Remodeling of Cellular Surfaces via Fast Disulfide–Thiol Exchange To Regulate Cell Behaviors

2019 ◽  
Vol 11 (51) ◽  
pp. 47750-47761
Author(s):  
Lianghua He ◽  
Huaiji Wang ◽  
Yi Han ◽  
Kun Wang ◽  
Haiqing Dong ◽  
...  
Keyword(s):  
Cell Behaviors ◽  
Thiol Exchange

Rapid build-up of high-throughput screening microarrays with biochemistry gradients via light-induced thiol–ene “click” chemistry

2021 ◽  
Vol 9 (13) ◽  
pp. 3032-3037
Author(s):  
Hongye Hao ◽  
Junjie Huang ◽  
Ping Liu ◽  
Yunfan Xue ◽  
Jing Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Behaviors

Microarrays with biochemistry gradients were rapidly fabricated via light-induced thiol–ene “click” chemistry and showed great applicability in cell behaviors screening.

scholarly journals ANLN promotes carcinogenesis in oral cancer by regulating the PI3K/mTOR signaling pathway

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Bing Wang ◽  
Xiao-li Zhang ◽  
Chen-xi Li ◽  
Ning-ning Liu ◽  
Min Hu ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Signaling Pathway ◽  
Cell Cycle Progression ◽  
Mtor Signaling ◽  
Mtor Signaling Pathway ◽  
Cell Behaviors ◽  
Rnai Technology ◽  
Tumor Tissues ◽  
Pi3k Signaling Pathway

Abstract Background Oral cancer is a malignant disease that threatenshuman life and greatly reducespatientquality of life. ANLN was reported to promote the progression of cancer. This study aims to investigate the role of ANLNin oral cancer and the underlying molecular mechanism. Methods ANLN expression was downregulated by RNAi technology. The effect of ANLN on cell behaviors, including proliferation, cell cycle progression, invasion, and apoptosis, was detected. Western blotting analysis was used to explore the mechanism by whichANLN functions in oral cancer. Results Data from TCGA database showed that ANLN was expressed at significantly higher levels in tumor tissues thanin normal control tissues. Patients with higher ANLN expression exhibitedshorter survivaltimes. ANLN was alsoabundantly expressedin the cancer cell lines CAL27 and HN30. When ANLN was knocked down in CAL27 and HN30 cells, cell proliferation and colony formation weredecreased. The cell invasion ability was also inhibited. However, the cell apoptosis rate was increased. In addition, the levels of critical members of the PI3K signaling pathway, includingPI3K, mTOR, Akt, and PDK-1, were significantlyreducedafter ANLN was knocked down in CAL27 cells. Conclusions ANLN contributes to oral cancerprogressionand affects activation ofthe PI3K/mTOR signaling pathway. This study providesa new potential targetfor drug development and treatment in oral cancer.

Regulation of Osteoblast-Like Cell Behaviors on Hydroxyapatite by Electrical Polarization

2007 ◽  
Vol 361-363 ◽  
pp. 1055-1058 ◽  
Author(s):  
Miho Nakamura ◽  
Akiko Nagai ◽  
Natalie Ohashi ◽  
Yumi Tanaka ◽  
Yasutaka Sekijima ◽  
...  
Keyword(s):  
Cell Movement ◽  
Stress Fiber ◽  
Fiber Formation ◽  
Wound Healing Assay ◽  
Cell Behaviors ◽  
Stress Fiber Formation ◽  
Cytoskeleton Reorganization ◽  
Osteoblast Adhesion ◽  
As Stress

The osteoblast adhesion to the substrates are recognized to play a fundamental role in osteoconduction process. The purpose of this study was to evaluate the in vitro behavior of osteoblasts cultured on polarized hydroxyapatite (HA), having the enhanced osteobonding abilities. Osteoblast-like cells were seeded onto the polarized HA and investigated the adhesion and motility. The polarization had no effects on the percentage of the number of the spreaded cells against all the adhered cells, but had significant effects on the elongation of adhered cells from fluorescent observation and on the cell motility showed by the wound healing assay. The charges induced on the HA surface accelerated the cytoskeleton reorganization of the adhered cells cultured on HA specimens. The acceleration was emerged as the cells shape, actin filament pattern such as stress fiber formation, and the prolongation of the cell movement distances.

scholarly journals Cell behaviors during endoderm morphogenesis in the mouse gastrula

2008 ◽  
Vol 319 (2) ◽  
pp. 504
Author(s):  
Manuel Viotti ◽  
Gloria S. Kwon ◽  
Kat Hadjantonakis
Keyword(s):  
Cell Behaviors

scholarly journals Data-driven modeling reveals cell behaviors controlling self-organization during Myxococcus xanthus development

2017 ◽  
Vol 114 (23) ◽  
pp. E4592-E4601 ◽  
Author(s):  
Christopher R. Cotter ◽  
Heinz-Bernd Schüttler ◽  
Oleg A. Igoshin ◽  
Lawrence J. Shimkets
Keyword(s):  
Cell Tracking ◽  
Myxococcus Xanthus ◽  
Cell Movement ◽  
Modeling Method ◽  
Data Driven ◽  
Self Organization ◽  
Emergent Properties ◽  
Developmental Cycle ◽  
Fluorescent Cell ◽  
Cell Behaviors

Collective cell movement is critical to the emergent properties of many multicellular systems, including microbial self-organization in biofilms, embryogenesis, wound healing, and cancer metastasis. However, even the best-studied systems lack a complete picture of how diverse physical and chemical cues act upon individual cells to ensure coordinated multicellular behavior. Known for its social developmental cycle, the bacterium Myxococcus xanthus uses coordinated movement to generate three-dimensional aggregates called fruiting bodies. Despite extensive progress in identifying genes controlling fruiting body development, cell behaviors and cell–cell communication mechanisms that mediate aggregation are largely unknown. We developed an approach to examine emergent behaviors that couples fluorescent cell tracking with data-driven models. A unique feature of this approach is the ability to identify cell behaviors affecting the observed aggregation dynamics without full knowledge of the underlying biological mechanisms. The fluorescent cell tracking revealed large deviations in the behavior of individual cells. Our modeling method indicated that decreased cell motility inside the aggregates, a biased walk toward aggregate centroids, and alignment among neighboring cells in a radial direction to the nearest aggregate are behaviors that enhance aggregation dynamics. Our modeling method also revealed that aggregation is generally robust to perturbations in these behaviors and identified possible compensatory mechanisms. The resulting approach of directly combining behavior quantification with data-driven simulations can be applied to more complex systems of collective cell movement without prior knowledge of the cellular machinery and behavioral cues.

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