A New Bacmid for Customized Protein Glycosylation Pathway Engineering in the Baculovirus-Insect Cell System

2021 ◽  
Author(s):  
Ajay B. Maghodia ◽  
Christoph Geisler ◽  
Donald L. Jarvis
Keyword(s):  
Insect Cell ◽  
Cell System ◽  
Protein Glycosylation ◽  
Pathway Engineering ◽  
Glycosylation Pathway

scholarly journals CRISPR-Cas9 vectors for genome editing and host engineering in the baculovirus–insect cell system

2017 ◽  
Vol 114 (34) ◽  
pp. 9068-9073 ◽  
Author(s):  
Hideaki Mabashi-Asazuma ◽  
Donald L. Jarvis
Keyword(s):  
Genome Editing ◽  
Insect Cell ◽  
Insect Cells ◽  
Basic Research ◽  
Cell System ◽  
Protein Glycosylation ◽  
Cell Protein ◽  
Site Specific ◽  
Insect Cell Lines ◽  
Cell Gene

The baculovirus–insect cell system (BICS) has been widely used to produce many different recombinant proteins for basic research and is being used to produce several biologics approved for use in human or veterinary medicine. Early BICS were technically complex and constrained by the relatively primordial nature of insect cell protein glycosylation pathways. Since then, recombination has been used to modify baculovirus vectors—which has simplified the system—and transform insect cells, which has enhanced its protein glycosylation capabilities. Now, CRISPR-Cas9 tools for site-specific genome editing are needed to facilitate further improvements in the BICS. Thus, in this study, we used various insect U6 promoters to construct CRISPR-Cas9 vectors and assessed their utility for site-specific genome editing in two insect cell lines commonly used as hosts in the BICS. We demonstrate the use of CRISPR-Cas9 to edit an endogenous insect cell gene and alter protein glycosylation in the BICS.

Expression of human plasminogen cDNA in a baculovirus vector-infected insect cell system

1989 ◽  
Vol 271 (2) ◽  
pp. 390-399 ◽  
Author(s):  
Joann Whitefleet-Smith ◽  
Elliot Rosen ◽  
James McLinden ◽  
Victoria A. Ploplis ◽  
Malcolm J. Fraser ◽  
...  
Keyword(s):  
Insect Cell ◽  
Cell System ◽  
Baculovirus Vector ◽  
Infected Insect ◽  
Human Plasminogen

scholarly journals Expression of a Drosophila GABA receptor in a baculovirus insect cell system

FEBS Letters ◽  
1993 ◽  
Vol 335 (3) ◽  
pp. 315-318 ◽  
Author(s):  
Hwa-Jung Lee ◽  
Thomas Rocheleau ◽  
Hai-Guang Zhang ◽  
Meyer B. Jackson ◽  
Richard H. ffrench-Constant
Keyword(s):  
Insect Cell ◽  
Gaba Receptor ◽  
Cell System

scholarly journals Mgat5 Deficiency in T Cells and Experimental Autoimmune Encephalomyelitis

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Ani Grigorian ◽  
Michael Demetriou
Keyword(s):  
T Cells ◽  
T Cell ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Demyelinating Disease ◽  
Cell Receptor ◽  
Protein Glycosylation ◽  
Autoimmune Encephalomyelitis ◽  
Glycosylation Pathway ◽  
Experimental Autoimmune ◽  
Surface Glycoproteins

Multiple sclerosis (MS) is an inflammatory demyelinating and neurodegenerative disease initiated by autoreactive T cells. Mgat5, a gene in the Asn (N-) linked protein glycosylation pathway, associates with MS severity and negatively regulates experimental autoimmune encephalomyelitis (EAE) and spontaneous inflammatory demyelination in mice. N-glycan branching by Mgat5 regulates interaction of surface glycoproteins with galectins, forming a molecular lattice that differentially controls the concentration of surface glycoproteins. T-cell receptor signaling, T-cell proliferation, TH1 differentiation, and CTLA-4 endocytosis are inhibited by Mgat5 branching. Non-T cells also contribute to MS pathogenesis and express abundant Mgat5 branched N-glycans. Here we explore whether Mgat5 deficiency in myelin-reactive T cells is sufficient to promote demyelinating disease. Adoptive transfer of myelin-reactive Mgat5−/− T cells into Mgat5+/+ versus Mgat5−/− recipients revealed more severe EAE in the latter, suggesting that Mgat5 branching deficiency in recipient naive T cells and/or non-T cells contribute to disease pathogenesis.

scholarly journals Ribonucleotide Reductase of Shrimp White Spot Syndrome Virus (WSSV): Expression and Enzymatic Activity in a Baculovirus/Insect Cell System and WSSV-Infected Shrimp

Virology ◽  
2002 ◽  
Vol 304 (2) ◽  
pp. 282-290 ◽  
Author(s):  
Shinn-Tsuen Lin ◽  
Yun-Shiang Chang ◽  
Han-Ching Wang ◽  
Huey-Fen Tzeng ◽  
Zee-Fen Chang ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Ribonucleotide Reductase ◽  
Insect Cell ◽  
White Spot Syndrome Virus ◽  
Cell System ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Effective production of oligomeric membrane proteins by EarlyBac-insect cell system

2021 ◽  
Author(s):  
Hiro Furukawa ◽  
Noriko Simorowski ◽  
Kevin Michalski
Keyword(s):  
Membrane Proteins ◽  
Insect Cell ◽  
Cell System

scholarly journals Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system

1999 ◽  
Vol 32 (1) ◽  
pp. 29-37 ◽  
Author(s):  
F. Bigi ◽  
O. Taboga ◽  
M.I. Romano ◽  
A. Alito ◽  
J.C. Fisanotti ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Insect Cell ◽  
Mycobacterium Smegmatis ◽  
Cell System
Sign in / Sign up

Export Citation Format

Share Document