scholarly journals Naked Liquid Marbles: A Robust Three-Dimensional Low-Volume Cell-Culturing System

2019 ◽  
Vol 11 (10) ◽  
pp. 9814-9823 ◽  
Author(s):  
Mo Chen ◽  
Megha P. Shah ◽  
Todd B. Shelper ◽  
Lynn Nazareth ◽  
Matthew Barker ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Liquid Marbles ◽  
Cell Culturing ◽  
Low Volume ◽  
Volume Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Cell culturing in a three-dimensional matrix affects the localization and properties of plasma membrane cholesterol

2009 ◽  
Vol 33 (10) ◽  
pp. 1079-1086 ◽  
Author(s):  
Nadezhda Stefanova ◽  
Galya Staneva ◽  
Diana Petkova ◽  
Teodora Lupanova ◽  
Roumen Pankov ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Three Dimensional ◽  
Membrane Cholesterol ◽  
Dimensional Matrix ◽  
Cell Culturing ◽  
Plasma Membrane Cholesterol

Low-volume-change, dendrite-free lithium metal anodes enabled by lithophilic 3D matrix with LiF-enriched surface

2019 ◽  
Vol 7 (11) ◽  
pp. 6090-6098 ◽  
Author(s):  
Yangyang Feng ◽  
Chaofan Zhang ◽  
Bing Li ◽  
Shizhao Xiong ◽  
Jiangxuan Song
Keyword(s):  
Synergistic Effect ◽  
Volume Change ◽  
Three Dimensional ◽  
Lithium Metal ◽  
Metal Anode ◽  
3D Matrix ◽  
Lithium Metal Anodes ◽  
Li Metal Anode ◽  
Low Volume ◽  
Novel Concept

We report a novel concept for a stable Li metal anode via the synergistic effect of a three-dimensional skeleton and stable artificial SEI.

scholarly journals Correction: Scaffold-free three-dimensional cell culturing using magnetic levitation

2018 ◽  
Vol 6 (7) ◽  
pp. 1996-1996
Author(s):  
Esra Türker ◽  
Nida Demirçak ◽  
Ahu Arslan-Yildiz
Keyword(s):  
Magnetic Levitation ◽  
Three Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

Correction for ‘Scaffold-free three-dimensional cell culturing using magnetic levitation’ by Esra Türker et al., Biomater. Sci., 2018, DOI: 10.1039/c8bm00122g.

Challenges and opportunities to integrate the oldest and newest manufacturing processes: metal casting and additive manufacturing

2020 ◽  
Vol 26 (6) ◽  
pp. 1145-1154 ◽  
Author(s):  
Paul Lynch ◽  
C.R. Hasbrouck ◽  
Joseph Wilck ◽  
Michael Kay ◽  
Guha Manogharan
Keyword(s):  
Supply Chain ◽  
Additive Manufacturing ◽  
Supply Chains ◽  
Service Providers ◽  
Three Dimensional ◽  
Metal Casting ◽  
Content Type ◽  
Digital Ecosystem ◽  
Volume Production ◽  
Low Volume

