Polymer Domains Control Diffusion in Protein–Polymer Conjugate Biosensors

2020 ◽  
Vol 2 (11) ◽  
pp. 4481-4492
Author(s):  
Justin M. Paloni ◽  
Bradley D. Olsen
Keyword(s):  
Protein Polymer ◽  
Polymer Conjugate ◽  
Control Diffusion

Site-specific in situ growth of a cyclized protein-polymer conjugate with improved stability and tumor retention

Biomaterials ◽  
2015 ◽  
Vol 47 ◽  
pp. 13-19 ◽  
Author(s):  
Jin Hu ◽  
Wenguo Zhao ◽  
Yan Gao ◽  
Mengmeng Sun ◽  
Yen Wei ◽  
...  
Keyword(s):  
In Situ Growth ◽  
Site Specific ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
Improved Stability ◽  
Tumor Retention

Nanoscale Proteinosomes Fabricated by Self-Assembly of a Supramolecular Protein–Polymer Conjugate

2017 ◽  
Vol 28 (2) ◽  
pp. 636-641 ◽  
Author(s):  
Guangda Han ◽  
Jin-Tao Wang ◽  
Xiaotian Ji ◽  
Li Liu ◽  
Hanying Zhao
Keyword(s):  
Self Assembly ◽  
Protein Polymer ◽  
Polymer Conjugate

scholarly journals Transforming protein-polymer conjugate purification by tuning protein solubility

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Stefanie L. Baker ◽  
Aravinda Munasinghe ◽  
Bibifatima Kaupbayeva ◽  
Nin Rebecca Kang ◽  
Marie Certiat ◽  
...  
Keyword(s):  
Ammonium Sulfate ◽  
Protein Solubility ◽  
Single Species ◽  
Salt Solutions ◽  
Dynamic Simulations ◽  
Salting Out ◽  
Polymer Conjugates ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
The Impact

Abstract Almost all commercial proteins are purified using ammonium sulfate precipitation. Protein-polymer conjugates are synthesized from pure starting materials, and the struggle to separate conjugates from polymer, native protein, and from isomers has vexed scientists for decades. We have discovered that covalent polymer attachment has a transformational effect on protein solubility in salt solutions. Here, protein-polymer conjugates with a variety of polymers, grafting densities, and polymer lengths are generated using atom transfer radical polymerization. Charged polymers increase conjugate solubility in ammonium sulfate and completely prevent precipitation even at 100% saturation. Atomistic molecular dynamic simulations show the impact is driven by an anti-polyelectrolyte effect from zwitterionic polymers. Uncharged polymers exhibit polymer length-dependent decreased solubility. The differences in salting-out are then used to simply purify mixtures of conjugates and native proteins into single species. Increasing protein solubility in salt solutions through polymer conjugation could lead to many new applications of protein-polymer conjugates.

Effect of Polymer Chain Density on Protein–Polymer Conjugate Conformation

2019 ◽  
Vol 20 (5) ◽  
pp. 1944-1955 ◽  
Author(s):  
Daniela Russo ◽  
Andrea de Angelis ◽  
Christopher. J. Garvey ◽  
Frederick R. Wurm ◽  
Marie-Sousai Appavou ◽  
...  
Keyword(s):  
Polymer Chain ◽  
Protein Polymer ◽  
Polymer Conjugate

Gold nanoparticle–protein conjugate dually-responsive to pH and temperature for modulation of enzyme activity

2019 ◽  
Vol 7 (20) ◽  
pp. 3260-3267
Author(s):  
Ya Sun ◽  
Zhenhua Li ◽  
Jingxian Wu ◽  
Zhiqiang Wang ◽  
Yishi Dong ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Enzymatic Activity ◽  
Gold Nanoparticle ◽  
Full Range ◽  
Protein Polymer ◽  
Temperature Conditions ◽  
Dual Responsive ◽  
Polymer Conjugate ◽  
Protein Conjugate

The enzymatic activity of the dual-responsive gold nanoparticle–protein–polymer conjugate can be modulated almost in a full range under different pH and temperature conditions.

Insight into Protein–Polymer Conjugate Relaxation Dynamics: The Importance of Polymer Grafting

2020 ◽  
Vol 20 (6) ◽  
pp. 1900410 ◽  
Author(s):  
Daniela Russo ◽  
Chiara Pelosi ◽  
Frederik R. Wurm ◽  
Bernhard Frick ◽  
Jacques Ollivier ◽  
...  
Keyword(s):  
Polymer Grafting ◽  
Relaxation Dynamics ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
Insight Into

scholarly journals Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly

2014 ◽  
Vol 5 (17) ◽  
pp. 4884-4895 ◽  
Author(s):  
Dongsook Chang ◽  
Christopher N. Lam ◽  
Shengchang Tang ◽  
Bradley D. Olsen
Keyword(s):  
Phase Transitions ◽  
Block Copolymer ◽  
Self Assembly ◽  
Polymer Chemistry ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
Cubic Phases ◽  
Polymer Interactions ◽  
Nanostructure Control ◽  
Polymer Block

Changing polymer chemistry in protein–polymer conjugate block copolymers results in the formation of previously unobserved cubic phases and changes in protein–polymer interactions that create large shifts in phase transitions, providing a powerful tool for nanostructure control.

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