scholarly journals Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin inAspergillus nidulans

2018 ◽  
Vol 13 (11) ◽  
pp. 3193-3205 ◽  
Author(s):  
Michelle F. Grau ◽  
Ruth Entwistle ◽  
Yi-Ming Chiang ◽  
Manmeet Ahuja ◽  
C. Elizabeth Oakley ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Secondary Metabolite Gene Cluster

scholarly journals Apicidin F: Characterization and Genetic Manipulation of a New Secondary Metabolite Gene Cluster in the Rice Pathogen Fusarium fujikuroi

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e103336 ◽  
Author(s):  
Eva-Maria Niehaus ◽  
Slavica Janevska ◽  
Katharina W. von Bargen ◽  
Christian M. K. Sieber ◽  
Henning Harrer ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Secondary Metabolite ◽  
Genetic Manipulation ◽  
Fusarium Fujikuroi ◽  
Secondary Metabolite Gene Cluster

scholarly journals Evidence for horizontal transfer of a secondary metabolite gene cluster between fungi

2008 ◽  
Vol 9 (1) ◽  
pp. R18 ◽  
Author(s):  
Nora Khaldi ◽  
Jérôme Collemare ◽  
Marc-Henri Lebrun ◽  
Kenneth H Wolfe
Keyword(s):  
Gene Cluster ◽  
Secondary Metabolite ◽  
Horizontal Transfer ◽  
Secondary Metabolite Gene Cluster

scholarly journals Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death

2017 ◽  
Vol 114 (9) ◽  
pp. E1578-E1586 ◽  
Author(s):  
Yanhua Fan ◽  
Xi Liu ◽  
Nemat O. Keyhani ◽  
Guirong Tang ◽  
Yan Pei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Beauveria Bassiana ◽  
Negative Regulator ◽  
Bacterial Colony ◽  
Fusion Construct ◽  
Regulatory Cascade ◽  
Host Death

The regulatory network and biological functions of the fungal secondary metabolite oosporein have remained obscure. Beauveria bassiana has evolved the ability to parasitize insects and outcompete microbial challengers for assimilation of host nutrients. A novel zinc finger transcription factor, BbSmr1 (B. bassiana secondary metabolite regulator 1), was identified in a screen for oosporein overproduction. Deletion of Bbsmr1 resulted in up-regulation of the oosporein biosynthetic gene cluster (OpS genes) and constitutive oosporein production. Oosporein production was abolished in double mutants of Bbsmr1 and a second transcription factor, OpS3, within the oosporein gene cluster (ΔBbsmr1ΔOpS3), indicating that BbSmr1 acts as a negative regulator of OpS3 expression. Real-time quantitative PCR and a GFP promoter fusion construct of OpS1, the oosporein polyketide synthase, indicated that OpS1 is expressed mainly in insect cadavers at 24–48 h after death. Bacterial colony analysis in B. bassiana-infected insect hosts revealed increasing counts until host death, with a dramatic decrease (∼90%) after death that correlated with oosporein production. In vitro studies verified the inhibitory activity of oosporein against bacteria derived from insect cadavers. These results suggest that oosporein acts as an antimicrobial compound to limit microbial competition on B. bassiana-killed hosts, allowing the fungus to maximally use host nutrients to grow and sporulate on infected cadavers.

scholarly journals Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts

2018 ◽  
Vol 115 (43) ◽  
pp. 11030-11035 ◽  
Author(s):  
David J. Krause ◽  
Jacek Kominek ◽  
Dana A. Opulente ◽  
Xing-Xing Shen ◽  
Xiaofan Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Kluyveromyces Lactis ◽  
Phylogenetic Analyses ◽  
Iron Binding ◽  
Acid Biosynthesis ◽  
Transcription Factor Genes ◽  
Domains Of Life ◽  
Secondary Metabolite Gene Cluster

