Detection of Single Cancer Cells in Blood with Artificially Intelligent Nanoarray

ACS Nano ◽  
2021 ◽  
Author(s):  
Reef Einoch-Amor ◽  
Yoav Y. Broza ◽  
Hossam Haick
Keyword(s):  
Cancer Cells ◽  
Single Cancer

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

scholarly journals Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell Cycle Exit after DNA Damage

2020 ◽  
Author(s):  
Hendrika A. Segeren ◽  
Lotte van Rijnberk ◽  
Eva Moreno ◽  
Frank Riemers ◽  
Ruixue Yuan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Cancer Cells ◽  
Cell Cycle Exit ◽  
Single Cancer

scholarly journals A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57712 ◽  
Author(s):  
Alejandro San Martín ◽  
Sebastián Ceballo ◽  
Iván Ruminot ◽  
Rodrigo Lerchundi ◽  
Wolf B. Frommer ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Warburg Effect ◽  
Single Cancer ◽  
Lactate Sensor ◽  
The Warburg Effect

Abstract 3527: Combined high-throughput DNA genotyping and protein quantification in single cancer cells

2019 ◽  
Author(s):  
Benjamin Demaree ◽  
Cyrille Delley ◽  
David Ruff ◽  
Sebastian Treusch ◽  
Dennis Eastburn ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Protein Quantification ◽  
Single Cancer ◽  
Dna Genotyping

scholarly journals Analysis of Single Nucleotide-Mutated Single-Cancer Cells Using the Combined Technologies of Single-Cell Microarray Chips and Peptide Nucleic Acid-DNA Probes

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 628
Author(s):  
Hajime Shigeto ◽  
Eriko Yamada ◽  
Mizuki Kitamatsu ◽  
Takashi Ohtsuki ◽  
Akira Iizuka ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Peptide Nucleic Acid ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Cancer Tissue ◽  
Single Nucleotide ◽  
Single Cancer ◽  
Microarray Chips ◽  
Cell Microarray

Research into cancer cells that harbor gene mutations relating to anticancer drug-resistance at the single-cell level has focused on the diagnosis of, or treatment for, cancer. Several methods have been reported for detecting gene-mutated cells within a large number of non-mutated cells; however, target single nucleotide-mutated cells within a large number of cell samples, such as cancer tissue, are still difficult to analyze. In this study, a new system is developed to detect and isolate single-cancer cells expressing the T790M-mutated epidermal growth factor receptor (EGFR) mRNA from multiple non-mutated cancer cells by combining single-cell microarray chips and peptide nucleic acid (PNA)-DNA probes. The single-cell microarray chip is made of polystyrene with 62,410 microchambers (31-40 µm diameter). The T790M-mutated lung cancer cell line, NCI-H1975, and non-mutated lung cancer cell line, A549, were successfully separated into single cells in each microchambers on the chip. Only NCI-H1975 cell was stained on the chip with a fluorescein isothiocyanate (FITC)-conjugated PNA probe for specifically detecting T790M mutation. Of the NCI-H1975 cells that spiked into A549 cells, 0–20% were quantitatively analyzed within 1 h, depending on the spike concentration. Therefore, our system could be useful in analyzing cancer tissue that contains a few anticancer drug-resistant cells.

scholarly journals Electronic Detection of Single Cancer Cells with Graphene Field Effect Transistors

2017 ◽  
Vol 112 (3) ◽  
pp. 461a ◽  
Author(s):  
James Froberg ◽  
Prajakta Kulkarni ◽  
Sanku Mallik ◽  
Yongki Choi
Keyword(s):  
Cancer Cells ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Electronic Detection ◽  
Single Cancer

scholarly journals The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model

Micromachines ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 9 ◽  
Author(s):  
Dettachai Ketpun ◽  
Achariya Sailasuta ◽  
Thammawit Suwannaphan ◽  
Sudchaya Bhanpattanakul ◽  
Alongkorn Pimpin ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cancer Cells ◽  
Tumor Model ◽  
Cell Tumor ◽  
Shear Stresses ◽  
Mast Cell Tumor ◽  
Hydrodynamic Shear ◽  
Single Cancer ◽  
Cutaneous Mast Cell ◽  
Spiral Microchannel

In vivoandin situmonitoring of the nitric oxide stimulus response of single cancer cells by Raman spectroscopy

2013 ◽  
Vol 10 (4) ◽  
pp. 045608 ◽  
Author(s):  
L Su ◽  
Y Chen ◽  
G N Zhang ◽  
L H Wang ◽  
A G Shen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Stimulus Response ◽  
Single Cancer

scholarly journals Quantum cascade laser infrared spectroscopy of single cancer cells

2017 ◽  
Author(s):  
Imran I. Patel ◽  
Vijayakumar P. Rajamanickam ◽  
Andrea Bertoncini ◽  
Francesca Pagliari ◽  
Luca Tirinato ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Cancer Cells ◽  
Quantum Cascade Laser ◽  
Quantum Cascade ◽  
Single Cancer
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