Tellurium/Bovine Serum Albumin Nanocomposites Inducing the Formation of Stress Granules in a Protein Kinase R-Dependent Manner

2018 ◽  
Vol 10 (30) ◽  
pp. 25241-25251 ◽  
Author(s):  
Yanrong Zhou ◽  
Yanli Bai ◽  
Huabing Liu ◽  
Xiaohan Jiang ◽  
Ting Tong ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Stress Granules ◽  
Dependent Manner ◽  
Protein Kinase R

Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation

Development ◽  
1995 ◽  
Vol 121 (4) ◽  
pp. 1129-1137 ◽  
Author(s):  
P.E. Visconti ◽  
J.L. Bailey ◽  
G.D. Moore ◽  
D. Pan ◽  
P. Olds-Clarke ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Tyrosine Phosphorylation ◽  
Bovine Serum ◽  
Female Reproductive Tract ◽  
Dependent Manner ◽  
Protein Tyrosine Phosphorylation ◽  
Sperm Capacitation ◽  
Protein Tyrosine

The molecular basis of mammalian sperm capacitation, defined functionally as those processes that confer on the sperm the acquisition of fertilization-competence either in vivo in the female reproductive tract or in vitro, is poorly understood. We demonstrate here that capacitation of caudal epididymal mouse sperm in vitro is accompanied by a time-dependent increase in the protein tyrosine phosphorylation of a subset of proteins of M(r) 40,000-120,000. Incubation of sperm in media devoid of bovine serum albumin, CaCl2 or NaHCO3, components which individually are required for capacitation, prevent the sperm from undergoing capacitation as assessed by the ability of the cells to acquire the pattern B chlortetracycline fluorescence, to undergo the zona pellucida-induced acrosome reaction and, in some cases, to fertilize metaphase II-arrested eggs in vitro. In each of these cases the protein tyrosine phosphorylation of the subset of capacitation-associated proteins does not occur. Protein tyrosine phosphorylation of these particular proteins, as well as sperm capacitation, can be recovered in media devoid of each of these three constituents (bovine serum albumin, CaCl2 or NaHCO3) by adding back the appropriate component in a concentration-dependent manner. The requirement of NaHCO3 for these phosphorylations is not due to an alkalinization of intracellular sperm pH or to an increase in media pH. Caput epididymal sperm, which lack the ability to undergo capacitation in vitro, do not display this capacitation-dependent subset of tyrosine phosphorylated proteins in complete media even after extended incubation periods, and do not fertilize metaphase II-arrested eggs in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Bovine serum albumin as a resuscitation promoting factor for viable but non-culturable Mycobacterium tuberculosis via the activation of protein kinase A-dependent cellular processes

2021 ◽  
Author(s):  
Yuta Morishige ◽  
Yoshiro Murase ◽  
Kinuyo Chikamatsu ◽  
Akio Aono ◽  
Yuriko Igarashi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Protein Kinase ◽  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Kinase A ◽  
Bovine Serum ◽  
Vbnc State ◽  
Kinase A

Objective: Mycobacterium tuberculosis (Mtb) H37Ra strain has been reported to rapidly enter the viable but non-culturable (VBNC) state following treatment with an NADH oxidase inhibitor (diphenyleneiodonium [DPI]) and to be resuscitated by fetal bovine serum (FBS). However, the mechanism underlying FBS-induced resuscitation is currently unclear. We tried to reveal the underlying mechanism of FBS-induced resuscitation using M. tuberculosis H37Rv. Methods: First, we evaluated the effect of DPI on culturability, viability and changes of cellular phenotypes toward H37Rv. Secondly, we measured the resuscitation-promoting effects of human serum albumin, egg-white albumin, N-acetyl-L-cysteine, and D-mannitol in DPI-induced VBNC cells, as antioxidative agents have been reported to be key molecules for resuscitation of other microbes. We also evaluated the effect of inhibition of cAMP production and protein kinase A on BSA-induced resuscitation. Results: DPI treatment successfully induced a VBNC state in H37Rv, resulting in a low proportion of culturable cells, loss of acid-fastness and lipid-accumulation but a high proportion of viable cells. Not only FBS but also bovine serum albumin (BSA) alone could resuscitate H37Rv. Contrary to our expectation, only human serum albumin had a similar resuscitative effect to BSA. The inhibition of adenylyl cyclase by SQ22536 did not have a significant effect on resuscitation; however, the inhibition of protein kinase A by H89 strongly suppressed the BSA-induced resuscitation. Conclusion: DPI-induced VBNC Mtb cells may be resuscitated via the activation of protein kinase A-dependent processes through interaction with BSA.