Purpose This paper aims to investigate the current state, technological challenges, economic opportunities and future directions in the growing “indirect” hybrid manufacturing ecosystem, which integrates traditional metal casting with the production of tooling via additive manufacturing (AM) process including three-dimensional sand printing (3DSP) and printed wax patterns. Design/methodology/approach A survey was conducted among 100 participants from foundries and AM service providers across the USA to understand the current adoption of AM in metal casting as a function of engineering specifications, production demand, volume and cost metrics. In addition, current technological and logistical challenges that are encountered by the foundries are identified to gather insight into the future direction of this evolving supply chain. Findings One of the major findings from this study is that hard tooling costs (i.e. patterns/core boxes) are the greatest challenge in low volume production for foundries. Hence, AM and 3DSP offer the greatest cost-benefit for these low volume production runs as it does not require the need for hard tooling to produce much higher profit premium castings. It is evident that there are major opportunities for the casting supply chain to benefit from an advanced digital ecosystem that seamlessly integrates AM and 3DSP into foundry operations. The critical challenges for adoption of 3DSP in current foundry operations are categorized into as follows: capital cost of the equipment, which cannot be justified due to limited demand for 3DSP molds/cores by casting buyers, transportation of 3DSP molds and cores, access to 3DSP, limited knowledge of 3DSP, limitations in current design tools to integrate 3DSP design principles and long lead times to acquire 3DSP molds/cores. Practical implications Based on the findings of this study, indirect hybrid metal AM supply chains, i.e. 3DSP metal casting supply chains is proposed, as 3DSP replaces traditional mold-making in the sand casting process flow, no/limited additional costs and resources would be required for qualification and certification of the cast parts made from three-dimensional printed sand molds. Access to 3DSP resources can be addressed by establishing a robust 3DSP metal casting supply chain, which will also enable existing foundries to rapidly acquire new 3DSP-related knowledge. Originality/value This original survey from 100 small and medium enterprises including foundries and AM service providers suggests that establishing 3DSP hubs around original equipment manufacturers as a shared resource to produce molds and cores would be beneficial. This provides traditional foundries means to continue mass production of castings using existing hard tooling while integrating 3DSP for new complex low volume parts, replacement parts, legacy parts and prototyping.

scholarly journals STUDY ON NONLINEAR PAVEMENT RESPONSES OF LOW VOLUME ROADWAYS SUBJECT TO MULTIPLE WHEEL LOADS / NETIESINĖS KELIO DANGOS PRIKLAUSOMYBĖS NUO MAŽO INTENSYVUMO KELIŲ, VEIKIAMŲ DAUGKARTINĖMIS RATŲ APKROVOMIS, TYRIMAS

2011 ◽  
Vol 17 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Minkwan Kim ◽  
Joo Hyoung Lee
Keyword(s):  
Finite Element ◽  
Numerical Study ◽  
Asphalt Pavement ◽  
Three Dimensional ◽  
Nonlinear Behavior ◽  
Element Model ◽  
Element Analysis ◽  
Nonlinear Stress ◽  
Low Volume ◽  
Stress Dependent

This paper describes numerical analyses on low volume roads (LVRs) using a nonlinear three-dimensional (3D) finite element model (FEM). Various pavement scenarios are analyzed to investigate the effects of pavement layer thicknesses, traffic loads, and material properties on pavement responses, such as surface deflection and subgrade strain. Each scenario incorporates a different combination of wheel/axle configurations and pavement geomaterial properties to analyze the nonlinear behavior of thinly surfaced asphalt pavement. In this numerical study, nonlinear stress-dependent models are employed in the base and subgrade layers to properly characterize pavement geomaterial behavior. Finite element analysis results are then described in terms of the effects of the asphalt pavement thickness, wheel/axle configurations, and geomaterial properties on critical pavement responses. Conclusions are drawn by the comparison of the nonlinear pavement responses in the base and subgrade in association with the effects of multiple wheel/axle load interactions. Santrauka Straipsnyje aprašoma skaitinė mažo intensyvumo kelių analizė, taikant netiesinį—erdvinį baigtinių elementų modelį. Skirtingi dangų paviršiaus variantai analizuojami siekiant ištirti, kokiąįtaką kelio dangos elgsenai, t. y. poslinkiams ir kelio pagrindo deformacijoms, turi dangų sluoksnių storiai, eismo apkrovos ir medžiagų savybės. Kiekvienas kelio dangos variantas turi skirtingas ratų arba ašies ir geometrinių savybių formas, kad būtų galima išanalizuoti netiesinę plonos asfalto dangos paviršiaus elgseną. Šioje skaitinėje analizėje nagrinėjami netiesiniai įtempių modeliai, kurie buvo taikomi pagrindo sluoksniams, siekiant tinkamai apibūdinti geometrinę kelio dangos elgseną. Baigtinių elementų analizės rezultatai toliau nagrinėjami atsižvelgiant į asfalto dangos storį ar ašies formą ir geometrines savybes, priklausomai nuo kritinės kelio dangos būklės. Išvados buvo gautos lyginant netiesines kelių dangos priklausomybes pagrindo sluoksnyje, atsižvelgiant į jų sąveiką su daugkartine ratų apkrova.

