Accurate Detection of Adenylation Domain Functions in Nonribosomal Peptide Synthetases by an Enzyme-linked Immunosorbent Assay System Using Active Site-directed Probes for Adenylation Domains

2015 ◽  
Vol 10 (12) ◽  
pp. 2816-2826 ◽  
Author(s):  
Fumihiro Ishikawa ◽  
Kengo Miyamoto ◽  
Sho Konno ◽  
Shota Kasai ◽  
Hideaki Kakeya
Keyword(s):  
Immunosorbent Assay ◽  
Active Site ◽  
Assay System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adenylation Domain ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Accurate Detection

scholarly journals Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases

2015 ◽  
Vol 51 (12) ◽  
pp. 2262-2265 ◽  
Author(s):  
Sho Konno ◽  
Fumihiro Ishikawa ◽  
Takehiro Suzuki ◽  
Naoshi Dohmae ◽  
Michael D. Burkart ◽  
...  
Keyword(s):  
Natural Product ◽  
Active Site ◽  
Nonribosomal Peptide Synthetase ◽  
Protein Labeling ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Peptide Synthetase ◽  
A Domains

Active site-directed proteomic probes coupled to the 5′-O-N-(aminoacyl)sulfamoyladenosine (AMS) scaffold with a clickable benzophenone functionality selectively target nonribosomal peptide synthetase (NRPS) adenylation (A) domains in natural product producer proteomes by ligand-directed protein labeling.

A Multiple-Labeling Strategy for Nonribosomal Peptide Synthetases Using Active-Site-Directed Proteomic Probes for Adenylation Domains

ChemBioChem ◽  
2015 ◽  
Vol 16 (18) ◽  
pp. 2590-2594 ◽  
Author(s):  
Fumihiro Ishikawa ◽  
Takehiro Suzuki ◽  
Naoshi Dohmae ◽  
Hideaki Kakeya
Keyword(s):  
Active Site ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases

scholarly journals Exploring the Adenylation Domain Repertoire of Nonribosomal Peptide Synthetases Using an Ensemble of Sequence-Search Methods

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e65926 ◽  
Author(s):  
Guillermin Agüero-Chapin ◽  
Reinaldo Molina-Ruiz ◽  
Emanuel Maldonado ◽  
Gustavo de la Riva ◽  
Aminael Sánchez-Rodríguez ◽  
...  
Keyword(s):  
Adenylation Domain ◽  
Search Methods ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Sequence Search

Microfluidic tectonics platform: A colorimetric, disposable botulinum toxin enzyme-linked immunosorbent assay system

2004 ◽  
Vol 25 (1011) ◽  
pp. 1705-1713 ◽  
Author(s):  
Jaisree Moorthy ◽  
Glennys A. Mensing ◽  
Dongshin Kim ◽  
Swomitra Mohanty ◽  
David T. Eddington ◽  
...  
Keyword(s):  
Botulinum Toxin ◽  
Immunosorbent Assay ◽  
Assay System ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Nonprocessive [2 + 2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases

2012 ◽  
Vol 109 (15) ◽  
pp. 5681-5686 ◽  
Author(s):  
A. Chhabra ◽  
A. S. Haque ◽  
R. K. Pal ◽  
A. Goyal ◽  
R. Rai ◽  
...  
Keyword(s):  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases
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