scholarly journals Click Chemistry: Mechanistic Insights into the Role of Amines Using Single-Molecule Spectroscopy

ACS Catalysis ◽  
2017 ◽  
Vol 7 (12) ◽  
pp. 8487-8492 ◽  
Author(s):  
Bowen Wang ◽  
Anabel E. Lanterna ◽  
Juan C. Scaiano
Keyword(s):  
Click Chemistry ◽  
Single Molecule ◽  
Single Molecule Spectroscopy

scholarly journals Stability of a Nonequilibrium Biochemical Cycle Revealed by Single-Molecule Spectroscopy

2021 ◽  
Author(s):  
Saurabh Talele ◽  
John T King
Keyword(s):  
Entropy Production ◽  
Single Molecule ◽  
Nonequilibrium Dynamics ◽  
Single Molecule Spectroscopy ◽  
Reaction Cycle ◽  
Central Interest ◽  
Thermodynamic Driving Force ◽  
Reaction Cycles ◽  
Thermal Stability Of

Biological machinery relies on nonequilibrium dynamics to maintain stable directional fluxes through complex reaction cycles. In stabilizing the reaction cycle, the role of microscopic irreversibility of elementary transitions, and the accompanying entropy production, is of central interest. Here, we use multidimensional single-molecule spectroscopy to demonstrate that the reaction cycle of bacteriorhodopsin is coupled through both reversible and irreversible transitions, with directionality of trans-membrane H+ transport being ensured by the entropy production of irreversible transitions. We observe that thermal destabilization of the process is the result of diminishing thermodynamic driving force for irreversible transitions, leading to an exponentially increasing variance of flux through the transitions. We show that the thermal stability of the reaction cycle can be predicted from the Gibbs-Helmholtz relation.

From the molecule to the mole: improving heterogeneous copper catalyzed click chemistry using single molecule spectroscopy

2017 ◽  
Vol 53 (2) ◽  
pp. 328-331 ◽  
Author(s):  
Bowen Wang ◽  
Javier Durantini ◽  
Matthew R. Decan ◽  
Jun Nie ◽  
Anabel E. Lanterna ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Single Molecule ◽  
Single Molecule Spectroscopy

scholarly journals Role of Denatured-State Properties in Chaperonin Action Probed by Single-Molecule Spectroscopy

2014 ◽  
Vol 107 (12) ◽  
pp. 2891-2902 ◽  
Author(s):  
Hagen Hofmann ◽  
Frank Hillger ◽  
Cyrille Delley ◽  
Armin Hoffmann ◽  
Shawn H. Pfeil ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Denatured State

Role of rare sites in single molecule spectroscopy measurements of spectral diffusion

2001 ◽  
Vol 114 (23) ◽  
pp. 10479-10485 ◽  
Author(s):  
Douglas S. English ◽  
Elizabeth J. Harbron ◽  
Paul F. Barbara
Keyword(s):  
Single Molecule ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy

On the role of spectral diffusion in single-molecule spectroscopy

2000 ◽  
Vol 42 (3) ◽  
pp. 477-480 ◽  
Author(s):  
V. V. Palm ◽  
K. K. Rebane ◽  
A. Zabrodskii
Keyword(s):  
Single Molecule ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy

Role of missing carotenoid in reducing the fluorescence of single monomeric photosystem II core complexes

2017 ◽  
Vol 19 (20) ◽  
pp. 13189-13194 ◽  
Author(s):  
Sepideh Skandary ◽  
Frank Müh ◽  
Imran Ashraf ◽  
Mohamed Ibrahim ◽  
Michael Metzger ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Single Molecule ◽  
Single Molecule Spectroscopy

The fluorescence of monomeric photosystem II core complexes of the cyanobacterium T. elongatus, originating from redissolved crystals, is investigated by using single-molecule spectroscopy.

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