Hijacking the Heme Acquisition System of Pseudomonas aeruginosa for the Delivery of Phthalocyanine as an Antimicrobial

2019 ◽  
Vol 14 (7) ◽  
pp. 1637-1642 ◽  
Author(s):  
Yuma Shisaka ◽  
Yusuke Iwai ◽  
Shiho Yamada ◽  
Hiromu Uehara ◽  
Takehiko Tosha ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acquisition System ◽  
Heme Acquisition

Mechanism of Heme Uptake by Heme Acquisition System A

2011 ◽  
Vol 40 (4) ◽  
pp. 362-363 ◽  
Author(s):  
Shin-ichi Ozaki ◽  
Akira Nakahara ◽  
Takehiro Sato
Keyword(s):  
Acquisition System ◽  
Heme Uptake ◽  
System A ◽  
Heme Acquisition

scholarly journals Identification and Characterization of the Hemophore-Dependent Heme Acquisition System of Yersinia pestis

2001 ◽  
Vol 69 (11) ◽  
pp. 6707-6717 ◽  
Author(s):  
Maria-Silvia Rossi ◽  
Jacqueline D. Fetherston ◽  
Sylvie Létoffé ◽  
Elisabeth Carniel ◽  
Robert D. Perry ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Genomic Sequence ◽  
Protein Complexes ◽  
Receptor Gene ◽  
Heme Protein ◽  
Acquisition System ◽  
E Coli ◽  
Heme Acquisition ◽  
Genes Encoding ◽  
Iron Analysis

ABSTRACT Yersinia pestis possesses a heme-protein acquisition system (Hmu) that allows it to utilize heme and heme-protein complexes as the sole sources of iron. Analysis of the Y. pestisCO92 genomic sequence revealed a second heme-protein acquisition gene cluster that shares homology with the hemophore-dependent heme acquisition system (Has system) of Serratia marcescens. This locus consisted of thehasR yp receptor gene, thehasA yp hemophore gene, and genes encoding components of the HasAyp dedicated ABC transporter factor (hasDE yp ), as well as atonB homologue (hasB yp). By using a reconstituted secretion system in Escherichia coli, we showed that HasAyp is a secreted heme-binding protein and that expression of HasAyp is iron regulated in E. coli. The use of a transcriptional reporter fusion showed that the hasRADEB promoter is Fur regulated and has increased activity at 37°C. Hemoglobin utilization via the Hasyp system was studied with both E. coli and Y. pestis, for which hasand has hmu mutant strains were used. No contribution of the Has system to heme utilization was observed in either E. coli or Y. pestis under the conditions we tested. Previously it was shown that a deletion of the Hmu system had no effect on the virulence of Y. pestis in a mouse model of bubonic plague. An Hmu− Has− double mutant also retained full virulence in this model of infection. This report constitutes the first attempt to investigate the contribution of the hemophore-dependent heme acquisition system in bacterial pathogenicity.

scholarly journals Direct Hemin Transfer from IsdA to IsdC in the Iron-regulated Surface Determinant (Isd) Heme Acquisition System ofStaphylococcus aureus

2008 ◽  
Vol 283 (11) ◽  
pp. 6668-6676 ◽  
Author(s):  
Mengyao Liu ◽  
Wesley N. Tanaka ◽  
Hui Zhu ◽  
Gang Xie ◽  
David M. Dooley ◽  
...  
Keyword(s):  
Acquisition System ◽  
Ofstaphylococcus Aureus ◽  
Heme Acquisition
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