Bovine Serum Albumin-Encapsulated Ultrasmall Gold Nanoclusters for Photodynamic Therapy of Tumors

2021 ◽  
Author(s):  
Tianzi Geng ◽  
Lining Zhao ◽  
Di Wu ◽  
Hongxin Zhang ◽  
Xiaolong Zhao ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gold Nanoclusters

Tetra-sulfonate phthalocyanine zinc-bovine serum albumin conjugate-mediated photodynamic therapy of human glioma

2014 ◽  
Vol 29 (3) ◽  
pp. 378-385 ◽  
Author(s):  
Dianshuang Xu ◽  
Xiangyu Chen ◽  
Ke’en Chen ◽  
Yiru Peng ◽  
Yingxin Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Human Glioma ◽  
Bovine Serum Albumin Conjugate

scholarly journals Identifying Reducing and Capping Sites of Protein-Encapsulated Gold Nanoclusters

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1630 ◽  
Author(s):  
Yu-Chen Hsu ◽  
Mei-Jou Hung ◽  
Yi-An Chen ◽  
Tsu-Fan Wang ◽  
Ying-Ru Ou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Local Structure ◽  
Bovine Serum ◽  
Gold Nanoclusters ◽  
Cysteic Acid ◽  
Subsequent Growth ◽  
The Core

The reducing and capping sites along with their local structure impact photo properties of the red bovine serum albumin-capped Au nanocluster (BSA-AuNC), however, they are hard to identify. We developped a workflow and relevant techniques using mass spectrometry (MS) to identify the reducing and capping sites of BSA-AuNCs involved in their formation and fluorescence. Digestion without disulfide cleavages yielded an Au core fraction exhibiting red fluorescence and [AunSm] ion signals and a non-core fraction exhibiting neither of them. The core fraction was identified to mainly be comprised of peptides containing cysteine residues. The fluorescence and [AunSm] signals were quenched by tris(2-carboxyethyl)phosphine, confirming that disulfide groups were required for nanocluster stabilization and fluorescence. By MS sequencing, the disulfide pairs, C75–C91/C90–C101 in domain IA, C315–C360/C359–C368 in domain IIB, and C513–C558/C557–C566 in domain IIIB, were identified to be main capping sites of red AuNCs. Peptides containing oxidized cysteines (sulfinic or cysteic acid) were identified as reducing sites mainly in the non-core fraction, suggesting that disulfide cleavages by oxidization and conformational changes contributed to the subsequent growth of nanoclusters at nearby intact disulfide pairs. This is the first report on precise identification of the reducing and capping sites of BSA-AuNCs.

scholarly journals A Turn-On Fluorescent Sensor for Glutathione Based on Bovine Serum Albumin-Stabilized Gold Nanoclusters

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Qiu ◽  
Jianlin Huang ◽  
Li Jia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescent Sensor ◽  
Gold Nanoclusters ◽  
Copper Ion ◽  
Fluorescence Sensor ◽  
Gold Nanocluster ◽  
Experimental Conditions ◽  
Bovine Serum Albumin Molecule

A fluorescence sensor for the detection of glutathione based on the fluorescence recovering of the bovine serum albumin-stabilized gold nanoclusters is reported. This study indicates that glutathione restores the copper-ion-quenched fluorescence by coordinating the bound copper ion in the bovine serum albumin molecule used for stabilizing the gold nanocluster through its sulfydryl. Under the experimental conditions, the fluorescence response showed a linear relationship with the concentration of glutathione over the range from 10 µM to 400 µM. The fluorescence sensor successfully detected glutathione in commercial drug products.

Functionalized diphenylchlorins and bacteriochlorins: their synthesis and bioconjugation for targeted photodynamic therapy and tumour cell imaging

2000 ◽  
Vol 04 (07) ◽  
pp. 655-658 ◽  
Author(s):  
JONATHAN M. SUTTON ◽  
NELSON FERNANDEZ ◽  
ROSS W. BOYLE
Keyword(s):  
Photodynamic Therapy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Tumour Cell ◽  
Bovine Serum ◽  
Osmium Tetroxide ◽  
Cell Imaging ◽  
Important Group ◽  
Structure Activity ◽  
Targeted Photodynamic Therapy

A range of 5,15-diphenylporphyrins with symmetric and unsymmetric substitution patterns were subjected to osmium tetroxide-mediated dihydroxylations. The resulting chlorins and bacteriochlorins present an important group of compounds for studying structure-activity relationships of photodynamic sensitizers. The regioselectivity of the dihydroxylation of various 5,15-diphenylporphyrins with unsymmetrical substitution patterns was also examined. Both diphenylchlorin diol (DPC) and diphenylbacteriochlorin tetrol (DPBC) examples were converted into reactive isothiocyanates and conjugated with bovine serum albumin (BSA).

Ratiometric fluorescence determination of alkaline phosphatase activity based on dual emission of bovine serum albumin-stabilized gold nanoclusters and the inner filter effect

The Analyst ◽  
2021 ◽  
Author(s):  
Li Pu ◽  
Mengfan Xia ◽  
Pengyue Sun ◽  
Yaodong Zhang
Keyword(s):  
Alkaline Phosphatase ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Filter Effect

Ratiometric fluorescence assay of alkaline phosphatase based on dual emission of bovine serum albumin-templated gold nanoclusters and inner filter effect.

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