Zika Virus NS5 Forms Supramolecular Nuclear Bodies That Sequester Importin-α and Modulate the Host Immune and Pro-Inflammatory Response in Neuronal Cells

2019 ◽  
Vol 5 (6) ◽  
pp. 932-948 ◽  
Author(s):  
Ivan H. W. Ng ◽  
Kitti Wing-Ki Chan ◽  
Min Jie Alvin Tan ◽  
Chin Piaw Gwee ◽  
Kate M. Smith ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Zika Virus ◽  
Neuronal Cells ◽  
Nuclear Bodies ◽  
Importin Α

The Protective Effect of Picroside II on Isoflurane-Induced Neuronal Injury in Rats via Downregulating miR-195

2021 ◽  
pp. 1-9
Author(s):  
Hui Li ◽  
Weijia Du ◽  
Yawei Yuan ◽  
Jingjing Xue ◽  
Qiang Li ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Protective Effect ◽  
Inflammatory Cytokines ◽  
Escape Latency ◽  
Neuronal Cells ◽  
Neuronal Injury ◽  
Morris Water Maze Test ◽  
Picroside Ii ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

<b><i>Introduction:</i></b> Numerous pieces of evidence demonstrated that isoflurane induces hippocampal cell injury and cognitive impairments. Picroside II has been investigated for its anti-apoptosis and antioxidant neuroprotective effects. We aimed to explore the protective effects of picroside II and the role of microRNA-195 (miR-195) on isoflurane-induced neuronal injury in rats. <b><i>Methods:</i></b> The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding escape latency and time in quadrant parameters. Real-time quantitative PCR was used to detect the expression levels of miR-195 and pro-inflammatory cytokines, including inter­leukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) mRNA, in the hippocampal tissues and neuronal cells. <b><i>Results:</i></b> The picroside II significantly improves isoflurane-induced higher escape latency and lower time spent in the quadrant compared with the control rats. Picroside II also promotes cell viability and suppresses cell apoptosis of isoflurane-induced neuronal cells. Besides, picroside II suppresses the expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and miR-195 in vivo and in vitro. Furthermore, overexpression of miR-195 abrogates the effects of picroside II on the expression of pro-inflammatory cytokines. The appropriate dose of picroside II is 20 mg/kg. <b><i>Conclusion:</i></b> Picroside II could protect the nervous system possibly through inhibiting the inflammatory response in the isoflurane-induced neuronal injury of rats. The protective effect of picroside II may be achieved by downregulating the expression of miR-195 and then inhibiting the inflammatory response.

Bilirubin-induced ER stress contributes to the inflammatory response and apoptosis in neuronal cells

2016 ◽  
Vol 91 (4) ◽  
pp. 1847-1858 ◽  
Author(s):  
Mohammed Qaisiya ◽  
Cristina Brischetto ◽  
Jana Jašprová ◽  
Libor Vitek ◽  
Claudio Tiribelli ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Er Stress ◽  
Neuronal Cells

scholarly journals Zika virus infection differentially affects genome-wide transcription in neuronal cells and myeloid dendritic cells

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0231049
Author(s):  
Tamina Park ◽  
Myung-gyun Kang ◽  
Seung-hwa Baek ◽  
Chang Hoon Lee ◽  
Daeui Park
Keyword(s):  
Dendritic Cells ◽  
Virus Infection ◽  
Zika Virus ◽  
Neuronal Cells ◽  
Myeloid Dendritic Cells ◽  
Zika Virus Infection ◽  
Genome Wide

scholarly journals A Novel Mechanism for Zika Virus Host-Cell Binding

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1101
Author(s):  
Courtney A. Rieder ◽  
Jonathan Rieder ◽  
Sebastién Sannajust ◽  
Diana Goode ◽  
Ramaz Geguchadze ◽  
...  
Keyword(s):  
Zika Virus ◽  
Neuronal Cells ◽  
Annexin V ◽  
Vero Cells ◽  
Western Hemisphere ◽  
Host Cells ◽  
E Protein ◽  
Clinical Presentations ◽  
Secondary Mechanism ◽  
Protein Cell

