Aptamer-Based Fluorescent Biosensing of Adenosine Triphosphate and Cytochrome c via Aggregation-Induced Emission Enhancement on Novel Label-Free DNA-Capped Silver Nanoclusters/Graphene Oxide Nanohybrids

2019 ◽  
Vol 11 (49) ◽  
pp. 46077-46089 ◽  
Author(s):  
Mojtaba Shamsipur ◽  
Karam Molaei ◽  
Fatemeh Molaabasi ◽  
Saman Hosseinkhani ◽  
Avat Taherpour ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cytochrome C ◽  
Adenosine Triphosphate ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Aggregation Induced Emission ◽  
Free Dna ◽  
Emission Enhancement ◽  
Fluorescent Biosensing

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

Stable silver nanoclusters with aggregation-induced emission enhancement for detection of aluminum ion

2019 ◽  
Vol 278 ◽  
pp. 181-189 ◽  
Author(s):  
Xianhu Liu ◽  
Congying Shao ◽  
Tiedan Chen ◽  
Zhanjun He ◽  
Gangfeng Du
Keyword(s):  
Silver Nanoclusters ◽  
Aggregation Induced Emission ◽  
Aluminum Ion ◽  
Emission Enhancement

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing

2014 ◽  
Vol 60 ◽  
pp. 161-166 ◽  
Author(s):  
Zhao Zhang ◽  
Liqiang Luo ◽  
Guifang Chen ◽  
Yaping Ding ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene
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