Magnetic Separation of Antibodies with High Binding Capacity by Site-Directed Immobilization of Protein A-Domains to Bare Iron Oxide Nanoparticles

2021 ◽  
Author(s):  
Yasmin Kaveh-Baghbaderani ◽  
Raphaela Allgayer ◽  
Sebastian Patrick Schwaminger ◽  
Paula Fraga-García ◽  
Sonja Berensmeier
Keyword(s):  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Binding Capacity ◽  
Protein A ◽  
Oxide Nanoparticles ◽  
High Binding ◽  
A Domains

Magnetic separation of polymer hybrid iron oxide nanoparticles triggered by temperature

2006 ◽  
pp. 2765 ◽  
Author(s):  
Yabin Sun ◽  
Xiaobin Ding ◽  
Zhaohui Zheng ◽  
Xu Cheng ◽  
Xinhua Hu ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Polymer Hybrid

Synthesis and Characterization of L-Lysin Coated Iron Oxide Nanoparticles as Appropriate Choices for Cell Immobilization and Magnetic Separation

2019 ◽  
Vol 9 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Mohammad Javad Raee ◽  
Alireza Ebrahiminezhad ◽  
Mohammad Bagher Ghoshoon ◽  
Ahmad Gholami ◽  
Younes Ghasemi
Keyword(s):  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Cell Separation ◽  
Culture Media ◽  
Cell Immobilization ◽  
Good Alternative ◽  
Oxide Nanoparticles ◽  
Bacterial Cells ◽  
Separation Processes

Introduction:Cell separation is one of the important steps of purification in downstream processes. Some separation techniques such as centrifugation and filtration are expensive and would affect cell viability. Magnetic separation can be a good alternative for laboratory and industrial cell separation processes.Methods:For this purpose, L-lysine coated Iron Oxide Nanoparticles (IONs) were synthesized and used for magnetic separation of Escherichia coli as the most applied microbial cell in biotechnological processes.Results:IONs have successfully decorated the bacterial cells and cells were completely separated by applying an external magnetic field.Conclusion:This study showed that coating of E. coli cells with IONs could help to isolate cells from culture media without using expensive instruments.

scholarly journals Construction of a device for magnetic separation of superparamagnetic iron oxide nanoparticles

2015 ◽  
Vol 1 (1) ◽  
pp. 306-309 ◽  
Author(s):  
Kerstin Kläser ◽  
Matthias Graeser ◽  
Dirk Steinhagen ◽  
Kerstin Luedtke-Buzug
Keyword(s):  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Iron Oxide Particles ◽  
Huge Impact ◽  
Magnetic Resonance Imaging Mri ◽  
Particle Imaging

AbstractSuspensions of iron oxide particles, so called ferrofluids, are successfully used in various technical, biochemical and medical applications. For example they find use in the area of sensor engineering, magnetic resonance imaging (MRI) and especially magnetic particle imaging (MPI). MPI is a new tomographic imaging technique that determines the spatial distribution of superparamagnetic iron oxide nanoparticles (SPIONs). Besides a very high spatial and temporal resolution MPI provides quantitative realtime imageing. The nanoparticles cause a magnetization change that can be measured. As the particle size distribution has a huge impact on the magnetization behavior is an important parameter for optimization. While synthesizing, SPIONs particles with various dimensions are formed what necessitates a systematically separation by size. For this purpose a construction of a simple device for magnetic separation of SPIONs has been developed. First attemps of separation show the potential of this method.

scholarly journals Kinetics of Aggregation and Magnetic Separation of Multicore Iron Oxide Nanoparticles: Effect of the Grafted Layer Thickness

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 623 ◽  
Author(s):  
Hinda Ezzaier ◽  
Jéssica Marins ◽  
Cyrille Claudet ◽  
Gauvin Hemery ◽  
Olivier Sandre ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Layer Thickness ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Dipolar Coupling ◽  
Molecular Layer ◽  
Adsorbed Layer ◽  
Capture Efficiency ◽  
Oxide Nanoparticles ◽  
Induced Aggregation

