Photostable and Biocompatible Luminescent Thiol-Terminated Organosilica Nanoparticles with Embedded Au(I)–Thiolate Complexes for Fluorescent Microscopic Imaging

2021 ◽  
Author(s):  
Chihiro Mochizuki ◽  
Junna Nakamura ◽  
Michihiro Nakamura
Keyword(s):  
Microscopic Imaging ◽  
Thiolate Complexes

Highly Fluorescent Au25-xAgx Nanoclusters Protected with Poly(ethylene glycol) - and Zwitterion-Modified Thiolate Ligands

2018 ◽  
Author(s):  
Dinesh Mishra ◽  
Sisi Wang ◽  
Zhicheng Jin ◽  
Eric Lochner ◽  
Hedi Mattoussi
Keyword(s):  
Quantum Yield ◽  
Near Infrared ◽  
Small Diameter ◽  
Binding Energies ◽  
Thiolate Ligands ◽  
Nir Emission ◽  
Thiolate Complexes ◽  
Long Lifetime ◽  
Poly Ethylene

<p>We describe the growth and characterization of highly fluorescing, near-infrared-emitting nanoclusters made of bimetallic Au<sub>25-x</sub>Ag<sub>x</sub> cores, prepared using various monothiol-appended hydrophobic and hydrophilic ligands. The reaction uses well-defined triphenylphosphine-protected Au<sub>11</sub> clusters (as precursors), which are reacted with Ag(I)-thiolate complexes. The prepared nanoclusters are small (diameter < 2nm, as characterized by TEM) with emission peak at 760 nm and long lifetime (~12 µs). The quantum yield measured for these materials was 0.3 - 0.4 depending on the ligand. XPS measurements show the presence of both metal atoms in the core, with measured binding energies that agree with reported values for nanocluster materials. The NIR emission combined with high quantum yield, small size and ease of surface functionalization afforded by the coating, make these materials suitable to implement investigations that address fundamental questions and potentially useful for biological sensing and imaging applications.<br></p>

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

Microscopic imaging of extended tissue volumes

2004 ◽  
Vol 31 (12) ◽  
pp. 902-905 ◽  
Author(s):  
Ian LeGrice ◽  
Greg Sands ◽  
Darren Hooks ◽  
Dane Gerneke ◽  
Bruce Smaill
Keyword(s):  
Microscopic Imaging

Syndiospecific Polymerization of Methyl Methacrylate Catalyzed by Lanthanoid Thiolate Complexes Bearing a Hexamethylphosphoric Triamide Ligand

1996 ◽  
Vol 29 (24) ◽  
pp. 8014-8016 ◽  
Author(s):  
Yuushou Nakayama ◽  
Tetsuya Shibahara ◽  
Hiroki Fukumoto ◽  
Akira Nakamura ◽  
Kazushi Mashima
Keyword(s):  
Methyl Methacrylate ◽  
Hexamethylphosphoric Triamide ◽  
Thiolate Complexes

Computational study on the mechanism of hydroboration of CO2 catalysed by POCOP pincer nickel thiolate complexes: concerted catalysis and hydride transfer by a shuttle

2021 ◽  
Vol 50 (8) ◽  
pp. 2903-2914
Author(s):  
Nana Ma ◽  
Chenhao Tu ◽  
Qingli Xu ◽  
Wenyue Guo ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Computational Study ◽  
Hydride Transfer ◽  
Dft Computations ◽  
Thiolate Complexes

The concerted catalysis and hydride shuttle mechanism for pincer nickel thiolate catalysed hydroboration of CO2 was established by DFT computations.

Polymerization of acrylonitrile catalyzed by thiolate complexes of divalent and trivalent lanthanoids

1999 ◽  
Vol 48 (6) ◽  
pp. 502-504 ◽  
Author(s):  
Yuushou Nakayama ◽  
Hiroki Fukumoto ◽  
Tetsuya Shibahara ◽  
Akira Nakamura ◽  
Kazushi Mashima
Keyword(s):  
Thiolate Complexes
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