Nanostructured TiN-Coated Electrodes for High-Sensitivity Noninvasive Characterization of in Vitro Tissue Models

2018 ◽  
Vol 1 (5) ◽  
pp. 2284-2293 ◽  
Author(s):  
Tobias Schmitz ◽  
Matthias Schweinlin ◽  
Robin T. Kollhoff ◽  
Lisa Engelhardt ◽  
Christian Lotz ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Noninvasive Characterization ◽  
Tissue Models ◽  
Coated Electrodes

Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽  
2016 ◽  
Vol 12 (5) ◽  
Author(s):  
Érika Cristina dos Santos ◽  
Camila Bruna de Lima ◽  
Kelly Annes ◽  
Marcella Pecora Milazzotto
Keyword(s):  
Culture Media ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Noninvasive Characterization ◽  
Vitro Production

Mechanical characterization of bioprinted in vitro soft tissue models

2013 ◽  
Vol 5 (4) ◽  
pp. 045010 ◽  
Author(s):  
Ting Zhang ◽  
Karen Chang Yan ◽  
Liliang Ouyang ◽  
Wei Sun
Keyword(s):  
Soft Tissue ◽  
Mechanical Characterization ◽  
Tissue Models

scholarly journals Synthesis, Cytotoxicity Assessment and Optical Properties Characterization of Colloidal GdPO4:Mn2+, Eu3+ for High Sensitivity Luminescent Nanothermometers Operating in the Physiological Temperature Range

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 421 ◽  
Author(s):  
Kamila Maciejewska ◽  
Blazej Poźniak ◽  
Marta Tikhomirov ◽  
Adrianna Kobylińska ◽  
Łukasz Marciniak
Keyword(s):  
Synthesis Method ◽  
High Sensitivity ◽  
Relative Sensitivity ◽  
Spectroscopic Properties ◽  
Physiological Temperature ◽  
Temperature Range ◽  
Cytotoxicity Studies ◽  
Cytotoxicity Assessment

Herein, a novel synthesis method of colloidal GdPO4:Mn2+,Eu3+ nanoparticles for luminescent nanothermometry is proposed. XRD, TEM, DLS, and zeta potential measurements confirmed the crystallographic purity and reproducible morphology of the obtained nanoparticles. The spectroscopic properties of GdPO4:Mn2+,Eu3+ with different amounts of Mn2+ and Eu3+ were analyzed in a physiological temperature range. It was found that GdPO4:1%Eu3+,10%Mn2+ nanoparticles revealed extraordinary performance for noncontact temperature sensing with relative sensitivity SR = 8.88%/°C at 32 °C. Furthermore, the biocompatibility and safety of GdPO4:15%Mn2+,1%Eu3+ was confirmed by cytotoxicity studies. These results indicated that colloidal GdPO4 doped with Mn2+ and Eu3+ is a very promising candidate as a luminescent nanothermometer for in vitro applications.

Engineered Microtissues for Real-Time Characterization of Cardiomyocyte Function

2013 ◽  
Author(s):  
Ariane C. C. van Spreeuwel ◽  
Noortje A. M. Bax ◽  
Jasper Foolen ◽  
M. A. Borochin ◽  
Daisy W. J. van der Schaft ◽  
...  
Keyword(s):  
Real Time ◽  
Cardiac Tissue ◽  
Mechanical Performance ◽  
Diagnostic Tools ◽  
Cardiac Physiology ◽  
Surrounding Matrix ◽  
Speed Up ◽  
Tissue Models

Engineered cardiac tissue models become increasingly important for understanding normal and disease cardiac physiology [1]. Where clinical diagnostic tools usually measure overall function of the heart, cardiac tissue models make it possible to focus on single CMs and their microenvironment. The use of in-vitro cardiac disease models can give more insight in the functionality changes of CMs during disease and thereby speed up the development of new therapies. Therefore, we aim to develop a model for healthy and diseased myocardium to study the effect of diseased microenvironments on the mechanical performance of CMs. The platform consists of 3D engineered microtissues with matrix, CMs and fibroblasts (FBs) on an array of polydimethylsiloxane (PDMS) microposts and allows for real-time characterization of CMs and their surrounding matrix. The design was adapted from Legant et. al. [2] and enables us to measure inhomogeneous tissue forces which may occur if not all cells contract equally. Here we focus on optimization and validation of the platform to measure contraction forces and gain insight in CM mechanical functioning.

Preparation and Characterization of Active Targeting Dual-Functionalized Liposome with Anti-Tumor Ability and Optimized Fluorescent Intensity

2020 ◽  
Vol 20 (9) ◽  
pp. 5305-5312
Author(s):  
Zian Wang ◽  
Yang Liu ◽  
Yifei Jiang ◽  
Peng Wang ◽  
Min Ji
Keyword(s):  
High Sensitivity ◽  
Cationic Liposome ◽  
Fluorescent Intensity ◽  
Photothermal Heating ◽  
Tumor Hyperthermia ◽  
Acidic Conditions ◽  
Cell Inhibition ◽  
Inhibition Ability

Although the preparation of Indocyanine Green (ICG) liposomes obtained stronger performance than free ICG. With increase in depth of tissue, ICG exhibits limited background (SBR) and blurs structure characteristics. In this research, a Stearylamine-Bearing cationic liposome was prepared for improved fluorescence performance (higher SBR and deeper imaging depth). In addition, the effect of ICG and lipid interactions was explored. Hyaluronic acid is subsequently modified on the liposomes for prolonging blood circulation time and active tumor targeting. In vitro study confirmed that the liposome (HA-ICG-SA-LP) was capable of reversing surface zeta potential under acidic conditions in the presence of HAase which might enhance cellular uptake. Additionally, the photothermal heating of liposomes was investigated. The MTT assay showed that the liposome has strong cancer cell inhibition ability. In summary, HA-ICG-SA-LP exhibited a great potential for high sensitivity imaging and tumor hyperthermia.

Ultrastructural identification of three types of sensory setae on copepod antennae

1995 ◽  
Vol 53 ◽  
pp. 956-957
Author(s):  
T. M. Weatherby ◽  
P.H. Lenz
Keyword(s):  
Phase Locking ◽  
Membrane Surface ◽  
Reaction Times ◽  
High Sensitivity ◽  
Structural Features ◽  
Calanoid Copepods ◽  
Marine Food Webs ◽  
Dendritic Membrane ◽  
Ultrastructural Characterization

Crustaceans, as well as other arthropods, are covered with sensory setae and hairs, including mechanoand chemosensory sensillae with a ciliary origin. Calanoid copepods are small planktonic crustaceans forming a major link in marine food webs. In conjunction with behavioral and physiological studies of the antennae of calanoids, we undertook the ultrastructural characterization of sensory setae on the antennae of Pleuromamma xiphias.Distal mechanoreceptive setae exhibit exceptional behavioral and physiological performance characteristics: high sensitivity (<10 nm displacements), fast reaction times (<1 msec latency) and phase locking to high frequencies (1-2 kHz). Unusual structural features of the mechanoreceptors are likely to be related to their physiological sensitivity. These features include a large number (up to 3000) of microtubules in each sensory cell dendrite, arising from or anchored to electron dense rods associated with the ciliary basal body microtubule doublets. The microtubules are arranged in a regular array, with bridges between and within rows. These bundles of microtubules extend far into each mechanoreceptive seta and terminate in a staggered fashion along the dendritic membrane, contacting a large membrane surface area and providing a large potential site of mechanotransduction.

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