Polystyrene Nanoplastics Inhibit the Transformation of Tetrabromobisphenol A by the Bacterium Rhodococcus jostii

ACS Nano ◽  
2021 ◽  
Author(s):  
Shen Xu ◽  
Chao Wu ◽  
Wen-Bo Guo ◽  
Liuyan Yang ◽  
Rong Ji ◽  
...  
Keyword(s):  
Tetrabromobisphenol A ◽  
Rhodococcus Jostii

Transformation of tetrabromobisphenol A by Rhodococcus jostii RHA1: Effects of heavy metals

Chemosphere ◽  
2018 ◽  
Vol 196 ◽  
pp. 206-213 ◽  
Author(s):  
Shen Xu ◽  
Yong-Feng Wang ◽  
Liu-Yan Yang ◽  
Rong Ji ◽  
Ai-Jun Miao
Keyword(s):  
Heavy Metals ◽  
Tetrabromobisphenol A ◽  
Rhodococcus Jostii Rha1 ◽  
Rhodococcus Jostii

The disposition and metabolism of tetrabromobisphenol-A after a single i.p. dose in the rat

Chemosphere ◽  
2001 ◽  
Vol 45 (4-5) ◽  
pp. 693-700 ◽  
Author(s):  
J.A. Szymańska ◽  
A. Sapota ◽  
B. Frydrych
Keyword(s):  
Tetrabromobisphenol A

scholarly journals A Synthetic Biology Approach Using Engineered Bacteria to Detect Perfluoroalkyl Substance (PFAS) Contamination in Water

2021 ◽  
Vol 186 (Supplement_1) ◽  
pp. 801-807
Author(s):  
Nathaniel A Young ◽  
Ryan L Lambert ◽  
Angela M Buch ◽  
Christen L Dahl ◽  
Jackson D Harris ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Dna Sequences ◽  
Detection System ◽  
The United States ◽  
Detection Methods ◽  
Health Concern ◽  
Contaminated Water ◽  
Engineered Bacteria ◽  
Synthetic Biology Approach ◽  
Rhodococcus Jostii

ABSTRACT Introduction Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic compounds used industrially for a wide variety of applications. These PFAS compounds are very stable and persist in the environment. The PFAS contamination is a growing health issue as these compounds have been reported to impact human health and have been detected in both domestic and global water sources. Contaminated water found on military bases poses a potentially serious health concern for active duty military, their families, and the surrounding communities. Previous detection methods for PFAS in contaminated water samples require expensive and time-consuming testing protocols that limit the ability to detect this important global pollutant. The main objective of this work was to develop a novel detection system that utilizes a biological reporter and engineered bacteria as a way to rapidly and efficiently detect PFAS contamination. Materials and Methods The United States Air Force Academy International Genetically Engineered Machine team is genetically engineering Rhodococcus jostii strain RHA1 to contain novel DNA sequences composed of a propane 2-monooxygenase alpha (prmA) promoter and monomeric red fluorescent protein (mRFP). The prmA promoter is activated in the presence of PFAS and transcribes the mRFP reporter. Results The recombinant R. jostii containing the prmA promoter and mRFP reporter respond to exposure of PFAS by activating gene expression of the mRFP. At 100 µM of perfluorooctanoic acid, the mRFP expression was increased 3-fold (qRT-PCR). Rhodococcus jostii without exposure to PFAS compounds had no mRFP expression. Conclusions This novel detection system represents a synthetic biology approach to more efficiently detect PFAS in contaminated samples. With further refinement and modifications, a similar system could be readily deployed in the field around the world to detect this critical pollutant.

Sign in / Sign up

Export Citation Format

Share Document