Bioinspired Antimicrobial Nanodots with Amphiphilic and Zwitterionic-like Characteristics for Combating Multidrug-Resistant Bacteria and Biofilm Removal

2018 ◽  
Vol 1 (5) ◽  
pp. 2062-2068 ◽  
Author(s):  
Hesheng Victor Xu ◽  
Xin Ting Zheng ◽  
Chao Wang ◽  
Yanli Zhao ◽  
Yen Nee Tan
Keyword(s):  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Biofilm Removal

scholarly journals Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4974 ◽  
Author(s):  
Carla Dias ◽  
Anabela Borges ◽  
Diana Oliveira ◽  
Antonio Martinez-Murcia ◽  
Maria José Saavedra ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Animal Health ◽  
Multidrug Resistant ◽  
Contact Angles ◽  
Resistant Bacteria ◽  
Wild Animals ◽  
Genetic Determinants ◽  
Multidrug Resistant Bacteria ◽  
Biofilm Removal

BackgroundThe “One Health” concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance.MethodsThe purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacterspp.,Klebsiella pneumoniae,Pseudomonas fluorescensandShewanella putrefaciens) isolated from wild animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles.ResultsThe susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for β-lactamases were detected inK. pneumoniae(TEM, SHV, OXA-aer) and one inP. fluorescens(OXA-aer).K. pneumoniaewas the microorganism that carried more β-lactamase genes and it was the most proficient biofilm producer, whileP. fluorescensdemonstrated the highest adhesion ability. Antibiotics at their MIC, 5 × MIC and 10 × MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 × MIC) againstK. pneumoniaebiofilms and with CIP (40% at 10 × MIC) againstP. fluorescensbiofilms.DiscussionThe results highlight wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence.

C-terminal Lysine-Linked Magainin 2 with Increased Activity Against Multidrug-Resistant Bacteria

2016 ◽  
Vol 23 (8) ◽  
pp. 738-747 ◽  
Author(s):  
Esteban N. Lorenzón ◽  
Norival A. Santos-Filho ◽  
Matheus A. S. Ramos ◽  
Tais M. Bauab ◽  
Ilana L. B. C. Camargo ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Increased Activity

scholarly journals Identification of Aminoglycoside Resistance Genes From Bacteria Isolated From Selected Municipal Drinking Water Distribution Systems in Southwestern Nigeria

2020 ◽  
Vol 41 (S1) ◽  
pp. s255-s255
Author(s):  
Ayodele T. Adesoji ◽  
Adeniyi A. Ogunjobi
Keyword(s):  
Drinking Water ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Southwestern Nigeria ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

Background: Multidrug-resistant bacteria can lead to treatment failure, resulting in infectious diseases being transferred through nonpotable water. Aminoglycosides are an important class of antibiotics that are abused in Nigeria. Few studies have investigated aminoglycoside-modifying genes (AMGs) that are likely responsible for resistance in Nigeria bacteria isolates. Therefore, we aimed to characterize AMGs from isolates in drinking water distribution systems (DWDS) in southwestern Nigeria. Methods: Multidrug-resistant bacteria (n = 181) that had been previously characterized by 16S rDNA sequencing and that were positive for resistance to at least 1 aminoglycoside antibiotic were selected from 6 treated and untreated water distribution systems. Strains were PCR genotyped for 3 AMGs: aph(3)c, ant(3)b and aph(6)-1dd. Results: Of 181 MDR bacteria tested, 69 (38.12%) were positive for at least 1 of the AMGs. The most common was ant(3)c (27.6%), followed by aph(3")c (18.23%). Both aph(3)c and ant(3")b were found in 7.73% of tested isolates, ant(3)b was most commonly found in Alcaligenes spp (50%). Furthermore, aph(3")c was most commonly detected in Proteus spp (50%). Other genera positive for AMGs included Acinetobacter, Aeromonas, Bordetella, Brevundimonas, Chromobacterium, Klebsiella, Leucobacter, Morganella, Pantoae, Proteus, Providencia, Psychrobacter, and Serratia. Conclusions: High occurrence of ant(3)c and aph(3)c among these bacteria call for urgent attention among public health workers because these genes can be easily disseminated to consumers if present on mobile genetic elements like plasmids, integrons, and transposons.Funding: NoneDisclosures: None

Sign in / Sign up

Export Citation Format

Share Document