Controlling the Membrane Perturbation by Tuning Charge Variable Cholate-Based Macromolecules

2022 ◽  
Author(s):  
Subhasish Sahoo ◽  
Bibhas Hazra ◽  
Pradip K. Tarafdar ◽  
Priyadarsi De
Keyword(s):  
Membrane Perturbation

scholarly journals Bordetella pertussis Lipid A Glucosamine Modification Confers Resistance to Cationic Antimicrobial Peptides and Increases Resistance to Outer Membrane Perturbation

2014 ◽  
Vol 58 (8) ◽  
pp. 4931-4934 ◽  
Author(s):  
Nita R. Shah ◽  
Robert E. W. Hancock ◽  
Rachel C. Fernandez
Keyword(s):  
Immune System ◽  
Antimicrobial Peptides ◽  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Lipid A ◽  
Whooping Cough ◽  
Human Immune System ◽  
Cationic Antimicrobial Peptides ◽  
Content Type ◽  
Membrane Perturbation

ABSTRACTBordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic antimicrobial peptides (CAMPs). We show modification of the lipid A region of LPS with glucosamine increased resistance to numerous CAMPs, including LL-37. Furthermore, we demonstrate that this glucosamine modification increased resistance to outer membrane perturbation.

Membrane perturbation by mastoparan 7 elicits a broad alteration in lipid composition of L1210 cells

2000 ◽  
Vol 1484 (2-3) ◽  
pp. 151-162 ◽  
Author(s):  
Heung Soon Park ◽  
Sang Yoon Lee ◽  
Young Hwan Kim ◽  
Jin Young Kim ◽  
Soo Jae Lee ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Membrane Perturbation ◽  
L1210 Cells

scholarly journals The toxic effect of Vu-Defr, a defensin from Vigna unguiculata seeds, on Leishmania amazonensis is associated with reactive oxygen species production, mitochondrial dysfunction, and plasma membrane perturbation

2018 ◽  
Vol 64 (7) ◽  
pp. 455-464 ◽  
Author(s):  
Géssika Silva Souza ◽  
Lais Pessanha de Carvalho ◽  
Edésio José Tenório de Melo ◽  
Valdirene Moreira Gomes ◽  
André de Oliveira Carvalho
Keyword(s):  
Reactive Oxygen Species ◽  
Mitochondrial Membrane ◽  
Biological Activities ◽  
Reactive Oxygen ◽  
Leishmania Amazonensis ◽  
New Drugs ◽  
Oxygen Species ◽  
Membrane Perturbation ◽  
Plant Defensins

Plant defensins are plant antimicrobial peptides that present diverse biological activities in vitro, including the elimination of Leishmania amazonensis. Plant defensins are considered promising candidates for the development of new drugs. This protozoan genus has great epidemiological importance and the mechanism behind the protozoan death by defensins is unknown, thus, we chose L. amazonensis for this study. The aim of the work was to analyze the possible toxic mechanisms of Vu-Defr against L. amazonensis. For analyses, the antimicrobial assay was repeated as previously described, and after 24 h, an aliquot of the culture was tested for viability, membrane perturbation, mitochondrial membrane potential, reactive oxygen species (ROS) and nitric oxide (NO) inductions. The results of these analyses indicated that after interaction with L. amazonensis, the Vu-Defr causes elimination of promastigotes from culture, membrane perturbation, mitochondrial membrane collapse, and ROS induction. Our analysis demonstrated that NO is not produced after Vu-Defr and L. amazonensis interaction. In conclusion, our work strives to help to fill the gap relating to effects caused by plant defensins on protozoan and thus better understand the mechanism of action of this peptide against L. amazonensis.

scholarly journals PLA2s-like membrane perturbation mechanism: extracting the most of crystallography data

2016 ◽  
Vol 72 (a1) ◽  
pp. s226-s226 ◽  
Author(s):  
Rafael J. Borges ◽  
Ney Lemke ◽  
Massimo Sammito ◽  
Claudia Millán ◽  
Isabel Usón ◽  
...  
Keyword(s):  
Membrane Perturbation

scholarly journals Membrane Perturbation Induced by Interfacially Adsorbed Peptides

2004 ◽  
Vol 86 (6) ◽  
pp. 3607-3619 ◽  
Author(s):  
Assaf Zemel ◽  
Avinoam Ben-Shaul ◽  
Sylvio May
Keyword(s):  
Membrane Perturbation

Localization of lipophosphonoglycan in membranes of Acanthamoeba by using specific antibodies

1981 ◽  
Vol 1 (4) ◽  
pp. 358-369
Author(s):  
C F Bailey ◽  
B Bowers
Keyword(s):  
Protein A ◽  
Contractile Vacuole ◽  
Membrane Perturbation ◽  
Cytoplasmic Vacuoles ◽  
Cytoplasmic Surface ◽  
Antibody Method ◽  
Membrane Compartment ◽  
Vacuolar Membranes ◽  
Indirect Immunofluorescent ◽  
Unlabeled Antibody

Antibodies against two electrophoretically distinct forms of lipophosphonoglycan (LPG) were produced in rabbits. Antibody specificity was demonstrated by the coupled antibody 125I-protein A assay (Adair et al., J. Cell Biol. 79:281-285, 1978). Indirect immunofluorescent labeling of intact Acanthamoeba showed that antibodies to both LPG components had the same uniform distribution on the cell surface. Both antibodies also bound to the cytoplasmic surface of isolated phagosomes. The location of LPG in other membranes of the amoeba was demonstrated on sections by the unlabeled antibody method. Although LPG was absent from the nuclear membrane, virtually all of the internal vacuole membranes were labeled, including the contractile vacuole. Antibodies directed against LPG were utilized to label lipophosphonoglycan in the plasma membrane of living amoebae. Labeled membrane was internalized and then localized by immunofluorescence in cytoplasmic vacuoles within 10 min of incubation. Although these results are evidence for exchange between plasma and cytoplasmic vacuolar membranes, the contractile vacuole remained unlabeled and can be considered, therefore, a separate membrane compartment. Concanavalin A also was bound and internalized by the amoeba, but electron microscopy showed that this label caused pronounced membrane perturbation, limiting its usefulness as a membrane marker in this system.

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