Fluorescent Probe Based on a Water-Soluble Conjugated Polymer: From Construction to Ultra-Low Concentration Biomarker Non-invasive Detection

2021 ◽  
Author(s):  
Mi Zhou ◽  
Zejian He ◽  
Ping Chen ◽  
Na Wu ◽  
Liangliang Zhu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Conjugated Polymer ◽  
Water Soluble ◽  
Low Concentration ◽  
Non Invasive

A specific fluorescent probe for fast detection and cellular imaging of cysteine based on a water-soluble conjugated polymer combined with copper(II)

Talanta ◽  
2019 ◽  
Vol 198 ◽  
pp. 128-136 ◽  
Author(s):  
Lihua Liu ◽  
Qiang Zhang ◽  
Jing Wang ◽  
Linlin Zhao ◽  
Lixia Liu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Conjugated Polymer ◽  
Cellular Imaging ◽  
Water Soluble ◽  
Fast Detection

Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  
Keyword(s):  
Microwave Heating ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Heating Process ◽  
X Ray ◽  
Doped Carbon Dots ◽  
Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models

2021 ◽  
pp. 109371
Author(s):  
Prasad M. Sonawane ◽  
Tesla Yudhistira ◽  
Mahesh B. Halle ◽  
Arkaprava Roychaudhury ◽  
Yunsu Kim ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Live Cells

Novel Water-Soluble Cyclodextrin-Based Conjugated Polymer for Selective Host–Guest Interactions of Cationic Surfactant CTAB and Reverse FRET with Rhodamine B Tagged Adamantyl Guest

2016 ◽  
Vol 49 (15) ◽  
pp. 5587-5598 ◽  
Author(s):  
Atul Kumar Dwivedi ◽  
Ravinder Singh ◽  
Ashutosh Singh ◽  
Kung-Hwa Wei ◽  
Chu-Ya Wu ◽  
...  
Keyword(s):  
Cationic Surfactant ◽  
Rhodamine B ◽  
Conjugated Polymer ◽  
Water Soluble

The synthesis of water-soluble phosphate pillar[5]arenes functionalized graphene as a fluorescent probe for sensitive detection of paraquat

Talanta ◽  
2019 ◽  
Vol 195 ◽  
pp. 472-479 ◽  
Author(s):  
Xiaoping Tan ◽  
Yue Wu ◽  
Sha Yu ◽  
Tingying Zhang ◽  
Hexiang Tian ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Sensitive Detection ◽  
Soluble Phosphate ◽  
Functionalized Graphene
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