scholarly journals Biomimetic Photocurable Three-Dimensional Printed Nerve Guidance Channels with Aligned Cryomatrix Lumen for Peripheral Nerve Regeneration

2018 ◽  
Vol 10 (50) ◽  
pp. 43327-43342 ◽  
Author(s):  
Anamika Singh ◽  
Sanja Asikainen ◽  
Arun K. Teotia ◽  
Parvaiz A. Shiekh ◽  
Eero Huotilainen ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Three Dimensional ◽  
Guidance Channels

Producing Neurospheroids and Hydrogels to Create a Three-dimensional in Vitro Model for the Use of Conduits in Peripheral Nerve Regeneration

Neurosurgery ◽  
2019 ◽  
Vol 84 (5) ◽  
pp. E272-E272
Author(s):  
Devyani Shete ◽  
Aran Batth ◽  
Aditi Nijhawan ◽  
Jaffer Choudhary ◽  
Ian Thompson
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
3D Structure ◽  
Three Dimensional ◽  
Negative Control ◽  
Cellular Growth ◽  
Live Cells

Abstract INTRODUCTION Peripheral nerve regeneration is a complex challenge that requires suitable nerve guidance systems to bridge the severed ends of 2 nerves back together. Current polymeric conduits on the market provide good cellular growth but are limited by the length of gap defect they can repair, and complete functional recovery is rare. This project focused on creating a three-dimensional (3D) in Vitro spheroidal sprouting assay for peripheral nerve regeneration, as well as producing and testing different polymeric hydrogels as potential scaffold materials for the conduit. METHODS Different concentrations of chitosan, methylcellulose (MC) and sodium alginate were produced, as well as blends of these materials. These hydrogels were seeded with 3D neurospheroids, along with NG108-15 (neuronal) cells and Schwann cells to test their biocompatibility. RESULTS MTT assays showed the mean absorbance of chitosan gels with NG108-15 cells at 24 hr (P < .001) and 72 hr (P > .05) was similar/slightly higher than the negative control. Live-Dead data showed 93.4% of live cells at DIV7 on MC: Ch blends, compared to 72% with chitosan alone. CONCLUSION Overall, both chitosan and MC were nontoxic and biocompatible with NG108-15 and Schwann cells. Blending chitosan with MC improved its chemical and physical properties. The cells formed spheroids that well on a gel; this pseudo-3D structure is excellent for research purposes compared to 2D as it mimics the body's internal environment.

Blind-ended semipermeable guidance channels support peripheral nerve regeneration in the absence of a distal nerve stump

1988 ◽  
Vol 454 (1-2) ◽  
pp. 179-187 ◽  
Author(s):  
P. Aebischer ◽  
V. Gue´nard ◽  
S.R. Winn ◽  
R.F. Valentini ◽  
P.M. Galletti
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Stump ◽  
Distal Nerve ◽  
Guidance Channels

Polymer electret guidance channels enhance peripheral nerve regeneration in mice

1989 ◽  
Vol 480 (1-2) ◽  
pp. 300-304 ◽  
Author(s):  
R.F. Valentini ◽  
A.M. Sabatini ◽  
P. Dario ◽  
P. Aebischer
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Guidance Channels

scholarly journals 3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

2020 ◽  
Vol 8 ◽  
Author(s):  
Yu-Jie Lin ◽  
Yun-Wei Lee ◽  
Che-Wei Chang ◽  
Chieh-Cheng Huang
Keyword(s):  
Peripheral Nerve ◽  
Cord Blood ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Therapeutic Potential ◽  
Peripheral Nerve Regeneration ◽  
Three Dimensional ◽  
3D Spheroids

Schwann cells (SCs) are promising candidates for cell therapy due to their ability to promote peripheral nerve regeneration. However, SC-based therapies are hindered by the lack of a clinically renewable source of SCs. In this study, using a well-defined non-genetic approach, umbilical cord blood mesenchymal stem cells (cbMSCs), a clinically applicable cell type, were phenotypically, epigenetically, and functionally converted into SC-like cells (SCLCs) that stimulated effective sprouting of neuritic processes from neuronal cells. To further enhance their therapeutic capability, the cbMSC-derived SCLCs were assembled into three-dimensional (3D) cell spheroids by using a methylcellulose hydrogel system. The cell–cell and cell–extracellular matrix interactions were well-preserved within the formed 3D SCLC spheroids, and marked increases in neurotrophic, proangiogenic and anti-apoptotic factors were detected compared with cells that were harvested using conventional trypsin-based methods, demonstrating the superior advantage of SCLCs assembled into 3D spheroids. Transplantation of 3D SCLC spheroids into crush-injured rat sciatic nerves effectively promoted the recovery of motor function and enhanced nerve structure regeneration. In summary, by simply assembling cells into a 3D-spheroid conformation, the therapeutic potential of SCLCs derived from clinically available cbMSCs for promoting nerve regeneration was enhanced significantly. Thus, these cells hold great potential for translation to clinical applications for treating peripheral nerve injury.

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