scholarly journals Wall teichoic acids prevent antibody binding to epitopes within the cell wall of Staphylococcus aureus

2015 ◽  
Vol 11 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Samir Gautam ◽  
Taehan Kim ◽  
Evan Lester ◽  
Deeksha Deep ◽  
David A. Spiegel
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Antibody Binding ◽  
Teichoic Acids ◽  
Wall Teichoic Acids

scholarly journals Dual Roles of FmtA in Staphylococcus aureus Cell Wall Biosynthesis and Autolysis

2012 ◽  
Vol 56 (7) ◽  
pp. 3797-3805 ◽  
Author(s):  
Aneela Qamar ◽  
Dasantila Golemi-Kotra
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Wall Stress ◽  
Microscopy Study ◽  
Dual Roles ◽  
Content Type ◽  
Teichoic Acids ◽  
Low Concentrations ◽  
High Concentrations ◽  
Wall Teichoic Acids

ABSTRACTThefmtAgene is a member of theStaphylococcus aureuscore cell wall stimulon. The FmtA protein interacts with β-lactams through formation of covalent species. Here, we show that FmtA has weakd-Ala-d-Ala-carboxypeptidase activity and is capable of covalently incorporating C14-Gly into cell walls. The fluorescence microscopy study showed that the protein is localized to the cell division septum. Furthermore, we show that wall teichoic acids interact specifically with FmtA and mediate recruitment of FmtA to theS. aureuscell wall. Subjection ofS. aureusto FmtA concentrations of 0.1 μM or less induces autolysis and biofilm production. This effect requires the presence of wall teichoic acids. At FmtA concentrations greater than 0.2 μM, autolysis and biofilm formation inS. aureusare repressed and growth is enhanced. Our findings indicate dual roles of FmtA inS. aureusgrowth, whereby at low concentrations, FmtA may modulate the activity of the major autolysin (AtlA) ofS. aureusand, at high concentrations, may participate in synthesis of cell wall peptidoglycan. These two roles of FmtA may reflect dual functions of FmtA in the absence and presence of cell wall stress, respectively.

scholarly journals Staphylococcus aureus Mutants Lacking the LytR-CpsA-Psr Family of Enzymes Release Cell Wall Teichoic Acids into the Extracellular Medium

2013 ◽  
Vol 195 (20) ◽  
pp. 4650-4659 ◽  
Author(s):  
Y. G. Y. Chan ◽  
M. B. Frankel ◽  
V. Dengler ◽  
O. Schneewind ◽  
D. Missiakas
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Extracellular Medium ◽  
Teichoic Acids ◽  
Wall Teichoic Acids

Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus

2016 ◽  
pp. 201-213 ◽  
Author(s):  
Gonçalo Covas ◽  
Filipa Vaz ◽  
Gabriela Henriques ◽  
Mariana G. Pinho ◽  
Sérgio R. Filipe
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Teichoic Acids ◽  
Wall Teichoic Acids

Cell wall teichoic acids of Bacillus licheniformis VKM B-511T, Bacillus pumilus VKM B-508T, and other strains previously assigned to Bacillus pumilus

Microbiology ◽  
2012 ◽  
Vol 81 (4) ◽  
pp. 425-434 ◽  
Author(s):  
G. M. Streshinskaya ◽  
A. S. Shashkov ◽  
Yu. I. Kozlova ◽  
E. M. Tul’skaya ◽  
E. B. Kudryashova ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Licheniformis ◽  
Bacillus Pumilus ◽  
Teichoic Acids ◽  
Wall Teichoic Acids

New structures and composition of cell wall teichoic acids from Nocardiopsis synnemataformans, Nocardiopsis halotolerans, Nocardiopsis composta and Nocardiopsis metallicus: a chemotaxonomic value

