scholarly journals More DNA–Aptamers for Small Drugs: A Capture–SELEX Coupled with Surface Plasmon Resonance and High-Throughput Sequencing

2015 ◽  
Vol 17 (5) ◽  
pp. 326-333 ◽  
Author(s):  
Fabio M. Spiga ◽  
Paolo Maietta ◽  
Carlotta Guiducci
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
High Throughput Sequencing ◽  
Dna Aptamers

scholarly journals High-throughput imaging surface plasmon resonance biosensing based on ultrafast two-point spectral-dip tracking scheme

2020 ◽  
Vol 28 (14) ◽  
pp. 20624
Author(s):  
Youjun Zeng ◽  
Xueliang Wang ◽  
Jie Zhou ◽  
Ruibiao Miyan ◽  
Junle Qu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Imaging Surface Plasmon Resonance ◽  
High Throughput Imaging

scholarly journals Rapid and Label-Free Immunosensing of Shiga Toxin Subtypes with Surface Plasmon Resonance Imaging

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 280
Author(s):  
Bin Wang ◽  
Bosoon Park ◽  
Jing Chen ◽  
Xiaohua He
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Shiga Toxin ◽  
The United States ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Shiga Toxins

Shiga toxin-producing Escherichia coli (STEC) are responsible for gastrointestinal diseases reported in numerous outbreaks around the world as well as in the United States. Current detection methods have limitation to implement for rapid field-deployable detection with high volume of samples that are needed for regulatory purposes. Surface plasmon resonance imaging (SPRi) has proved to achieve rapid and label-free screening of multiple pathogens simultaneously, so it was evaluated in this work for the detection of Shiga toxins (Stx1a and Stx2a toxoids were used as the less toxic alternatives to Stx1 and Stx2, respectively). Multiple antibodies (Stx1pAb, Stx1-1mAb, Stx1-2mAb, Stx1d-3mAb, Stx1e-4mAb, Stx2pAb, Stx2-1mAb, Stx2-2mAb, and Stx2-10mAb) were spotted one by one by programed microarrayer, on the same high-throughput biochip with 50-nm gold film through multiple crosslinking and blocking steps to improve the orientation of antibodies on the biochip surface. Shiga toxins were detected based on the SPRi signal difference (ΔR) between immobilized testing antibodies and immunoglobulin G (IgG) control. Among the antibodies tested, Stx1pAb showed the highest sensitivity for Stx1 toxoid, with the limit of detection (LOD) of 50 ng/mL and detection time of 20 min. Both Stx2-1mAb and Stx2-2mAb exhibited high sensitivity for Stx2 toxoid. Furthermore, gold nanoparticles (GNPs) were used to amplify the SPRi signals of monoclonal antibodies in a sandwich platform. The LOD reached the level of picogram (pg)/mL with the help of GNP-antibody conjugate. This result proved that SPRi biochip with selected antibodies has the potential for rapid, high-throughput and multiplex detection of Shiga toxins.

High-throughput measurement of drug–cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 3213-3218 ◽  
Author(s):  
Vikramjeet Singh ◽  
Zhuo Li ◽  
Xiaotong Zhou ◽  
Xiaonan Xu ◽  
Jianghui Xu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Rate Constants ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Kinetic Rate ◽  
Kinetic Rate Constants

A high-throughput methodology for the measurement of drug–CD kinetic rate constants.

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