Structural Characterization of Complex of Adenylation Domain and Carrier Protein by Using Pantetheine Cross-Linking Probe

2020 ◽  
Vol 15 (7) ◽  
pp. 1808-1812
Author(s):  
Akimasa Miyanaga ◽  
Shohei Kurihara ◽  
Taichi Chisuga ◽  
Fumitaka Kudo ◽  
Tadashi Eguchi
Keyword(s):  
Structural Characterization ◽  
Cross Linking ◽  
Carrier Protein ◽  
Adenylation Domain

Combining Electron Microscopy (EM) and Cross-Linking Mass Spectrometry (XL-MS) for Structural Characterization of Protein Complexes

2021 ◽  
pp. 217-232
Author(s):  
Lucía Quintana-Gallardo ◽  
Moisés Maestro-López ◽  
Jaime Martín-Benito ◽  
Miguel Marcilla ◽  
Daniel Rutz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Mass Spectrometry ◽  
Structural Characterization ◽  
Protein Complexes ◽  
Cross Linking

scholarly journals Carrier Protein-Free Chemo-Enzymatic Synthesis of Arylomycin A2 and Structural Characterization of the Cytochrome P450 AryC

2021 ◽  
Author(s):  
Tobias A. M. Gulder ◽  
Hülya Aldemir ◽  
Shuangjie Shu ◽  
Francoise Schaefers ◽  
Hanna Hong ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Structural Characterization ◽  
Enzymatic Synthesis ◽  
Carrier Protein

Physicochemical, functional and structural characterization of Mexican Oxalis tuberosa starch modified by cross-linking

2019 ◽  
Vol 13 (4) ◽  
pp. 2862-2870
Author(s):  
Lucila Concepción Núñez-Bretón ◽  
Liliana Catalina Cruz-Rodríguez ◽  
María Luisa Tzompole-Colohua ◽  
Jaime Jiménez-Guzmán ◽  
María de Jesús Perea-Flores ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Cross Linking ◽  
Oxalis Tuberosa

scholarly journals Thermodynamic and structural characterization of 2′-nitrogen-modified RNA duplexes

2004 ◽  
Vol 32 (11) ◽  
pp. 3446-3455 ◽  
Author(s):  
John W. Pham ◽  
Ishwar Radhakrishnan ◽  
Erik J. Sontheimer
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectroscopy ◽  
Nucleic Acids ◽  
Chemical Modification ◽  
Structural Characterization ◽  
Cross Linking ◽  
Base Pairing ◽  
Alkyl Groups ◽  
Rna Duplexes

Abstract 2′-aminonucleosides are commonly used as sites of post-synthetic chemical modification within nucleic acids. As part of a larger cross-linking strategy, we appended alkyl groups onto the N2′ position of 2′-amino-modified RNAs via 2′-ureido and 2′-amido linkages. We have characterized the thermodynamics of 2′-amino, 2′-alkylamido and 2′-alkylureido-modified RNA duplexes and show that 2′-ureido-modified RNAs are significantly more stable than analogous 2′-amido-modified RNAs. Using NMR spectroscopy and NMR-based molecular modeling of 2′-modified RNA duplexes, we examined the effects that 2′-nitrogen modifications have on RNA helices. Our data suggest that the 2′-ureido group forms a specific intra-nucleoside interaction that cannot occur within 2′-amido-modified helices. These results indicate that 2′-ureido modifications are superior to analogous 2′-amido ones for applications that require stable base pairing.

Structural Characterization of Phospholamban in Cardiac Sarcoplasmic Reticulum Membranes by Cross-Linking

1989 ◽  
Vol 8 (2) ◽  
pp. 95-106 ◽  
Author(s):  
Ellen F. Young ◽  
Martha J. McKee ◽  
Donald G. Ferguson ◽  
Evangelia G. Kranias
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Structural Characterization ◽  
Cross Linking ◽  
Cardiac Sarcoplasmic Reticulum

scholarly journals Large scale purification and structural characterization of squalene and sterol carrier protein.

1981 ◽  
Vol 256 (4) ◽  
pp. 1867-1873 ◽  
Author(s):  
M.E. Dempsey ◽  
K.E. McCoy ◽  
H.N. Baker ◽  
A. Dimitriadou-Vafiadou ◽  
T. Lorsbach ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Large Scale ◽  
Carrier Protein ◽  
Sterol Carrier Protein
Sign in / Sign up

Export Citation Format

Share Document