Zika virus (ZIKV) recently emerged in the Western Hemisphere with previously unrecognized or unreported clinical presentations. Here, we identify two putative binding mechanisms of ancestral and emergent ZIKV strains featuring the envelope (E) protein residue asparagine 154 (ASN154) and viral phosphatidylserine (PS). Synthetic peptides representing the region containing ASN154 from strains PRVABC59 (Puerto Rico 2015) and MR_766 (Uganda 1947) were exposed to neuronal cells and fibroblasts to model ZIKV E protein/cell interactions and bound MDCK or Vero cells and primary neurons significantly. Peptides significantly inhibited Vero cell infectivity by ZIKV strains MR_766 and PRVABC59, indicating that this region represents a putative binding mechanism of ancestral African ZIKV strains and emergent Western Hemisphere strains. Pretreatment of ZIKV strains MR_766 and PRVABC59 with the PS-binding protein annexin V significantly inhibited replication of PRVABC59 but not MR_766, suggesting that Western hemisphere strains may additionally be capable of utilizing PS-mediated entry to infect host cells. These data indicate that the region surrounding E protein ASN154 is capable of binding fibroblasts and primary neuronal cells and that PS-mediated entry may be a secondary mechanism for infectivity utilized by Western Hemisphere strains.

scholarly journals Pregnant Women Infected with Zika Virus Show Higher Viral Load and Immunoregulatory Cytokines Profile with CXCL10 Increase

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Elizabeth Camacho-Zavala ◽  
Clara Santacruz-Tinoco ◽  
Esteban Muñoz ◽  
Rommel Chacón-Salinas ◽  
Ma Isabel Salazar-Sanchez ◽  
...  
Keyword(s):  
Viral Load ◽  
Inflammatory Response ◽  
Pregnant Women ◽  
Inflammatory Cytokines ◽  
Zika Virus ◽  
Epidemiological Surveillance ◽  
Congenital Defects ◽  
Serum Samples ◽  
Zikv Infection ◽  
Anti Inflammatory

Background: Zika virus (ZIKV) infection during pregnancy usually shows only mild symptoms and is frequently subclinical. However, it can be vertically transmitted to the fetus, causing microcephaly and other congenital defects. During pregnancy, the immune environment modifications can alter the response to viruses in general and ZIKV in particular. Objective: To describe the role of pregnancy in the systemic pro- and anti-inflammatory response during symptomatic ZIKV infection. Materials and Methods: A multiplex assay was used to measure 25 cytokines, chemokines, and receptors in 110 serum samples from pregnant and nonpregnant women with and without ZIKV infection with and without symptoms. Samples were collected through an epidemiological surveillance system. Results: Samples from pregnant women with ZIKV infection showed a higher viral load but had similar profiles of inflammatory markers as compared with nonpregnant infected women, except for CXCL10 that was higher in infected pregnant women. Notably, the presence of ZIKV in pregnancy favored a regulatory profile by significantly increasing anti-inflammatory cytokines such as interleukin (IL)-10, receptors IL-1RA, and IL-2R, but only those pro-inflammatory cytokines such as IL-6, interferon (IFN)-α, IFN-γ and IL-17 that are essential for the antiviral response. Interestingly, there were no differences between symptomatic and weakly symptomatic ZIKV-infected groups. Conclusion: Our results revealed a systemic anti-inflammatory cytokine and chemokine profile that could participate in the control of the virus. The anti-inflammatory response in pregnant women infected with ZIKA was characterized by high CXCL10, a cytokine that has been correlated with congenital malformations.

scholarly journals Effects Amyloid Beta Peptide on the Inflammatory Response in Neuronal Cells

KSBB Journal ◽  
2013 ◽  
Vol 28 (4) ◽  
pp. 230-237
Author(s):  
Seon-A Jang ◽  
Hyun Jung Koo ◽  
Se Chan Kang ◽  
Eun-Hwa Sohn ◽  
Seung Namkoong
Keyword(s):  
Inflammatory Response ◽  
Amyloid Beta ◽  
Neuronal Cells ◽  
Amyloid Beta Peptide ◽  
Beta Peptide

scholarly journals 869: Zika virus can infect term human placentas devoid of the maternal inflammatory response

2018 ◽  
Vol 218 (1) ◽  
pp. S517-S518
Author(s):  
Diana Villazana-Kretzer ◽  
Stacey Schmiedecke ◽  
Peter G. Napolitano ◽  
Jennifer Stencel-Baerenwald ◽  
Michael Gale ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Zika Virus

scholarly journals Toll-like receptor 3 regulates Zika virus infection and associated host inflammatory response in primary human astrocytes

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0208543 ◽  
Author(s):  
Chet Raj Ojha ◽  
Myosotys Rodriguez ◽  
Mohan Kumar Muthu Karuppan ◽  
Jessica Lapierre ◽  
Fatah Kashanchi ◽  
...  
Keyword(s):  
Virus Infection ◽  
Inflammatory Response ◽  
Zika Virus ◽  
Toll Like Receptor ◽  
Human Astrocytes ◽  
Zika Virus Infection ◽  
Host Inflammatory Response
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