Biomimetic Hydroxyapatite Nanorods Promote Bone Regeneration via Accelerating Osteogenesis of BMSCs through T Cell-Derived IL-22

ACS Nano ◽  
2022 ◽  
Author(s):  
Fei Yu ◽  
Ruixian Lian ◽  
Lu Liu ◽  
Ting Liu ◽  
Chao Bi ◽  
...  
Keyword(s):  
T Cell ◽  
Bone Regeneration ◽  
Biomimetic Hydroxyapatite

scholarly journals Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration

2019 ◽  
Vol 20 (23) ◽  
pp. 6002 ◽  
Author(s):  
Chih-Hsiang Fang ◽  
Yi-Wen Lin ◽  
Feng-Huei Lin ◽  
Jui-Sheng Sun ◽  
Yuan-Hung Chao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Regeneration ◽  
Stromal Cell ◽  
Alveolar Bone ◽  
Therapeutic Potential ◽  
Bone Defects ◽  
Stromal Cell Derived Factor ◽  
Biomimetic Hydroxyapatite

The development of a novel alloplastic graft with both osteoinductive and osteoconductive properties is still necessary. In this study, we tried to synthesize a biomimetic hydroxyapatite microspheres (gelatin/nano-hydroxyapatite microsphere embedded with stromal cell-derived factor-1: GHM-S) from nanocrystalline hydroxyapatites and to investigate their therapeutic potential and effects on bone regeneration. In this study, hydroxyapatite was synthesized by co-precipitation of calcium hydroxide and orthophosphoric acid to gelatin solution. The microbial transglutaminase was used as the agent to crosslink the microspheres. The morphology, characterization, and thermal gravimetric analysis of microspheres were performed. SDF-1 release profile and in vitro biocompatibility and relative osteogenic gene expression were analyzed, followed by in vivo micro-computed tomography study and histological analysis. The synthesized hydroxyapatite was found to be similar to hydroxyapatite of natural bone tissue. The stromal cell-derived factor-1 was embedded into gelatin/hydroxyapatite microsphere to form the biomimetic hydroxyapatite microsphere. The stromal cell-derived factor-1 protein could be released in a controlled manner from the biomimetic hydroxyapatite microsphere and form a concentration gradient in the culture environment to attract the migration of stem cells. Gene expression and protein expression indicated that stem cells could differentiate or develop into pre-osteoblasts. The effect of bone formation by the biomimetic hydroxyapatite microsphere was assessed by an in vivo rats’ alveolar bone defects model and confirmed by micro-CT imaging and histological examination. Our findings demonstrated that the biomimetic hydroxyapatite microsphere can enhance the alveolar bone regeneration. This design has potential be applied to other bone defects.

scholarly journals Biomimetic hydroxyapatite/poly xylitol sebacic adibate/vitamin K nanocomposite for enhancing bone regeneration

2019 ◽  
Vol 47 (1) ◽  
pp. 1898-1907 ◽  
Author(s):  
Zhipeng Dai ◽  
Minyan Dang ◽  
Wenzhi Zhang ◽  
Sumathra Murugan ◽  
Seoh Wei Teh ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Vitamin K ◽  
Biomimetic Hydroxyapatite

scholarly journals Individual Effector/Regulator T Cell Ratios Impact Bone Regeneration

2019 ◽  
Vol 10 ◽  
Author(s):  
Claudia Schlundt ◽  
Simon Reinke ◽  
Sven Geissler ◽  
Christian H. Bucher ◽  
Carolin Giannini ◽  
...  
Keyword(s):  
T Cell ◽  
Bone Regeneration

scholarly journals BMP4 Moderates Glycolysis and Regulates Activation and Interferon-Gamma Production in CD4+ T Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Feng Huang ◽  
Lei Hu ◽  
Yuanmin Zhang ◽  
Xingmin Qu ◽  
Junji Xu
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Regulatory T Cells ◽  
Bone Regeneration ◽  
Interferon Gamma ◽  
Cd4 T Cells ◽  
T Cell Development ◽  
Ifn Γ

BMP4 is a key growth factor well known in promoting bone regeneration and has been reported to be able to regulate T cell development in the thymus. Here, we showed that BMP4 downregulates the activation of naïve CD4+ T cells and the IFN-γ production of CD4+ T cells without increasing regulatory T cells. BMP4 could also moderate glycolysis of T cells and regulate Hif1α expression. Furthermore, BMP4 showed a suppressive function on the IFN-γ production of CD4+ T cells in vivo. These findings indicating a mechanism by which BMP-4 may regulate activation and IFN-γ production in CD4+ T cells via metabolism moderation and suggests that BMP4 may be a potential therapeutic supplement in autoinflammatory diseases.

scholarly journals Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration

2012 ◽  
Vol 23 (9) ◽  
pp. 2115-2126 ◽  
Author(s):  
Chi-Kai Chiu ◽  
Joao Ferreira ◽  
Tzy-Jiun M. Luo ◽  
Haixia Geng ◽  
Feng-Chang Lin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Cross Linker ◽  
Biomimetic Hydroxyapatite

Electron microscope study of the secretory activity of epithelial cells in embryonic thymus

1990 ◽  
Vol 48 (3) ◽  
pp. 382-383
Author(s):  
H. Alasam
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Epithelial Cells ◽  
Electron Density ◽  
Secretory Activity ◽  
T Cell Differentiation ◽  
High Electron ◽  
Transmission Electron ◽  
Medullary Epithelial Cells ◽  
Thymic Factor

The possibility that intrathymic T-cell differentiation involves stem cell-lymphoid interactions in embryos led us to study the ultrastructure of epithelial cell in normal embryonic thymus. Studies in adult thymus showed that it produces several peptides that induce T-cell differentiation. Several of them have been chemically characterized, such as thymosin α 1, thymopoietin, thymic humoral factor or the serum thymic factor. It was suggested that most of these factors are secreted by populations of A and B-epithelial cells.Embryonic materials were obtained from inbred matings of Swiss Albino mice. Thymuses were disected from embryos 17 days old and prepared for transmission electron microscopy. Our studies showed that embryonic thymus at this stage contains undifferentiated and differentiated epithelial cells, large lymphoblasts, medium and few small lymphocytes (Fig. 5). No differences were found between cortical and medullary epithelial cells, in contrast to the findings of Van Vliet et al,. Epithelial cells were mostly of the A-type with low electron density in both cytoplasm and nucleus. However few B-type with high electron density were also found (Fig. 7).

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

FAILURE TO DETECT ANTI-HTLV-1 ANTIBODY IN A PATIENT WITH ADULT T-CELL LEUKAEMIA/LYMPHOMA

1998 ◽  
Vol 103 (4) ◽  
pp. 1207-1208 ◽  
Author(s):  
Shan-Shun Luo ◽  
Hideto Tamura ◽  
Norio Yokose ◽  
Kiyoyuki Ogata ◽  
Kazuo Dan
Keyword(s):  
T Cell ◽  
Cell Leukaemia
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