The Unique Cofactor Region of Zika Virus NS2B–NS3 Protease Facilitates Cleavage of Key Host Proteins

2018 ◽  
Vol 13 (9) ◽  
pp. 2398-2405 ◽  
Author(s):  
Maureen E. Hill ◽  
Anil Kumar ◽  
James A. Wells ◽  
Tom C. Hobman ◽  
Olivier Julien ◽  
...  
Keyword(s):  
Zika Virus ◽  
Host Proteins ◽  
Ns3 Protease

scholarly journals Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3732
Author(s):  
Agnieszka Dabrowska ◽  
Aleksandra Milewska ◽  
Joanna Ner-Kluza ◽  
Piotr Suder ◽  
Krzysztof Pyrc
Keyword(s):  
Mass Spectrometry ◽  
Zika Virus ◽  
Viral Infections ◽  
Protein Detection ◽  
Extensive Discussion ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Infected Cells ◽  
Ns3 Protease ◽  
To Receive

Mass spectrometry (MS) used in proteomic approaches is able to detect hundreds of proteins in a single assay. Although undeniable high analytical power of MS, data acquired sometimes lead to confusing results, especially during a search of very selective, unique interactions in complex biological matrices. Here, we would like to show an example of such confusing data, providing an extensive discussion on the observed phenomenon. Our investigations focus on the interaction between the Zika virus NS3 protease, which is essential for virus replication. This enzyme is known for helping to remodel the microenvironment of the infected cells. Several reports show that this protease can process cellular substrates and thereby modify cellular pathways that are important for the virus. Herein, we explored some of the targets of NS3, clearly shown by proteomic techniques, as processed during infection. Unfortunately, we could not confirm the biological relevance of protein targets for viral infections detected by MS. Thus, although mass spectrometry is highly sensitive and useful in many instances, also being able to show directions where cell/virus interaction occurs, we believe that deep recognition of their biological role is essential to receive complete insight into the investigated process.

Flavonoids from Pterogyne nitens as Zika virus NS2B-NS3 protease inhibitors

2021 ◽  
Vol 109 ◽  
pp. 104719
Author(s):  
Caroline Sprengel Lima ◽  
Melina Mottin ◽  
Leticia Ribeiro de Assis ◽  
Nathalya Cristina de Moraes Roso Mesquita ◽  
Bruna Katiele de Paula Sousa ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Zika Virus ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

NS2B‐NS3 protease inhibitors as promising compounds in the development of antivirals against Zika virus: A systematic review

2021 ◽  
Author(s):  
Damiana Antônia de Fátima Nunes ◽  
Felipe Rocha da Silva Santos ◽  
Sara Thamires Dias da Fonseca ◽  
William Gustavo de Lima ◽  
Waleska Stephanie da Cruz Nizer ◽  
...  
Keyword(s):  
Systematic Review ◽  
Protease Inhibitors ◽  
Zika Virus ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

scholarly journals Structural Insights into the Inhibition of Zika Virus NS2B-NS3 Protease by a Small-Molecule Inhibitor

Structure ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 555-564.e3 ◽  
Author(s):  
Yan Li ◽  
Zhenzhen Zhang ◽  
Wint Wint Phoo ◽  
Ying Ru Loh ◽  
Rong Li ◽  
...  
Keyword(s):  
Small Molecule ◽  
Zika Virus ◽  
Small Molecule Inhibitor ◽  
Molecule Inhibitor ◽  
Ns3 Protease ◽  
Structural Insights

scholarly journals Identification and structural characterization of small molecule fragments targeting Zika virus NS2B-NS3 protease

2020 ◽  
Vol 175 ◽  
pp. 104707 ◽  
Author(s):  
Jun Ping Quek ◽  
Shuang Liu ◽  
Zhenzhen Zhang ◽  
Yan Li ◽  
Elizabeth Yihui Ng ◽  
...  
Keyword(s):  
Small Molecule ◽  
Structural Characterization ◽  
Zika Virus ◽  
Ns3 Protease

MD simulations reveal alternate conformations of the oxyanion hole in the Zika virus NS2B/NS3 protease

2019 ◽  
Vol 88 (2) ◽  
pp. 345-354
Author(s):  
Jinhong Ren ◽  
Hyun Lee ◽  
Alpa Kotak ◽  
Michael E. Johnson
Keyword(s):  
Zika Virus ◽  
Md Simulations ◽  
Oxyanion Hole ◽  
Ns3 Protease

Novel antiviral activity and mechanism of bromocriptine as a Zika virus NS2B-NS3 protease inhibitor

2017 ◽  
Vol 141 ◽  
pp. 29-37 ◽  
Author(s):  
Jasper Fuk-Woo Chan ◽  
Kenn Ka-Heng Chik ◽  
Shuofeng Yuan ◽  
Cyril Chik-Yan Yip ◽  
Zheng Zhu ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Protease Inhibitor ◽  
Zika Virus ◽  
Ns3 Protease

scholarly journals Structural characterization of the linked NS2B-NS3 protease of Zika virus

FEBS Letters ◽  
2017 ◽  
Vol 591 (15) ◽  
pp. 2338-2347 ◽  
Author(s):  
Yan Li ◽  
Wint Wint Phoo ◽  
Ying Ru Loh ◽  
Zhenzhen Zhang ◽  
Elizabeth Yihui Ng ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Zika Virus ◽  
Ns3 Protease
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