Collagen Functionalized With Graphene Oxide Enhanced Biomimetic Mineralization and in Situ Bone Defect Repair

2018 ◽  
Vol 10 (50) ◽  
pp. 44080-44091 ◽  
Author(s):  
Chuchao Zhou ◽  
Shaokai Liu ◽  
Jialun Li ◽  
Ke Guo ◽  
Quan Yuan ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bone Defect ◽  
Biomimetic Mineralization ◽  
Bone Defect Repair ◽  
Defect Repair

An in situ phototriggered-imine-crosslink composite hydrogel for bone defect repair

2016 ◽  
Vol 4 (5) ◽  
pp. 973-981 ◽  
Author(s):  
Jieyuan Zhang ◽  
Yunlong Yang ◽  
Yunfeng Chen ◽  
Xiaolin Liu ◽  
Shangchun Guo ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Repair ◽  
Composite Hydrogel ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Good Biocompatibility

A novel in situ formed composite hydrogel based on the phototriggered imine crosslink mechanism with good biocompatibility and osteoinduction is developed for bone repair.

scholarly journals Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

2021 ◽  
Vol Volume 16 ◽  
pp. 6693-6718
Author(s):  
Chuan Fu ◽  
Yikun Jiang ◽  
Xiaoyu Yang ◽  
Yu Wang ◽  
Wei Ji ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Gold Nanoparticle ◽  
Bone Defect ◽  
Composite Scaffolds ◽  
Bone Defect Repair ◽  
Oxide Composite ◽  
Defect Repair

scholarly journals Mitochondria transfer enhances bone marrow mesenchymal stem cell functions and promotes bone defect healing

2020 ◽  
Author(s):  
Yusi Guo ◽  
Xiaopei Chi ◽  
Yifan Wang ◽  
Boon Chin Heng ◽  
Yan Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Bone Defect ◽  
Atp Production ◽  
Bone Defect Repair ◽  
Mitochondria Transfer ◽  
Defect Repair

Abstract Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is considered a promising therapeutic approach for bone defect repair. However, during the transplantation procedure, the functions and viability of BMSCs may be impaired due to extended durations of in vitro culture, aging and disease conditions of patients. Inspired by spontaneous intercellular mitochondria transfer that naturally occurs within injured tissues to rescue cellular or tissue function, we investigated whether artificial mitochondria transfer into pre-transplant BMSCs in vitro could improve cellular function and enhance their therapeutic effects on bone defect repair in situ. Methods: First, mitochondria were isolated from donor BMSCs and transferred into recipient BMSCs of the same passage. Afterwards, changes in proliferative capability was evaluated by Cell Counting Kit-8, Ki67 staining, etc., while Transwell, wound scratch healing and cell motility tests were conducted to determine migration ability. Then, alkaline phosphatase (ALP) staining, Alizarin Red staining, combined with qPCR and Western Blot experiments of Runx2 and BMP2 were performed to elucidate the effect of mitochondria transfer on the osteogenic potential of BMSCs in vitro. After that, the in vivo experiments were completed by transplanting mitochondria-recipient BMSCs into a rat cranial critical-size bone defect model. Micro CT scanning and histological analysis were conducted 4 weeks and 8 weeks after transplantation to evaluate the osteogenesis effect in situ. Finally, in order to discover the potential connection between cellular behavioral changes and aerobic metabolism, OXPHOS (oxidative phosphorylation) and ATP production were assessed and inhibition of aerobic respiration by oligomycin was proceeded. Results: Mitochondria-recipient BMSCs exhibited significantly enhanced proliferation and migration, and increased osteogenic differentiation upon osteogenic induction. The in vivo results showed more new bone formation after transplantation of mitochondria-recipient BMSCs in situ. Increased OXPHOS activity and ATP production were further observed, whereas the inhibition of which impaired the enhancement of proliferation, migration and osteogenic differentiation induced by mitochondria transfer. Conclusions: Mitochondria transfer is a feasible technique to enhance BMSCs function in vitro and promote bone defect repair in situ through the up-regulation of aerobic metabolism. The results indicated that mitochondria transfer may be a novel promising technique for optimizing stem cell function.

scholarly journals Mitochondria transfer enhances proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cell and promotes bone defect healing

2020 ◽  
Author(s):  
Yusi Guo ◽  
Xiaopei Chi ◽  
Yifan Wang ◽  
Boon Chin Heng ◽  
Yan Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Bone Defect ◽  
Atp Production ◽  
Bone Defect Repair ◽  
Mitochondria Transfer ◽  
Defect Repair

