Self-Immolative Fluorescent and Raman Probe for Real-Time Imaging and Quantification of γ-Glutamyl Transpeptidase in Living Cells

2019 ◽  
Vol 11 (31) ◽  
pp. 27529-27535 ◽  
Author(s):  
Yang Zhang ◽  
Gengwu Zhang ◽  
Peng Yang ◽  
Basem Moosa ◽  
Niveen M. Khashab
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Real Time Imaging ◽  
Glutamyl Transpeptidase ◽  
Raman Probe

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

scholarly journals Reactive oxygen species-triggered off-on fluorescence donor for imaging hydrogen sulfide delivery in living cells

2019 ◽  
Vol 10 (33) ◽  
pp. 7690-7694 ◽  
Author(s):  
Yiming Hu ◽  
Xiaoyi Li ◽  
Yu Fang ◽  
Wen Shi ◽  
Xiaohua Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Sulfide ◽  
Real Time ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
The Real ◽  
Real Time Imaging

A reactive oxygen species-triggered off-on fluorescence H2S donor is develop for the real-time imaging of H2S delivery and the cytoprotection against the hazardous oxidative environment.

Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays

2015 ◽  
Vol 10 (9) ◽  
pp. 791-795 ◽  
Author(s):  
C. Laborde ◽  
F. Pittino ◽  
H. A. Verhoeven ◽  
S. G. Lemay ◽  
L. Selmi ◽  
...  
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Real Time Imaging

Molecular Beacons for Sensitive Gene Detection in Living Cells

2003 ◽  
Author(s):  
Andrew Tsurkas ◽  
Gang Bao
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Molecular Beacon ◽  
Point Mutations ◽  
Living Cells ◽  
Molecular Beacons ◽  
High Signal ◽  
Gene Detection ◽  
Real Time Imaging ◽  
Background Ratio

Real-time imaging of gene expression in living cells has the potential to significantly impact clinical and laboratory studies of cancer, including cancer diagnosis and analysis. Molecular beacons (MBs) provide a simple and promising tool for the detection of target mRNA as tumor markers due to their high signal-to-background ratio, and their improved specificity in detecting point mutations. However, the harsh intracellular environment does limit the sensitivity of MB-based gene detection. Specifically, MBs bound to target mRNAs cannot be distinguished from those degraded by nucleases, or opened due to non-specific interactions. To overcome this difficulty, we have developed a novel dual FRET molecular beacons approach in which a pair of molecular beacons, one with a donor fluorophore and a second with an acceptor fluorophore, hybridize to adjacent regions on the same target resulting in fluorescence resonance energy transfer (FRET). The detection of a FRET signal leads to a substantially increased signal-to-background ratio compared with that in single molecular beacon assays and enables discrimination between fluorescence due to specific probe/target hybridization and a variety of false-positive events. We have performed systematic in-solution and cellular studies of dual FRET molecular beacon and demonstrated that this new approach allows for real-time imaging of gene expression in living cells.

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