2014 ◽  
Vol 106 (6) ◽  
pp. 1105-1117 ◽  
Author(s):  
Elena M. Tul’skaya ◽  
Alexander S. Shashkov ◽  
Galina M. Streshinskaya ◽  
Natalia V. Potekhina ◽  
Ludmila I. Evtushenko
Keyword(s):  
Cell Wall ◽  
Teichoic Acids ◽  
Wall Teichoic Acids

scholarly journals Biosynthesis of the Unique Wall Teichoic Acid of Staphylococcus aureus Lineage ST395

mBio ◽  
2014 ◽  
Vol 5 (2) ◽  
Author(s):  
Volker Winstel ◽  
Patricia Sanchez-Carballo ◽  
Otto Holst ◽  
Guoqing Xia ◽  
Andreas Peschel
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Biosynthesis Pathway ◽  
Glycerol Phosphate ◽  
Coagulase Negative Staphylococci ◽  
Content Type ◽  
Gram Positive Bacteria ◽  
Wall Teichoic Acids

ABSTRACT The major clonal lineages of the human pathogen Staphylococcus aureus produce cell wall-anchored anionic poly-ribitol-phosphate (RboP) wall teichoic acids (WTA) substituted with d-Alanine and N-acetyl-d-glucosamine. The phylogenetically isolated S. aureus ST395 lineage has recently been found to produce a unique poly-glycerol-phosphate (GroP) WTA glycosylated with N-acetyl-d-galactosamine (GalNAc). ST395 clones bear putative WTA biosynthesis genes on a novel genetic element probably acquired from coagulase-negative staphylococci (CoNS). We elucidated the ST395 WTA biosynthesis pathway and identified three novel WTA biosynthetic genes, including those encoding an α-O-GalNAc transferase TagN, a nucleotide sugar epimerase TagV probably required for generation of the activated sugar donor substrate for TagN, and an unusually short GroP WTA polymerase TagF. By using a panel of mutants derived from ST395, the GalNAc residues carried by GroP WTA were found to be required for infection by the ST395-specific bacteriophage Φ187 and to play a crucial role in horizontal gene transfer of S. aureus pathogenicity islands (SaPIs). Notably, ectopic expression of ST395 WTA biosynthesis genes rendered normal S. aureus susceptible to Φ187 and enabled Φ187-mediated SaPI transfer from ST395 to regular S. aureus. We provide evidence that exchange of WTA genes and their combination in variable, mosaic-like gene clusters have shaped the evolution of staphylococci and their capacities to undergo horizontal gene transfer events. IMPORTANCE The structural highly diverse wall teichoic acids (WTA) are cell wall-anchored glycopolymers produced by most Gram-positive bacteria. While most of the dominant Staphylococcus aureus lineages produce poly-ribitol-phosphate WTA, the recently described ST395 lineage produces a distinct poly-glycerol-phosphate WTA type resembling the WTA backbone of coagulase-negative staphylococci (CoNS). Here, we analyzed the ST395 WTA biosynthesis pathway and found new types of WTA biosynthesis genes along with an evolutionary link between ST395 and CoNS, from which the ST395 WTA genes probably originate. The elucidation of ST395 WTA biosynthesis will help to understand how Gram-positive bacteria produce highly variable WTA types and elucidate functional consequences of WTA variation.

scholarly journals Colony Spreading in Staphylococcus aureus

2006 ◽  
Vol 189 (6) ◽  
pp. 2553-2557 ◽  
Author(s):  
Chikara Kaito ◽  
Kazuhisa Sekimizu
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Agar ◽  
Clinical Isolates ◽  
Wild Type ◽  
Teichoic Acids ◽  
Colony Spreading ◽  
Different Strains ◽  
Wall Teichoic Acids

ABSTRACT Wild-type Staphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of the dltABCD operon, which functions at the step of d-alanine addition to teichoic acids, and of the tagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids and d-alanylation of teichoic acids are required for colony spreading.

The structures of the cell wall teichoic acids from the thermophilic microorganism Geobacillus thermoleovorans strain Fango

2006 ◽  
Vol 341 (15) ◽  
pp. 2613-2618 ◽  
Author(s):  
Serena Leone ◽  
Antonio Molinaro ◽  
Ida Romano ◽  
Barbara Nicolaus ◽  
Rosa Lanzetta ◽  
...  
Keyword(s):  
Cell Wall ◽  
Thermophilic Microorganism ◽  
Teichoic Acids ◽  
Geobacillus Thermoleovorans ◽  
Wall Teichoic Acids
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