scholarly journals The Solvent-Exposed Fe–S D-Cluster Contributes to Oxygen-Resistance in Desulfovibrio vulgaris Ni–Fe Carbon Monoxide Dehydrogenase

ACS Catalysis ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 7328-7335
Author(s):  
Elizabeth C. Wittenborn ◽  
Chloé Guendon ◽  
Mériem Merrouch ◽  
Martino Benvenuti ◽  
Vincent Fourmond ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Carbon Monoxide Dehydrogenase ◽  
Desulfovibrio Vulgaris

scholarly journals Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Elizabeth C Wittenborn ◽  
Mériem Merrouch ◽  
Chie Ueda ◽  
Laura Fradale ◽  
Christophe Léger ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Carbon Fixation ◽  
Conformational Dynamics ◽  
Oxidative Degradation ◽  
Carbon Monoxide Dehydrogenase ◽  
Cysteine Residue ◽  
Desulfovibrio Vulgaris ◽  
Cluster Assembly ◽  
X Ray Crystallography ◽  
Reduction Of Co2

The C-cluster of the enzyme carbon monoxide dehydrogenase (CODH) is a structurally distinctive Ni-Fe-S cluster employed to catalyze the reduction of CO2 to CO as part of the Wood-Ljungdahl carbon fixation pathway. Using X-ray crystallography, we have observed unprecedented conformational dynamics in the C-cluster of the CODH from Desulfovibrio vulgaris, providing the first view of an oxidized state of the cluster. Combined with supporting spectroscopic data, our structures reveal that this novel, oxidized cluster arrangement plays a role in avoiding irreversible oxidative degradation at the C-cluster. Furthermore, mutagenesis of a conserved cysteine residue that binds the C-cluster in the oxidized state but not in the reduced state suggests that the oxidized conformation could be important for proper cluster assembly, in particular Ni incorporation. Together, these results lay a foundation for future investigations of C-cluster activation and assembly, and contribute to an emerging paradigm of metallocluster plasticity.

scholarly journals The Carbon Monoxide Dehydrogenase from Desulfovibrio vulgaris

2015 ◽  
Vol 1847 (12) ◽  
pp. 1574-1583 ◽  
Author(s):  
Jessica Hadj-Saïd ◽  
Maria-Eirini Pandelia ◽  
Christophe Léger ◽  
Vincent Fourmond ◽  
Sébastien Dementin
Keyword(s):  
Carbon Monoxide ◽  
Carbon Monoxide Dehydrogenase ◽  
Desulfovibrio Vulgaris
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