Photoactivated Bacteriorhodopsin/SiNx Nanopore-Based Biological Nanofluidic Generator with Single-Protein Sensitivity

ACS Nano ◽  
2022 ◽  
Author(s):  
Dandan Wang ◽  
Yong Wang ◽  
Haijuan Li ◽  
Yanchao Han ◽  
Ping Hu ◽  
...  
Keyword(s):  
Single Protein

Orientation of actin filaments during motion in motility assay

1995 ◽  
Vol 53 ◽  
pp. 52-53
Author(s):  
J. Borejdo ◽  
S. Burlacu
Keyword(s):  
Actin Filaments ◽  
Thin Filament ◽  
Striated Muscle ◽  
Myosin Head ◽  
Classical Method ◽  
Polarization Of Fluorescence ◽  
Single Protein ◽  
Intracellular Organelles ◽  
Change Orientation ◽  
Active Entity

Polarization of fluorescence is a classical method to assess orientation or mobility of macromolecules. It has been a common practice to measure polarization of fluorescence through a microscope to characterize orientation or mobility of intracellular organelles, for example anisotropic bands in striated muscle. Recently, we have extended this technique to characterize single protein molecules. The scientific question concerned the current problem in muscle motility: whether myosin heads or actin filaments change orientation during contraction. The classical view is that the force-generating step in muscle is caused by change in orientation of myosin head (subfragment-1 or SI) relative to the axis of thin filament. The molecular impeller which causes this change resides at the interface between actin and SI, but it is not clear whether only the myosin head or both SI and actin change orientation during contraction. Most studies assume that observed orientational change in myosin head is a reflection of the fact that myosin is an active entity and actin serves merely as a passive "rail" on which myosin moves.

scholarly journals Human oxygenase variants employing a single protein Fe(II) ligand are catalytically active

2021 ◽  
Author(s):  
Amelia Brasnett ◽  
Inga Pfeffer ◽  
Lennart Brewitz ◽  
Rasheduzzaman Chowdhury ◽  
Yu Nakashima ◽  
...  
Keyword(s):  
Single Protein ◽  
Catalytically Active

scholarly journals Human oxygenase variants employing a single protein Fe(II) ligand are catalytically active

2021 ◽  
Author(s):  
Amelia Brasnett ◽  
Inga Pfeffer ◽  
Lennart Brewitz ◽  
Rasheduzzaman Chowdhury ◽  
Yu Nakashima ◽  
...  
Keyword(s):  
Single Protein ◽  
Catalytically Active

Anatomie, histologie et affinités de l'appareil séricigène des Hersilia Sav. et Aud. (Araneae: Hersiliidae)

1984 ◽  
Vol 62 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Jacqueline Kovoor
Keyword(s):  
Type A ◽  
The Other ◽  
Secretory Cells ◽  
Evolutionary Stage ◽  
Type B ◽  
Outer Coat ◽  
Silk Glands ◽  
Single Protein

Although hersiliid spiders do not spin any webs, their silk glands, which belong to six types, are large and complex. Two groups of ampullate glands, one opening on the anterior spinnerets and the other on the median spinnerets, secrete two proteins each. About 180 pyriform glands are clearly bipartite. Over 200 type A aciniform glands opening on the median and posterior spinnerets are made up of three categories of secretory cells. Silk from these glands consists of two proteins (core and outer coat) joined together by an intermediary layer of acidic glycoprotein. All the 160 type B aciniform glands opening on the posterior spinnerets secrete a single protein. Fifty tubuliform glands opening on the median and posterior spinnerets produce two proteins, one of which is coloured. As in Urocteinae, long posterior spinnerets and large, numerous aciniform and tubuliform glands are correlated with swathing of prey and egg-cocoon construction. In Lycosidae and Agelenidae, the ampullate glands show the same number and distribution according to the spinnerets. However, anatomical and histochemical features of hersiliid aciniform and ampullate glands are close to those of some Araneoidea. Apart from peculiar characteristics, silk glands of Hersilia might represent an intermediate evolutionary stage towards Araneoidea.

scholarly journals Highly Sensitive Protein Quantification by Direct Kinetic Fingerprinting of Single Protein Molecules

2021 ◽  
Vol 120 (3) ◽  
pp. 185a
Author(s):  
Tanmay Chatterjee ◽  
Achim Knappik ◽  
Erin Sandford ◽  
Muneesh Tewari ◽  
Sung Won Choi ◽  
...  
Keyword(s):  
Protein Quantification ◽  
Highly Sensitive ◽  
Protein Molecules ◽  
Single Protein
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