Biosynthesis of (−)-5-Hydroxy-equol and 5-Hydroxy-dehydroequol from Soy Isoflavone, Genistein Using Microbial Whole Cell Bioconversion

2017 ◽  
Vol 12 (11) ◽  
pp. 2883-2890 ◽  
Author(s):  
Pyung-Gang Lee ◽  
Joonwon Kim ◽  
Eun-Jung Kim ◽  
Sang-Hyuk Lee ◽  
Kwon-Young Choi ◽  
...  
Keyword(s):  
Whole Cell ◽  
Soy Isoflavone ◽  
Whole Cell Bioconversion

scholarly journals High-Level Conversion of l-lysine into Cadaverine by Escherichia coli Whole Cell Biocatalyst Expressing Hafnia alvei l-lysine Decarboxylase

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1184 ◽  
Author(s):  
Kim ◽  
Baritugo ◽  
Oh ◽  
Kang ◽  
Jung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Pyridoxal Phosphate ◽  
Lysine Decarboxylase ◽  
Hafnia Alvei ◽  
Whole Cell ◽  
E Coli ◽  
Whole Cell Biocatalyst ◽  
High Level ◽  
Whole Cell Bioconversion

Cadaverine is a C5 diamine monomer used for the production of bio-based polyamide 510. Cadaverine is produced by the decarboxylation of l-lysine using a lysine decarboxylase (LDC). In this study, we developed recombinant Escherichia coli strains for the expression of LDC from Hafnia alvei. The resulting recombinant XBHaLDC strain was used as a whole cell biocatalyst for the high-level bioconversion of l-lysine into cadaverine without the supplementation of isopropyl β-d-1-thiogalactopyranoside (IPTG) for the induction of protein expression and pyridoxal phosphate (PLP), a key cofactor for an LDC reaction. The comparison of results from enzyme characterization of E. coli and H. alvei LDC revealed that H. alvei LDC exhibited greater bioconversion ability than E. coli LDC due to higher levels of protein expression in all cellular fractions and a higher specific activity at 37 °C (1825 U/mg protein > 1003 U/mg protein). The recombinant XBHaLDC and XBEcLDC strains were constructed for the high-level production of cadaverine. Recombinant XBHaLDC produced a 1.3-fold higher titer of cadaverine (6.1 g/L) than the XBEcLDC strain (4.8 g/L) from 10 g/L of l-lysine. Furthermore, XBHaLDC, concentrated to an optical density (OD600) of 50, efficiently produced 136 g/L of cadaverine from 200 g/L of l-lysine (97% molar yield) via an IPTG- and PLP-free whole cell bioconversion reaction. Cadaverine synthesized via a whole cell biocatalyst reaction using XBHaLDC was purified to polymer grade, and purified cadaverine was successfully used for the synthesis of polyamide 510. In conclusion, an IPTG- and PLP-free whole cell bioconversion process of l-lysine into cadaverine, using recombinant XBHaLDC, was successfully utilized for the production of bio-based polyamide 510, which has physical and thermal properties similar to polyamide 510 synthesized from chemical-grade cadaverine.

scholarly journals Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Junyoung Kim ◽  
Hyung-Min Seo ◽  
Shashi Kant Bhatia ◽  
Hun-Seok Song ◽  
Jung-Ho Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Bioconversion

Enhanced bioconversion rate and released substrate inhibition in (R)-phenylephrine whole-cell bioconversion via partial acetone treatment

2016 ◽  
Vol 86 ◽  
pp. 34-38 ◽  
Author(s):  
Shu-Chen Kan ◽  
Chi-Zong Zang ◽  
Chiung-Wen Yeh ◽  
Wei-Feng Chang ◽  
Chia-Chi Lin ◽  
...  
Keyword(s):  
Substrate Inhibition ◽  
Whole Cell ◽  
Acetone Treatment ◽  
Whole Cell Bioconversion

A Multiphasic Hollow Fiber Reactor for the Whole-Cell Bioconversion of 2-Methyl-1,3-propanediol to (R)-β-Hydroxyisobutyric Acid

2001 ◽  
Vol 17 (3) ◽  
pp. 468-473 ◽  
Author(s):  
R. Leon ◽  
D.M.F. Prazeres ◽  
P. Fernandes ◽  
F. Molinari ◽  
J.M.S. Cabral
Keyword(s):  
Hollow Fiber ◽  
Whole Cell ◽  
Hollow Fiber Reactor ◽  
Hydroxyisobutyric Acid ◽  
Whole Cell Bioconversion

Nonionic Micelles Promote Whole Cell Bioconversion of Aromatic Substrates in an Aqueous Environment

Langmuir ◽  
2002 ◽  
Vol 18 (16) ◽  
pp. 6015-6020 ◽  
Author(s):  
Debora Berti ◽  
Demetrio Randazzo ◽  
Fabrizio Briganti ◽  
Andrea Scozzafava ◽  
Patrizia Di Gennaro ◽  
...  
Keyword(s):  
Aqueous Environment ◽  
Whole Cell ◽  
Aromatic Substrates ◽  
Whole Cell Bioconversion

Whole-cell bioconversion of naringenin to high added value hydroxylated compounds using Yarrowia lipolytica 2.2ab in surface and liquid cultures

2020 ◽  
Vol 43 (7) ◽  
pp. 1219-1230
Author(s):  
Christian Hernández-Guzmán ◽  
Arely Prado-Barragán ◽  
Miquel Gimeno ◽  
Angélica Román-Guerrero ◽  
Olga Miriam Rutiaga-Quiñones ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Added Value ◽  
Whole Cell ◽  
Liquid Cultures ◽  
Whole Cell Bioconversion

Whole-cell bioconversion of β-sitosterol in aqueous–organic two-phase systems

2001 ◽  
Vol 11 (4-6) ◽  
pp. 579-585 ◽  
Author(s):  
A. Cruz ◽  
P. Fernandes ◽  
J.M.S. Cabral ◽  
H.M. Pinheiro
Keyword(s):  
Two Phase ◽  
Whole Cell ◽  
Phase Systems ◽  
Whole Cell Bioconversion
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