Abstract Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is considered a promising therapeutic approach for bone defect repair. However, during the transplantation procedure, the functions and viability of BMSCs may be impaired due to extended durations of in vitro culture, aging and disease conditions of patients. Inspired by spontaneous intercellular mitochondria transfer that naturally occurs within injured tissues to rescue cellular or tissue function, we investigated whether artificial mitochondria transfer into pre-transplant BMSCs in vitro could improve cellular function and enhance their therapeutic effects on bone defect repair in situ . Methods: Mitochondria were isolated from donor BMSCs and transferred into recipient BMSCs of the same batch and passage. Subsequently, changes in proliferative capacity and cell senescence were evaluated by live cell imaging, Cell Counting Kit-8 assay, cell cycle analysis, Ki67 staining, qPCR and Western Blot analysis of C-myc expression, and β-galactosidase staining. Migration ability was evaluated by the transwell migration assay, wound scratch healing and cell motility tests. Alakine phosphatase (ALP) staining, Alizarin Red staining, combined qPCR and Western Blot analyses of Runx2 and BMP2 were performed to elucidate the effects of mitochondria transfer on the osteogenic potential of BMSCs in vitro . After that, in vivo experiments were performed by transplanting mitochondria-recipient BMSCs into a rat cranial critical-size bone defect model. Micro CT scanning and histological analysis were conducted at 4 and 8 weeks after transplantation to evaluate osteogenesis in situ . Finally, in order to establish the correlation between cellular behavioral changes and aerobic metabolism, OXPHOS (oxidative phosphorylation) and ATP production were assessed and inhibition of aerobic respiration by oligomycin was performed. Results: Mitochondria-recipient BMSCs exhibited significantly enhanced proliferation and migration, and increased osteogenesis upon osteogenic induction. The in vivo results showed more new bone formation after transplantation of mitochondria-recipient BMSCs in situ . Increased OXPHOS activity and ATP production were observed, which upon inhibition by oligomycin attenuated the enhancement of proliferation, migration and osteogenic differentiation induced by mitochondria transfer. Conclusions: Mitochondria transfer is a feasible technique to enhance BMSCs function in vitro and promote bone defect repair in situ through the up-regulation of aerobic metabolism. The results indicated that mitochondria transfer may be a novel promising technique for optimizing stem cell therapeutic function.

scholarly journals Bone defect repair on the alveolar wall of the maxillary sinus using collagen membranes and temporal fascia: an experimental study in monkeys

2011 ◽  
Vol 77 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Adalberto Novaes Silva ◽  
José Américo de Oliveira ◽  
Maria Célia Jamur ◽  
José Ari Gualberto Junqueira ◽  
Vani Maria Correa ◽  
...  
Keyword(s):  
Experimental Study ◽  
Maxillary Sinus ◽  
Bone Defect ◽  
Alveolar Wall ◽  
Bone Defect Repair ◽  
Temporal Fascia ◽  
Defect Repair ◽  
Collagen Membranes

Clay-based nanocomposite hydrogel with attractive mechanical properties and sustained bioactive ion release for bone defect repair

2021 ◽  
Author(s):  
Xinyun Zhai ◽  
Changshun Ruan ◽  
Jie Shen ◽  
Chuping Zheng ◽  
Xiaoli Zhao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Defect ◽  
Nanocomposite Hydrogel ◽  
Ion Release ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Gradual Release

Using nanoclay as the physical crosslinker, a novel clay-based nanocomposite hydrogel with attractive mechanical properties has be obtained, and the gradual release of intrinsic Mg2+ and Si4+ endows the system with excellent osteogenesis.

Injectable gel graft for bone defect repair

2014 ◽  
Vol 9 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Josephine Fang ◽  
Zhi Yang ◽  
ShihJye Tan ◽  
Charisse Tayag ◽  
Marcel E Nimni ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Defect Repair ◽  
Defect Repair

Nano-Hydroxyapatite Scaffold Based on Recombinant Human Bone Morphogenetic Protein 2 and Its Application in Bone Defect Repair

2021 ◽  
Vol 17 (7) ◽  
pp. 1330-1338
Author(s):  
Shibai Zhu ◽  
Xiaotian Zhang ◽  
Xi Chen ◽  
Yiou Wang ◽  
Shanni Li ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Artificial Bone ◽  
Bone Defect Repair ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Defect Repair ◽  
Hydroxyapatite Scaffold ◽  
Recombinant Human Bone

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

Local Elimination of Senescent Cells Promotes Bone Defect Repair during Aging

2022 ◽  
Author(s):  
Xiaotao Xing ◽  
Haisen Huang ◽  
Xin Gao ◽  
Jian Yang ◽  
Qi Tang ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Defect Repair ◽  
Defect Repair
Sign in / Sign up

Export Citation Format

Share Document