Blue Light Activated Rapamycin for Optical Control of Protein Dimerization in Cells and Zebrafish Embryos

2021 ◽  
Author(s):  
Taylor M. Courtney ◽  
Kristie E. Darrah ◽  
Trevor J. Horst ◽  
Michael Tsang ◽  
Alexander Deiters
Keyword(s):  
Blue Light ◽  
Optical Control ◽  
Zebrafish Embryos ◽  
Protein Dimerization ◽  
In Cells

scholarly journals Safe-by-Design CuO Nanoparticles via Fe-Doping, Cu–O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos

ACS Nano ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 501-515 ◽  
Author(s):  
Hendrik Naatz ◽  
Sijie Lin ◽  
Ruibin Li ◽  
Wen Jiang ◽  
Zhaoxia Ji ◽  
...  
Keyword(s):  
Bond Length ◽  
Cuo Nanoparticles ◽  
Biological Assessment ◽  
Zebrafish Embryos ◽  
Length Variation ◽  
Fe Doping ◽  
Safe By Design ◽  
In Cells

scholarly journals Optical Control of Metal Ion Probes in Cells and Zebrafish Using Highly Selective DNAzymes Conjugated to Upconversion Nanoparticles

2018 ◽  
Vol 140 (50) ◽  
pp. 17656-17665 ◽  
Author(s):  
Zhenglin Yang ◽  
Kang Yong Loh ◽  
Yueh-Te Chu ◽  
Ruopei Feng ◽  
Nitya Sai Reddy Satyavolu ◽  
...  
Keyword(s):  
Metal Ion ◽  
Optical Control ◽  
Upconversion Nanoparticles ◽  
In Cells

The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo

Development ◽  
1992 ◽  
Vol 116 (4) ◽  
pp. 1021-1032 ◽  
Author(s):  
S. Schulte-Merker ◽  
R.K. Ho ◽  
B.G. Herrmann ◽  
C. Nusslein-Volhard
Keyword(s):  
Early Development ◽  
Nuclear Localization ◽  
Protein Product ◽  
Early Embryo ◽  
Paraxial Mesoderm ◽  
Zebrafish Embryos ◽  
Brachydanio Rerio ◽  
Cloning And Sequencing ◽  
Yolk Syncytial Layer ◽  
In Cells

Embryos mutant for the T gene, in mice, make insufficient mesoderm and fail to develop a notochord. We report the cloning and sequencing of the T gene in the zebrafish (Brachydanio rerio) and show the nuclear localization of the protein product. Both RNA and protein are found in cells of the germ ring, including enveloping layer cells, prior to and during gastrulation of zebrafish embryos. Nuclei of the yolk syncytial layer do not express Zf-T. High levels of expression are maintained throughout early development in the notochord, while in paraxial mesoderm cells the gene is turned off during gastrulation. Exposure of animal cap cells to activinA induces Zf-T expression, as does transplantation into the germ ring.

scholarly journals Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA

2019 ◽  
Author(s):  
Wenyuan Zhou ◽  
Wes Brown ◽  
Anirban Bardhan ◽  
Michael Delaney ◽  
Amber S. Ilk ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Zebrafish Embryos ◽  
Complete Suppression ◽  
Guide Rna ◽  
Guide Rnas ◽  
Ribonucleoprotein Complexes ◽  
Conditional Control ◽  
Rapid Restoration ◽  
Spatiotemporal Control ◽  
In Cells

AbstractWe developed a new method for conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos via photochemically activated, caged guide RNAs. Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout the 5’-protospacer region with caged nucleobases during synthesis. Caging confers complete suppression of gRNA:target dsDNA hybridization and rapid restoration of CRISPR/Cas9 function upon optical activation. This tool offers simplicity and complete programmability in design, high spatiotemporal specificity in cells and zebrafish embryos, excellent off to on switching, and stability by preserving the ability to form Cas9:gRNA ribonucleoprotein complexes. caged gRNAs are novel tools for conditional control of gene editing thereby enabling the investigation of spatiotemporally complex physiological events by obtaining a better understanding of dynamic gene regulation.

Sodium Cholate-Templated Blue Light-Emitting Ag Subnanoclusters: In Vivo Toxicity and Imaging in Zebrafish Embryos

2015 ◽  
Vol 7 (3) ◽  
pp. 1422-1430 ◽  
Author(s):  
Shanmugam Chandirasekar ◽  
Chandramouli Chandrasekaran ◽  
Thangavel Muthukumarasamyvel ◽  
Ganapasam Sudhandiran ◽  
Nagappan Rajendiran
Keyword(s):  
Blue Light ◽  
Sodium Cholate ◽  
Zebrafish Embryos ◽  
Light Emitting ◽  
In Vivo Toxicity

scholarly journals Optical Control of Protein–Protein Interactions via Blue Light-Induced Domain Swapping

Biochemistry ◽  
2014 ◽  
Vol 53 (30) ◽  
pp. 5008-5016 ◽  
Author(s):  
Jakeb M. Reis ◽  
Darcy C. Burns ◽  
G. Andrew Woolley
Keyword(s):  
Blue Light ◽  
Protein Interactions ◽  
Domain Swapping ◽  
Optical Control ◽  
Protein Protein Interactions

Single-Embryo Metabolomics and Systematic Prediction of Developmental Stage in Zebrafish

2011 ◽  
Vol 66 (3-4) ◽  
pp. 191-198 ◽  
Author(s):  
Shunsuke Hayashi ◽  
Mako Yoshida ◽  
Toshinobu Fujiwara ◽  
Shingo Maegawa ◽  
Eiichiro Fukusaki
Keyword(s):  
Gene Expression ◽  
Developmental Stage ◽  
Metabolic Profiling ◽  
Zebrafish Embryos ◽  
Rate Of Development ◽  
End Products ◽  
Different Temperatures ◽  
Living Organisms ◽  
In Cells

Metabolites, the end products of gene expression in living organisms, are tightly correlated with an organism’s development and growth. Thus, metabolic profiling is a potentially important tool for understanding the events that have occurred in cells, tissues, and individual organisms. Here, we present a method for predicting the developmental stage of zebrafish embryos using novel metabolomic non-target fingerprints of “single-embryos”. With this method, we observed the rate of development at different temperatures. Our results suggest that this method allows us to analyse the condition, or distinguish the genotype, of singleembryos before expression of their ultimate phenotype

scholarly journals Bioorthogonal mRNA labeling at the poly(A) tail for imaging localization and dynamics in live zebrafish embryos

2020 ◽  
Vol 11 (11) ◽  
pp. 3089-3095 ◽  
Author(s):  
Kim J. Westerich ◽  
Karthik S. Chandrasekaran ◽  
Theresa Gross-Thebing ◽  
Nadine Kueck ◽  
Erez Raz ◽  
...  
Keyword(s):  
Live Imaging ◽  
Zebrafish Embryos ◽  
In Cells

Live imaging of mRNA in cells and organisms is important for understanding the dynamic aspects underlying its function.

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