Multifunctional Nanolabels Based on Polydopamine Nanospheres for Sensitive Alpha Fetoprotein Electrochemical Detection

2021 ◽  
Author(s):  
Yunli Wang ◽  
Yaxing Hong ◽  
Ming Wang ◽  
Yingchun Zhu
Keyword(s):  
Electrochemical Detection ◽  
Alpha Fetoprotein

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

Preparation of Fe3O4@PS/PDA-Au nanotubes for sensitive electrochemical detection of alpha-fetoprotein

2018 ◽  
Vol 814 ◽  
pp. 52-58 ◽  
Author(s):  
Fang Peng ◽  
Meilin Chu ◽  
Jiajia Sun ◽  
Yuhong Liu ◽  
Qicheng Zhang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Alpha Fetoprotein

Horseradish peroxidase functionalized gold nanorods as a label for sensitive electrochemical detection of alpha-fetoprotein antigen

2015 ◽  
Vol 491 ◽  
pp. 58-64 ◽  
Author(s):  
Jinjin Guo ◽  
Xiaowei Han ◽  
Junchun Wang ◽  
Junqing Zhao ◽  
Zilin Guo ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Electrochemical Detection ◽  
Gold Nanorods ◽  
Alpha Fetoprotein

Mixed Self-Assembled Aptamer and Newly Designed Zwitterionic Peptide as Antifouling Biosensing Interface for Electrochemical Detection of alpha-Fetoprotein

ACS Sensors ◽  
2017 ◽  
Vol 2 (4) ◽  
pp. 490-494 ◽  
Author(s):  
Min Cui ◽  
Yu Wang ◽  
Mingxia Jiao ◽  
Silambarasan Jayachandran ◽  
Yumin Wu ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Alpha Fetoprotein ◽  
Self Assembled

scholarly journals On the Electrochemical Detection of Alpha-Fetoprotein Using Aptamers: DNA Isothermal Amplification Strategies to Improve the Performance of Weak Aptamers

Biosensors ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 46
Author(s):  
Ramón Lorenzo-Gómez ◽  
Daniel González-Robles ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Dissociation Constant ◽  
Electrochemical Detection ◽  
Magnetic Beads ◽  
Rolling Circle Amplification ◽  
Binding Constants ◽  
Alpha Fetoprotein ◽  
Isothermal Amplification ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Tumor Biomarker ◽  
Rolling Circle

Affinity characterization is essential to develop reliable aptamers for tumor biomarker detection. For alpha-fetoprotein (AFP), a biomarker of hepatocellular carcinoma (HCC), two DNA aptamers were described with very different affinity. In this work, we estimate the dissociation constant of both of them by means of a direct assay on magnetic beads modified with AFP and electrochemical detection on carbon screen-printed electrodes (SPCE). Unlike previous works, both aptamers showed similar dissociation constant (Kd) values, in the subµM range. In order to improve the performance of these aptamers, we proposed the isothermal amplification of the aptamers by both terminal deoxynucleotidyl transferase (TdT) and rolling circle amplification (RCA). Both DNA amplifications improved the sensitivity and also the apparent binding constants from 713 nM to 189 nM for the short aptamer and from 526 nM to 32 nM for the long aptamer. This improvement depends on the true affinity of the binding pair, which ultimately limits the analytical usefulness.

Alpha-fetoprotein producing gastric cancer cells lack transcription factor AT-motif binding factor 1 (ATBF1)

2001 ◽  
Vol 120 (5) ◽  
pp. A31-A31
Author(s):  
H KATAOKA ◽  
T JOH ◽  
T OHSHIMA ◽  
Y ITOH ◽  
K SENOO ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Alpha Fetoprotein ◽  
Gastric Cancer Cells ◽  
Binding Factor
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