Secondary metabolites are key in how organisms from all domains of life interact with their environment and each other. The iron-binding molecule pulcherrimin was described a century ago, but the genes responsible for its production in budding yeasts have remained uncharacterized. Here, we used phylogenomic footprinting on 90 genomes across the budding yeast subphylum Saccharomycotina to identify the gene cluster associated with pulcherrimin production. Using targeted gene replacements in Kluyveromyces lactis, we characterized the four genes that make up the cluster, which likely encode two pulcherriminic acid biosynthesis enzymes, a pulcherrimin transporter, and a transcription factor involved in both biosynthesis and transport. The requirement of a functional putative transporter to utilize extracellular pulcherrimin-complexed iron demonstrates that pulcherriminic acid is a siderophore, a chelator that binds iron outside the cell for subsequent uptake. Surprisingly, we identified homologs of the putative transporter and transcription factor genes in multiple yeast genera that lacked the biosynthesis genes and could not make pulcherrimin, including the model yeast Saccharomyces cerevisiae. We deleted these previously uncharacterized genes and showed they are also required for pulcherrimin utilization in S. cerevisiae, raising the possibility that other genes of unknown function are linked to secondary metabolism. Phylogenetic analyses of this gene cluster suggest that pulcherrimin biosynthesis and utilization were ancestral to budding yeasts, but the biosynthesis genes and, subsequently, the utilization genes, were lost in many lineages, mirroring other microbial public goods systems that lead to the rise of cheater organisms.

scholarly journals Evolutionary Origins of the Fumonisin Secondary Metabolite Gene Cluster in Fusarium verticillioides and Aspergillus niger

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Nora Khaldi ◽  
Kenneth H. Wolfe
Keyword(s):  
Comparative Genomics ◽  
Aspergillus Niger ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Horizontal Transfer ◽  
Fusarium Verticillioides ◽  
Gene Clusters ◽  
Evolutionary Origins ◽  
Species Specific ◽  
Secondary Metabolite Gene Cluster

The secondary metabolite gene clusters of euascomycete fungi are among the largest known clusters of functionally related genes in eukaryotes. Most of these clusters are species specific or genus specific, and little is known about how they are formed during evolution. We used a comparative genomics approach to study the evolutionary origins of a secondary metabolite cluster that synthesizes a polyketide derivative, namely, the fumonisin (FUM) cluster of Fusarium verticillioides, and that of Aspergillus niger another fumonisin (fumonisin B) producing species. We identified homologs in other euascomycetes of the Fusarium verticillioides FUM genes and their flanking genes. We discuss four models for the origin of the FUM cluster in Fusarium verticillioides and argue that two of these are plausible: (i) assembly by relocation of initially scattered genes in a recent Fusarium verticillioides; or (ii) horizontal transfer of the FUM cluster from a distantly related Sordariomycete species. We also propose that the FUM cluster was horizontally transferred into Aspergillus niger, most probably from a Sordariomycete species.

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

scholarly journals Bioactive Molecules from Mangrove Streptomyces qinglanensis 172205

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 255
Author(s):  
Dongbo Xu ◽  
Erli Tian ◽  
Fandong Kong ◽  
Kui Hong
Keyword(s):  
Gene Cluster ◽  
Secondary Metabolite ◽  
Biosynthetic Gene Cluster ◽  
Bioactive Molecules ◽  
Spectroscopic Methods ◽  
Biosynthetic Gene ◽  
Electronic Circular Dichroism ◽  
Chemical Structures ◽  
New Compounds ◽  
Antiproliferative Activities

Five new compounds 15R-17,18-dehydroxantholipin (1), (3E,5E,7E)-3-methyldeca-3,5,7-triene-2,9-dione (2) and qinlactone A–C (3–5) were identified from mangrove Streptomyces qinglanensis 172205 with “genetic dereplication,” which deleted the highly expressed secondary metabolite-enterocin biosynthetic gene cluster. The chemical structures were established by spectroscopic methods, and the absolute configurations were determined by electronic circular dichroism (ECD). Compound 1 exhibited strong anti-microbial and antiproliferative bioactivities, while compounds 2–4 showed weak antiproliferative activities.

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