scholarly journals Polymer−Peptide Conjugates Convert Amyloid into Protein Nanobundles through Fragmentation and Lateral Association

2019 ◽  
Vol 3 (2) ◽  
pp. 937-945 ◽  
Author(s):  
John W. Smith ◽  
Xing Jiang ◽  
Hyosung An ◽  
Alexander M. Barclay ◽  
Giuseppe Licari ◽  
...  
Keyword(s):  
Peptide Conjugates ◽  
Lateral Association

68Ga-labeled HBED-CC Variant of uPAR Targeting Peptide AE105 Compared with 68Ga-NODAGA-AE105

2019 ◽  
Vol 18 (9) ◽  
pp. 1289-1294 ◽  
Author(s):  
Kusum Vats ◽  
Rohit Sharma ◽  
Haladhar D. Sarma ◽  
Drishty Satpati ◽  
Ashutosh Dash
Keyword(s):  
Solid Phase ◽  
Evaluation Studies ◽  
Radiochemical Yield ◽  
In Vivo Evaluation ◽  
Peptide Conjugates ◽  
Biodistribution Studies ◽  
Target Organs ◽  
U87mg Tumor

Aims: The urokinase Plasminogen Activator Receptors (uPAR) over-expressed on tumor cells and their invasive microenvironment are clinically significant molecular targets for cancer research. uPARexpressing cancerous lesions can be suitably identified and their progression can be monitored with radiolabeled uPAR targeted imaging probes. Hence this study aimed at preparing and evaluating two 68Ga-labeled AE105 peptide conjugates, 68Ga-NODAGA-AE105 and 68Ga-HBED-CC-AE105 as uPAR PET-probes. Method: The peptide conjugates, HBED-CC-AE105-NH2 and NODAGA-AE105-NH2 were manually synthesized by standard Fmoc solid phase strategy and subsequently radiolabeled with 68Ga eluted from a commercial 68Ge/68Ga generator. In vitro cell studies for the two radiotracers were performed with uPAR positive U87MG cells. Biodistribution studies were carried out in mouse xenografts with the subcutaneously induced U87MG tumor. Results: The two radiotracers, 68Ga-NODAGA-AE105 and 68Ga-HBED-CC-AE105 that were prepared in >95% radiochemical yield and >96% radiochemical purity, exhibited excellent in vitro stability. In vivo evaluation studies revealed higher uptake of 68Ga-HBED-CC-AE105 in U87MG tumor as compared to 68Ga-NODAGAAE105; however, increased lipophilicity of 68Ga-HBED-CC-AE105 resulted in slower clearance from blood and other non-target organs. The uPAR specificity of the two radiotracers was ascertained by significant (p<0.05) reduction in the tumor uptake with a co-injected blocking dose of unlabeled AE-105 peptide. Conclusion: Amongst the two radiotracers studied, the neutral 68Ga-NODAGA-AE105 with more hydrophilic chelator exhibited faster clearance from non-target organs. The conjugation of HBED-CC chelator (less hydrophilic) resulted in negatively charged 68Ga-HBED-CC-AE105 which was observed to have high retention in blood that decreased target to non-target ratios.

Peptide conjugates of lactoferricin analogues and antimicrobials—Design, chemical synthesis, and evaluation of antimicrobial activity and mammalian cytotoxicity

Peptides ◽  
2019 ◽  
Vol 117 ◽  
pp. 170079 ◽  
Author(s):  
Natalia Ptaszyńska ◽  
Katarzyna Olkiewicz ◽  
Joanna Okońska ◽  
Katarzyna Gucwa ◽  
Anna Łęgowska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Synthesis ◽  
Peptide Conjugates

scholarly journals Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study

2016 ◽  
Vol 113 (40) ◽  
pp. 11152-11157 ◽  
Author(s):  
Carlos G. Lopez ◽  
Oliva Saldanha ◽  
Klaus Huber ◽  
Sarah Köster
Keyword(s):  
Light Scattering ◽  
Quantitative Model ◽  
Radius Of Gyration ◽  
Time Resolved ◽  
Intermediate Filament Proteins ◽  
Hierarchical Assembly ◽  
Lateral Association ◽  
Cytoskeletal Filament ◽  
Longitudinal Association ◽  
Scattering Study

Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60–600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.

Synthesis of Nucleic Acid-Peptide Conjugates Targeted to Proteins

2011 ◽  
Vol 51 (8-9) ◽  
pp. 876-884 ◽  
Author(s):  
Anne Erben ◽  
Oliver Seitz
Keyword(s):  
Nucleic Acid ◽  
Peptide Conjugates

High-Efficiency Intracellular Magnetic Labeling with Novel Superparamagnetic-Tat Peptide Conjugates

1999 ◽  
Vol 10 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Lee Josephson ◽  
Ching-Hsuan Tung ◽  
Anna Moore ◽  
Ralph Weissleder
Keyword(s):  
High Efficiency ◽  
Tat Peptide ◽  
Peptide Conjugates ◽  
Magnetic Labeling

Enhanced Strand Invasion by Peptide Nucleic Acid−Peptide Conjugates†

Biochemistry ◽  
2002 ◽  
Vol 41 (37) ◽  
pp. 11118-11125 ◽  
Author(s):  
Kunihiro Kaihatsu ◽  
Dwaine A. Braasch ◽  
Ahmet Cansizoglu ◽  
David R. Corey
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Conjugates ◽  
Strand Invasion
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