scholarly journals Morphological Evolution of Electrochemically Plated/Stripped Lithium Microstructures Investigated by Synchrotron X-ray Phase Contrast Tomography

ACS Nano ◽  
2016 ◽  
Vol 10 (8) ◽  
pp. 7990-7997 ◽  
Author(s):  
Fu Sun ◽  
Lukas Zielke ◽  
Henning Markötter ◽  
André Hilger ◽  
Dong Zhou ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Morphological Evolution ◽  
X Ray ◽  
Phase Contrast Tomography

Investigation of Herculaneum Papyri by X-Ray Phase-Contrast Tomography

2019 ◽  
pp. 299-324
Author(s):  
Inna Bukreeva ◽  
Graziano Ranocchia ◽  
Vincenzo Formoso ◽  
Michele Alessandrelli ◽  
Michela Fratini ◽  
...  
Keyword(s):  
Phase Contrast ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

2018 ◽  
Vol 115 (27) ◽  
pp. 6940-6945 ◽  
Author(s):  
Mareike Töpperwien ◽  
Franziska van der Meer ◽  
Christine Stadelmann ◽  
Tim Salditt
Keyword(s):  
Phase Contrast ◽  
Spatial Organization ◽  
Granular Layer ◽  
Three Dimensional ◽  
Cell Segmentation ◽  
X Ray ◽  
Human Cerebellum ◽  
Conventional Histology ◽  
3D Information ◽  
Phase Contrast Tomography

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human cerebellum embedded in paraffin and show that isotropic subcellular resolution can be reached at both setups throughout the specimen. To enable a quantitative analysis of the reconstructed data, we demonstrate automatic cell segmentation and localization of over 1 million neurons within the cerebellar cortex. This allows for the analysis of the spatial organization and correlation of cells in all dimensions by borrowing concepts from condensed-matter physics, indicating a strong short-range order and local clustering of the cells in the granular layer. By quantification of 3D neuronal “packing,” we can hence shed light on how the human cerebellum accommodates 80% of the total neurons in the brain in only 10% of its volume. In addition, we show that the distribution of neighboring neurons in the granular layer is anisotropic with respect to the Purkinje cell dendrites.

scholarly journals 3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?

2020 ◽  
Vol 55 (10) ◽  
pp. 1261-1267
Author(s):  
Niccolò Peruzzi ◽  
Béla Veress ◽  
Lars B. Dahlin ◽  
Tim Salditt ◽  
Mariam Andersson ◽  
...  
Keyword(s):  
Myenteric Plexus ◽  
Phase Contrast ◽  
3D Analysis ◽  
X Ray ◽  
Phase Contrast Tomography

Investigation of inner ear anatomy in mouse using X‐ray phase contrast tomography

2019 ◽  
Vol 82 (7) ◽  
pp. 953-960
Author(s):  
Hong‐Xia Yin ◽  
Peng Zhang ◽  
Zheng Wang ◽  
Yun‐Fu Liu ◽  
Ying Liu ◽  
...  
Keyword(s):  
Inner Ear ◽  
Phase Contrast ◽  
X Ray ◽  
Phase Contrast Tomography
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