloci ◽  
Mariano Andrea Scorciapino ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Polar Molecules ◽  
Outer Membrane Porins ◽  
Bacterial Outer Membrane

Role of lysines in ion selectivity of bacterial outer membrane porins

1986 ◽  
Vol 860 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Robert E.W. Hancock ◽  
Angela Schmidt ◽  
Katherina Bauer ◽  
Roland Benz
Keyword(s):  
Outer Membrane ◽  
Ion Selectivity ◽  
Outer Membrane Porins ◽  
Bacterial Outer Membrane

Development of the computational antibiotic screening platform (CLASP) to aid in the discovery of new antibiotics

Soft Matter ◽  
2021 ◽  
Author(s):  
Yinghui Dai ◽  
Huilin Ma ◽  
Meishan Wu ◽  
Tory Alane Welsch ◽  
Soor Rajiv Vora ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Potential Drug ◽  
Clock Time ◽  
Output Data ◽  
Drug Molecules ◽  
Outer Membrane Porins ◽  
Antibiotic Screening ◽  
New Antibiotics ◽  
Bacterial Outer Membrane ◽  
Screening Platform

The CLASP is a freely-distributed script for screening potential drug molecules through bacterial outer membrane porins. The automated scripts provide comprehensive thermodynamic and kinetic output data within a few hours of wall-clock time.

scholarly journals Bacterial Outer Membrane Vesicles as Nano‐Scale Bioreactors: A Fatty Acid Conversion Case Study

ChemCatChem ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4080-4086
Author(s):  
Ji‐Won Song ◽  
Yoonjin Baeg ◽  
Ha‐Yeon Jeong ◽  
Jinwon Lee ◽  
Deok‐Kun Oh ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Nano Scale ◽  
Bacterial Outer Membrane

scholarly journals Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 414 ◽  
Author(s):  
Justin Nice ◽  
Nataliya Balashova ◽  
Scott Kachlany ◽  
Evan Koufos ◽  
Eric Krueger ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Aggressive Periodontitis ◽  
Outer Membrane Vesicles ◽  
Host Cells ◽  
Type I ◽  
Outer Membranes ◽  
Independent Mechanism ◽  
Bacterial Outer Membrane

The Gram-negative bacterium, Aggregatibacter actinomycetemcomitans, has been associated with localized aggressive periodontitis (LAP). In particular, highly leukotoxic strains of A. actinomycetemcomitans have been more closely associated with this disease, suggesting that LtxA is a key virulence factor for A. actinomycetemcomitans. LtxA is secreted across both the inner and outer membranes via the Type I secretion system, but has also been found to be enriched within outer membrane vesicles (OMVs), derived from the bacterial outer membrane. We have characterized the association of LtxA with OMVs produced by the highly leukotoxic strain, JP2, and investigated the interaction of these OMVs with host cells to understand how LtxA is delivered to host cells in this OMV-associated form. Our results demonstrated that a significant fraction of the secreted LtxA exists in an OMV-associated form. Furthermore, we have discovered that in this OMV-associated form, the toxin is trafficked to host cells by a cholesterol- and receptor-independent mechanism in contrast to the mechanism by which free LtxA is delivered. Because OMV-associated toxin is trafficked to host cells in an entirely different manner than free toxin, this study highlights the importance of studying both free and OMV-associated forms of LtxA to understand A. actinomycetemcomitans virulence.

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