scholarly journals Creation of (R)-Amine Transaminase Activity within an α-Amino Acid Transaminase Scaffold

2020 ◽  
Vol 15 (2) ◽  
pp. 416-424 ◽  
Author(s):  
Moritz Voss ◽  
Chao Xiang ◽  
Jérémy Esque ◽  
Alberto Nobili ◽  
Marian J. Menke ◽  
...  
Keyword(s):  
Amino Acid ◽  
Transaminase Activity

Short-interval absorption and metabolism of some amino acids in Trypanosoma gambiense

Parasitology ◽  
1972 ◽  
Vol 64 (3) ◽  
pp. 379-387 ◽  
Author(s):  
L. H. Chappell ◽  
G. C. Southworth ◽  
C. P. Read
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Short Interval ◽  
Cysteic Acid ◽  
Transaminase Activity ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
High Level ◽  
Rate Limiting ◽  
Trypanosoma Gambiense

During 2 min incubations of Trypanosoma gambiense (bloodstream form) with [U-14C]glucose (1 mM) over 60% of absorbed label was detected in free alanine. In the presence of 12·5 mM unlabelled alanine, the amount of alanine synthesized from glucose was reduced by less than 10%. These data support previous observations on the high level of transaminase activity in African human trypanosomes.Alanine, aspartate and glutamate were metabolized to various other free amino acids whereas a significant amount of label derived from [14C]arginine could not be accounted for by amino acid assay.The sulphur containing amino acids, cysteic acid and taurine, were apparently synthesized from alanine, glutamate and arginine. The significance of these syntheses is poorly understood.Following incubations of trypanosomes for 2 min in exogenous amino acids the internal free pool became imbalanced due to accumulation and metabolism of the substrate amino acid.Evidence obtained indicated that the level of free endogenous glutamate may be rate limiting for the glutamate-pyruvate transaminase system.

Completing the folate biosynthesis pathway in Plasmodium falciparum: p-aminobenzoate is produced by a highly divergent promiscuous aminodeoxychorismate lyase

2013 ◽  
Vol 455 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Giovanni Magnani ◽  
Michela Lomazzi ◽  
Alessio Peracchi
Keyword(s):  
Plasmodium Falciparum ◽  
Amino Acid ◽  
Biosynthesis Pathway ◽  
Transaminase Activity ◽  
Antimalarial Therapy ◽  
Folate Biosynthesis ◽  
A Minor

We identified the aminodeoxychorismate lyase from Plasmodium falciparum. This enzyme participates in the biosynthesis of folate and could be a new target for antimalarial therapy. The enzyme has little similarity to its bacterial counterparts and shows a minor D-amino acid transaminase activity.

Amino-Acid Chromatography and Transaminase Activity in Denervation Atrophy

1960 ◽  
Vol 42 (6) ◽  
pp. 1008-1014 ◽  
Author(s):  
Pinckney J. Harman ◽  
J. L. Magnani ◽  
S. D. Sanwal ◽  
W. A. L. Thompson
Keyword(s):  
Amino Acid ◽  
Denervation Atrophy ◽  
Transaminase Activity

The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

1986 ◽  
Vol 44 ◽  
pp. 150-151
Author(s):  
M.K. Lamvik ◽  
L.L. Klatt
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Staining Pattern ◽  
Banding Patterns ◽  
Mass Thickness ◽  
New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

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