Quantum Dots as Förster Resonance Energy Transfer Acceptors of Lanthanides in Time-Resolved Bioassays

2018 ◽  
Vol 1 (6) ◽  
pp. 3006-3014 ◽  
Author(s):  
Sebastián A. Díaz ◽  
Guillermo Lasarte-Aragones ◽  
Robert G. Lowery ◽  
Aniket ◽  
James N. Vranish ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Time Resolved ◽  
Förster Resonance

scholarly journals Time-Resolved Analysis of a Highly Sensitive Förster Resonance Energy Transfer Immunoassay Using Terbium Complexes as Donors and Quantum Dots as Acceptors

2007 ◽  
Vol 2007 ◽  
pp. 1-6 ◽  
Author(s):  
Niko Hildebrandt ◽  
Loïc J. Charbonnière ◽  
Hans-Gerd Löhmannsröben
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Decay Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Luminescence Decay ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Time Resolved ◽  
Förster Resonance

CdSe/ZnS core/shell quantum dots (QDs) are used as efficient Förster Resonance Energy Transfer (FRET) acceptors in a time-resolved immunoassays with Tb complexes as donors providing a long-lived luminescence decay. A detailed decay time analysis of the FRET process is presented. QD FRET sensitization is evidenced by a more than 1000-fold increase of the QD luminescence decay time reaching ca. 0.5 milliseconds, the same value to which the Tb donor decay time is quenched due to FRET to the QD acceptors. The FRET system has an extremely large Förster radius of approx. 100 Å and more than 70% FRET efficiency with a mean donor-acceptor distance of ca. 84 Å, confirming the applied biotin-streptavidin binding system. Time-resolved measurement allows for suppression of short-lived emission due to background fluorescence and directly excited QDs. By this means a detection limit of 18 attomol QDs within the immunoassay is accomplished, an improvement of more than two orders of magnitude compared to commercial systems.

scholarly journals Förster Resonance Energy Transfer between Colloidal CuInS2/ZnS Quantum Dots and Dark Quenchers

2019 ◽  
Vol 124 (2) ◽  
pp. 1717-1731 ◽  
Author(s):  
Chenghui Xia ◽  
Wentao Wang ◽  
Liang Du ◽  
Freddy T. Rabouw ◽  
Dave J. van den Heuvel ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance

scholarly journals Förster Resonance Energy Transfer Distance Dependence from Upconverting Nanoparticles to Quantum Dots

2018 ◽  
Vol 122 (32) ◽  
pp. 18751-18758 ◽  
Author(s):  
Sonia Melle ◽  
Oscar G. Calderón ◽  
Marco Laurenti ◽  
Diego Mendez-Gonzalez ◽  
Ana Egatz-Gómez ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Transfer Distance ◽  
Distance Dependence ◽  
Förster Resonance

Lanthanides and Quantum Dots as Förster Resonance Energy Transfer Agents for Diagnostics and Cellular Imaging

2013 ◽  
Vol 53 (4) ◽  
pp. 1824-1838 ◽  
Author(s):  
Daniel Geißler ◽  
Stina Linden ◽  
Konstanze Liermann ◽  
K. David Wegner ◽  
Loïc J. Charbonnière ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cellular Imaging ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance ◽  
Transfer Agents

scholarly journals Förster Resonance Energy Transfer between Core/Shell Quantum Dots and Bacteriorhodopsin

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mark H. Griep ◽  
Eric M. Winder ◽  
Donald R. Lueking ◽  
Gregory A. Garrett ◽  
Shashi P. Karna ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Separation Distance ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Core Shell ◽  
Energy Transfer Mechanism ◽  
Förster Resonance

An energy transfer relationship between core-shell CdSe/ZnS quantum dots (QDs) and the optical protein bacteriorhodopsin (bR) is shown, demonstrating a distance-dependent energy transfer with 88.2% and 51.1% of the QD energy being transferred to the bR monomer at separation distances of 3.5 nm and 8.5 nm, respectively. Fluorescence lifetime measurements isolate nonradiative energy transfer, other than optical absorptive mechanisms, with the effective QD excited state lifetime reducing from 18.0 ns to 13.3 ns with bR integration, demonstrating the Förster resonance energy transfer contributes to 26.1% of the transferred QD energy at the 3.5 nm separation distance. The established direct energy transfer mechanism holds the potential to enhance the bR spectral range and sensitivity of energies that the protein can utilize, increasing its subsequent photocurrent generation, a significant potential expansion of the applicability of bR in solar cell, biosensing, biocomputing, optoelectronic, and imaging technologies.

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