In this work, we have studied field-induced aggregation and magnetic separation—realized in a microfluidic channel equipped with a single magnetizable micropillar—of multicore iron oxide nanoparticles (IONPs) also called “nanoflowers” of an average size of 27 ± 4 nm and covered by either a citrate or polyethylene (PEG) monolayer having a thickness of 0.2–1 nm and 3.4–7.8 nm, respectively. The thickness of the adsorbed molecular layer is shown to strongly affect the magnetic dipolar coupling parameter because thicker molecular layers result in larger separation distances between nanoparticle metal oxide multicores thus decreasing dipolar magnetic forces between them. This simple geometrical constraint effect leads to the following important features related to the aggregation and magnetic separation processes: (a) Thinner citrate layer on the IONP surface promotes faster and stronger field-induced aggregation resulting in longer and thicker bulk needle-like aggregates as compared to those obtained with a thicker PEG layer; (b) A stronger aggregation of citrated IONPs leads to an enhanced retention capacity of these IONPs by a magnetized micropillar during magnetic separation. However, the capture efficiency Λ at the beginning of the magnetic separation seems to be almost independent of the adsorbed layer thickness. This is explained by the fact that only a small portion of nanoparticles composes bulk aggregates, while the main part of nanoparticles forms chains whose capture efficiency is independent of the adsorbed layer thickness but depends solely on the Mason number Ma. More precisely, the capture efficiency shows a power law trend Λ ∝ M a − n , with n ≈ 1.4–1.7 at 300 < Ma < 104, in agreement with a new theoretical model. Besides these fundamental issues, the current work shows that the multicore IONPs with a size of about 30 nm have a good potential for use in biomedical sensor applications where an efficient low-field magnetic separation is required. In these applications, the nanoparticle surface design should be carried out in a close feedback with the magnetic separation study in order to find a compromise between biological functionalities of the adsorbed molecular layer and magnetic separation efficiency.

Shaping iron oxide nanocrystals for magnetic separation applications

Nanoscale ◽  
2018 ◽  
Vol 10 (43) ◽  
pp. 20462-20467 ◽  
Author(s):  
Martín Testa-Anta ◽  
Sara Liébana-Viñas ◽  
Beatriz Rivas-Murias ◽  
Benito Rodríguez González ◽  
Michael Farle ◽  
...  
Keyword(s):  
Magnetic Susceptibility ◽  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Magnetophoretic Mobility

The large magnetophoretic mobility stemming from the large magnetic susceptibility and the very small coercivity of octapod-shaped iron oxide nanoparticles improve their capability for magnetic separation.

scholarly journals Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin

2021 ◽  
Vol 14 (5) ◽  
pp. 405
Author(s):  
Chiara Turrina ◽  
Sonja Berensmeier ◽  
Sebastian P. Schwaminger
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Drug Loading ◽  
Binding Potential ◽  
Oxide Nanoparticles ◽  
Release Process ◽  
Free Peptide ◽  
High Binding ◽  
Drug Delivery Carrier

New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on Escherichia coli show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.

scholarly journals Biosynthesized Iron Oxide Nanoparticles from Petroselinum crispum Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features

Toxics ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 123
Author(s):  
Sabry M. El-Bahr ◽  
Amal M. Elbakery ◽  
Nashwa El-Gazzar ◽  
Aziza A. Amin ◽  
Saad Al-Sultan ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Lead Acetate ◽  
Leaf Extract ◽  
Binding Capacity ◽  
Albino Rats ◽  
Petroselinum Crispum ◽  
Oxide Nanoparticles ◽  
Tem Analysis ◽  
Leukocytic Infiltration

This study aimed to investigate the ameliorative effects of iron oxide nanoparticles (IONPs) prepared from leaf extract of Petroselinum crispum compared to those prepared using a chemical method in lead-acetate-induced anemic rats. Twenty rats were divided into four groups (five rats each). Throughout the experimental period (8 weeks), the rats in group 1 were not given any therapy. The rats in groups 2, 3 and 4 were given 400 ppm lead acetate orally for 2 weeks to make them anemic. Following that, these rats were either left untreated, given 27 ppm of chemical IONPs orally or given 27 ppm of natural IONPs orally for the remaining 6 weeks of the experiment. TEM analysis indicated that the chemically and naturally prepared IONPs had sizes of 6.22–9.7  and 64–68 nm, respectively. Serum ferritin and iron concentrations were reduced, whereas the total iron-binding capacity (TIBC), ALT, AST, urea and creatinine were significantly increased in the non-treated lead-acetate-induced anemic rats compared to those of the control. In addition, congestion, hemorrhage, necrosis, vacuolation and leukocytic infiltration in the kidneys, liver and spleen were observed in non-treated lead-acetate-induced anemic rats compared to the control. The effects of lead acetate were mitigated by IONPs, particularly the natural one. In conclusion, IONPs produced from Petroselinum crispum leaf extract can be used as an efficient and safe therapy in lead-acetate-induced anemic rats.

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