scholarly journals Capillary origami: superhydrophobic ribbon surfaces and liquid marbles

2011 ◽  
Vol 2 ◽  
pp. 145-151 ◽  
Author(s):  
Glen McHale ◽  
Michael I Newton ◽  
Neil J Shirtcliffe ◽  
Nicasio R Geraldi
Keyword(s):  
Thin Sheet ◽  
Three Dimensional ◽  
Rigid Surface ◽  
Rigid Substrate ◽  
Elastic Substrate ◽  
Interfacial Tensions ◽  
Liquid Marbles ◽  
Interfacial Energies ◽  
Induced Folding ◽  
Solid Liquid

In the wetting of a solid by a liquid it is often assumed that the substrate is rigid. However, for an elastic substrate the rigidity depends on the cube of its thickness and so reduces rapidly as the substrate becomes thinner as it approaches becoming a thin sheet. In such circumstances, it has been shown that the capillary forces caused by a contacting droplet of a liquid can shape the solid rather than the solid shaping the liquid. A substrate can be bent and folded as a (pinned) droplet evaporates or even instantaneously and spontaneously wrapped on contact with a droplet. When this effect is used to create three dimensional shapes from initially flat sheets, the effect is called capillary origami or droplet wrapping. In this work, we consider how the conditions for the spontaneous, capillary induced, folding of a thin ribbon substrate might be altered by a rigid surface structure that, for a rigid substrate, would be expected to create Cassie–Baxter and Wenzel effects. For smooth thin substrates, droplet wrapping can occur for all liquids, including those for which the Young’s law contact angle (defined by the interfacial tensions) is greater than 90° and which would therefore normally be considered relatively hydrophobic. However, consideration of the balance between bending and interfacial energies suggests that the tendency for droplet wrapping can be suppressed for some liquids by providing the flexible solid surface with a rigid topographic structure. In general, it is known that when a liquid interacts with such a structure it can either fully penetrate the structure (the Wenzel case) or it can bridge between the asperities of the structure (the Cassie–Baxter case). In this report, we show theoretically that droplet wrapping should occur with both types of solid–liquid contact. We also derive a condition for the transition between the Cassie–Baxter and Wenzel type droplet wrapping and relate it to the same transition condition known to apply to superhydrophobic surfaces. The results are given for both droplets being wrapped by thin ribbons and for solid grains encapsulating droplets to form liquid marbles.

Cryostructuring of polymer systems. 47. Preparation of wide porous gelatin-based cryostructurates in sterilizing organic media and assessment of the suitability of thus formed matrices as spongy scaffolds for 3D cell culturing

e-Polymers ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 175-186 ◽  
Author(s):  
Vladimir I. Lozinsky ◽  
Valentina K. Kulakova ◽  
Roman V. Ivanov ◽  
Alexander Yu. Petrenko ◽  
Olena Yu. Rogulska ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Human Adipose Tissue ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Organic Media ◽  
Ethanolic Solution ◽  
Polymer Systems ◽  
Cell Culturing ◽  
Ethanol Medium ◽  
The Matrix ◽  
Cell Growth And Differentiation

AbstractNew gelatin-based cryostructurates have been elaborated and tested as scaffolds for three-dimensional (3D) cell culturing. Scaffold preparation included dissolution of Type A gelatin in dimethylsulfoxide, freezing of such solution, cryoextraction of crystalline phase with cold ethanol, cross-linking of gelatin with carbodiimide in ethanol medium, treatment of the matrix with ethanolic solution of Tris and tanning of the matrix with formaldehyde dissolved in ethanol. The use of organic media during all the preparation stages ensured the sterility of the scaffolds. The matrices thus prepared were seeded with human adipose tissue multipotent mesenchymal stromal cells to confirm the biocompatibility of scaffolds and their possibility to provide necessary environment for the cell growth and differentiation. The cells attached onto the surface of the pore walls, proliferated and differentiated into osteogenic and adipogenic lineages. These results demonstrate that gelatin-based cryostructurates prepared in the sterility ensuring organic media can be used as scaffolds for tissue engineering purposes.

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