scholarly journals A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation

ACS Nano ◽  
2016 ◽  
Vol 10 (9) ◽  
pp. 8346-8354 ◽  
Author(s):  
Emily Elizabeth Louise Lewis ◽  
Helen Wheadon ◽  
Natasha Lewis ◽  
Jingli Yang ◽  
Margaret Mullin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Magnetic Levitation ◽  
Bone Marrow Niche ◽  
Niche Model

Proteomic Profiling Identifies Distinct Bone Marrow Niche Mesenchymal Stem Cell Populations In AML Patients

2015 ◽  
Vol 15 ◽  
pp. S19
Author(s):  
Peter P. Ruvolo ◽  
Rui-Yu Wang ◽  
Vivian Ruvolo ◽  
Zhihong Zeng ◽  
YiHua Qiu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Populations ◽  
Bone Marrow Niche ◽  
Proteomic Profiling ◽  
Stem Cell Populations

scholarly journals Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence

2017 ◽  
Vol 8 ◽  
pp. 204173141770442 ◽  
Author(s):  
Natasha S Lewis ◽  
Emily EL Lewis ◽  
Margaret Mullin ◽  
Helen Wheadon ◽  
Matthew J Dalby ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Magnetic Nanoparticles ◽  
Mesenchymal Stem Cell ◽  
Collagen Gel ◽  
Cell Phenotype ◽  
Type I ◽  
Bone Marrow Niche ◽  
Multicellular Spheroids ◽  
Cell Spheroids

Multicellular spheroids are an established system for three-dimensional cell culture. Spheroids are typically generated using hanging drop or non-adherent culture; however, an emerging technique is to use magnetic levitation. Herein, mesenchymal stem cell spheroids were generated using magnetic nanoparticles and subsequently cultured within a type I collagen gel, with a view towards developing a bone marrow niche environment. Cells were loaded with magnetic nanoparticles, and suspended beneath an external magnet, inducing self-assembly of multicellular spheroids. Cells in spheroids were viable and compared to corresponding monolayer controls, maintained stem cell phenotype and were quiescent. Interestingly, core spheroid necrosis was not observed, even with increasing spheroid size, in contrast to other commonly used spheroid systems. This mesenchymal stem cell spheroid culture presents a potential platform for modelling in vitro bone marrow stem cell niches, elucidating interactions between cells, as well as a useful model for drug delivery studies.

scholarly journals A novel 3D mesenchymal stem cell model of the multiple myeloma bone marrow niche: biologic and clinical applications

Oncotarget ◽  
2016 ◽  
Vol 7 (47) ◽  
pp. 77326-77341 ◽  
Author(s):  
Jana Jakubikova ◽  
Danka Cholujova ◽  
Teru Hideshima ◽  
Paulina Gronesova ◽  
Andrea Soltysova ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Model ◽  
Clinical Applications ◽  
Bone Marrow Niche ◽  
Stem Cell Model

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

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