scholarly journals Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins

ACS Nano ◽  
2021 ◽  
Author(s):  
Joschka Hellmeier ◽  
René Platzer ◽  
Vanessa Mühlgrabner ◽  
Magdalena C. Schneider ◽  
Elke Kurz ◽  
...  
Keyword(s):  
Dna Origami ◽  
Site Specific ◽  
Intact Proteins

Programmable site‐specific functionalization of DNA origami with polynucleotide brushes

2021 ◽  
Author(s):  
Yunqi Yang ◽  
Qinyi Lu ◽  
Chao-Min Huang ◽  
Hongji Qian ◽  
Yunlong Zhang ◽  
...  
Keyword(s):  
Dna Origami ◽  
Site Specific

scholarly journals Programmable site-specific functionalization of DNA origami with polymeric nucleotide brushes

2021 ◽  
Author(s):  
Yunqi Yang ◽  
Qinyi Lu ◽  
Chao-min Huang ◽  
Hongji Qian ◽  
Sonal Deshpande ◽  
...  
Keyword(s):  
Dna Origami ◽  
Site Specific

Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures

2012 ◽  
Vol 124 (10) ◽  
pp. 2471-2474 ◽  
Author(s):  
Eiji Nakata ◽  
Fong Fong Liew ◽  
Chisana Uwatoko ◽  
Shigeki Kiyonaka ◽  
Yasuo Mori ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Specific Protein ◽  
Dna Origami ◽  
Site Specific

Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures

2012 ◽  
Vol 51 (10) ◽  
pp. 2421-2424 ◽  
Author(s):  
Eiji Nakata ◽  
Fong Fong Liew ◽  
Chisana Uwatoko ◽  
Shigeki Kiyonaka ◽  
Yasuo Mori ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Specific Protein ◽  
Dna Origami ◽  
Site Specific

scholarly journals Recent Advances in Novel DNA Guiding Nanofabrication and Nanotechnology

2018 ◽  
Vol 4 (1) ◽  
pp. 32-52 ◽  
Author(s):  
Zhiguang Suo ◽  
Jingqi Chen ◽  
Ziheng Hu ◽  
Yihao Liu ◽  
Feifei Xing ◽  
...  
Keyword(s):  
Dna Recognition ◽  
Genetic Material ◽  
Dna Nanotechnology ◽  
Structural Features ◽  
Dna Origami ◽  
Site Specific ◽  
Metal Organic ◽  
Nanomaterials Synthesis ◽  
Promising Development ◽  
3D Topology

Abstract DNA as life’s genetic material has been widely investigated around the world. In recent years, with the fiery researches on nanomaterials, it also plays an important role in the development of material science due to its extraordinary molecular recognition capability and prominent structural features. In this mini review, we mainly overview the recent progresses of DNA guiding self-assembled nanostructures and nanofabrication. Typical DNA tile-based assembly and DNA origami nanotechnologies are presented, utilizing the recent 3D topology methods to fabricate multidimensional structures with unique properties. Then the site-specific nanomaterials synthesis and nano-DNA recognition on different DNA scaffolds/templates are demonstrated with excellent addressability, biocompatibility and structural programmability. Various nanomaterials, such as metals, carbon family materials, quantum dots, metal-organic frameworks, and DNA-based liquid crystals are briefly summarized. Finally, the present limitation and future promising development directions are discussed in conclusion and perspective. We wish this review would provide useful information toward the broader scientific interests in DNA nanotechnology.

DNA ORIGAMI SITE-SPECIFIC ARRANGEMENT OF GOLD NANOPARTICLES

NANO ◽  
2013 ◽  
Vol 08 (06) ◽  
pp. 1350064
Author(s):  
AMOAKO GEORGE ◽  
RIAN YE ◽  
LIZHOU ZHUANG ◽  
XIAOHONG YANG ◽  
ZHIYONG SHEN ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gel Electrophoresis ◽  
Dna Origami ◽  
Covalent Attachment ◽  
Agarose Gel Electrophoresis ◽  
Dna Oligomers ◽  
Site Specific ◽  
Modified Dna ◽  
Vis Spectroscopy ◽  
Potential Use

Controlling matter at the nanoscale holds a lot of promise in nanotechnology. The DNA origami is promising if used as a template to design and arrange matter at the nanoscale. We have used the DNA origami approach to engineer staple strands at selected sites for attachment of gold nanoparticles. The covalent attachment of thiol-modified DNA oligomers was used to functionalize gold nanoparticles. These oligomers then hybridize with complementary strands extended on selected staple strands on the DNA origami surface with nanometer precision. Gold nanoparticles of 5 nm diameter were arranged across a DNA origami tube to form a C -shape which has potential use in electronics and plasmonics. Agarose gel electrophoresis, AFM, UV-Vis spectroscopy and TEM were used to characterize the structure.

Programmable site‐specific functionalization of DNA origami with polynucleotide brushes

2021 ◽  
Author(s):  
Yunqi Yang ◽  
Qinyi Lu ◽  
Chao-Min Huang ◽  
Hongji Qian ◽  
Yunlong Zhang ◽  
...  
Keyword(s):  
Dna Origami ◽  
Site Specific

scholarly journals Site-Specific Generation of Protein-Protein Conjugates Using Native Amino Acids

2019 ◽  
Author(s):  
Marco Lobba ◽  
Christof Fellmann ◽  
Alan Marmelstein ◽  
Johnathan Maza ◽  
Elijah Kissman ◽  
...  
Keyword(s):  
Amino Acids ◽  
Vaccine Development ◽  
Chimeric Protein ◽  
Cell Penetrating Peptides ◽  
New Paradigm ◽  
Site Specific ◽  
New Approach ◽  
Intact Proteins ◽  
Tyrosine Residues ◽  
Protein Conjugates

<p>Chimeric protein-protein conjugates provide platforms for immunotherapy, targeted drug delivery, and vaccine development. However, many desirable constructs cannot be produced through direct expression, and the targeted coupling of two proteins is chemically challenging. Here we present a new approach for the rapid and site-specific coupling of proteins using native amino acids. Tyrosinase oxidizes exposed tyrosine residues on polypeptides, generating <i>ortho</i>-quinones that react rapidly with strategically placed cysteine residues in other proteins. This approach was used to modify CRISPR-Cas9 and other substrates with small molecules, peptides and even intact proteins. The conjugation of cell penetrating peptides to CRISPR-Cas9 was shown to increase cellular genome editing efficiency by 20-fold relative to unmodified Cas9. This technology represents a new paradigm for biomolecular coupling, and paves the way to an unprecedented range of multifunctional bioconjugates.</p>

scholarly journals Site-Specific Metallization of Multiple Metals on a Single DNA Origami Template

Langmuir ◽  
2014 ◽  
Vol 30 (4) ◽  
pp. 1134-1141 ◽  
Author(s):  
Bibek Uprety ◽  
Elisabeth P. Gates ◽  
Yanli Geng ◽  
Adam T. Woolley ◽  
John N. Harb
Keyword(s):  
Dna Origami ◽  
Site Specific
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