scholarly journals β-glucan: Immune boosting potential and antioxidant candidate

2020 ◽  
Vol 11 (1) ◽  
pp. 491-496
Author(s):  
Abhishek Kamboj ◽  
Aanchal Jain ◽  
Tanya Singh ◽  
Ajam Shaikh ◽  
Amit Gupta
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Specific Protein ◽  
Antioxidant Potential ◽  
Cell Culture Supernatant ◽  
Dependent Manner ◽  
Dpph Assay ◽  
Concentration Dependent Manner ◽  
Ifn Gamma

In literature, β-glucan is abundantly found in oats and currently used as well as tested against various varieties of food products. The objective of our study is to evaluate its immune-boosting potential of β-glucan extracted from oats and tested against specific protein antigen i.e., bovine serum albumin (BSA) and also measure its antioxidant activity. For these studies, β-glucan using variable concentration and tested in human whole blood samples (exposed with an optimized concentration of bovine serum albumin, BSA) and examined its immune-boosting potential. In contrast, estimation of Th1 (IFN-gamma) and Th2 (IL-4) cytokines were also measured from cell culture supernatant and also measured its antioxidant potential through DPPH assay. The results showed that β-glucan enhanced immune-boosting activity in a concentration-dependent manner against BSA. In contrast, there is enhancement in Th2 (IL-4) cytokines in a dose-dependent manner, but there is no significant effect in Th1 (IFN-gamma) as compared to control. In addition, β-glucan showed drastic enhanced antioxidant potential, which is confirmed through DPPH assay. The data demonstrate that β-glucan showing immune-boosting properties and antioxidant potential.

scholarly journals Evaluation of in vitro anti-inflammatory activity of the spadix of Colocasia affinis

2019 ◽  
Vol 19 (2) ◽  
pp. 30-34
Author(s):  
Vanlalhruaii ◽  
C. Malsawmtluangi ◽  
H. Lalhlenmawia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Protease Inhibition ◽  
Egg Albumin ◽  
Anti Inflammatory

The methanolic extract of C. affinis was evaluated for its in-vitro anti-inflammatory activity by bovine serum albumin denaturation method, egg albumin denaturation method and protease inhibition method at different concentrations. Diclofenac sodium was used as the reference drug. The extract exhibits anti-inflammatory activity in a concentration-dependent manner. In bovine serum albumin denaturation method, the extract at concentrations of 100, 200, 400, 600, 800, 1000 µg/ml showed 25.49, 28.43, 31.37, 37.25, 41.17, 48.03% inhibition respectively. In egg albumin denaturation method, the concentrations of 50, 100, 200, 300, 400, 500 µg/ml showed 5.3, 9.89, 28.12, 32.8, 43.2, 52.8% inhibition respectively. In protease inhibition method the extract at concentration of 100, 200, 300, 400, 500 µg/ml showed 8.87, 19.32, 28.56, 43.96, 59.92% inhibition respectively. Therefore, from the results it can be concluded that the methanolic extract of C. affinis possesses anti-inflammatory activity.

Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway

Development ◽  
1995 ◽  
Vol 121 (4) ◽  
pp. 1139-1150 ◽  
Author(s):  
P.E. Visconti ◽  
G.D. Moore ◽  
J.L. Bailey ◽  
P. Leclerc ◽  
S.A. Connors ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Tyrosine Phosphorylation ◽  
Bovine Serum ◽  
Biologically Active ◽  
Dependent Manner ◽  
Protein Tyrosine Phosphorylation ◽  
Concentration Dependent Manner ◽  
Mouse Sperm ◽  
Protein Tyrosine

In the accompanying report (Visconti, P.E., Bailey, J.L., Moore, G.D., Pan, D., Olds-Clarke, P. and Kopf, G.S. (1995) Development, 121, 1129–1137) we demonstrated that the tyrosine phosphorylation of a subset of mouse sperm proteins of M(r) 40,000-120,000 was correlated with the capacitation state of the sperm. The mechanism by which protein tyrosine phosphorylation is regulated in sperm during this process is the subject of this report. Cauda epididymal sperm, when incubated in media devoid of NaHCO3, CaCl2 or bovine serum albumin do not display the capacitation-associated increases in protein tyrosine phosphorylation of this subset of proteins. This NaHCO3, CaCl2 or bovine serum albumin requirement for protein tyrosine phosphorylation can be completely overcome by the addition of biologically active, but not inactive, cAMP analogues. Addition of the active cAMP analogues to sperm incubated in media devoid of NaHCO3, CaCl2 or bovine serum albumin overcomes the inability of these media to support capacitation, as assessed by the ability of the cells to acquire the pattern B chlortetracycline fluorescence, to undergo the zona pellucida-induced acrosome reaction and, in some cases, to fertilize metaphase II-arrested eggs in vitro. The effects of the cAMP analogues to enhance protein tyrosine phosphorylation and to promote capacitation appears to be at the level of the cAMP-dependent protein kinase (PKA), since two specific inhibitors of this enzyme (H-89 and Rp-cAMPS) block the capacitation-dependent increases in protein tyrosine phosphorylation in sperm incubated in media supporting capacitation. Capacitation, as assessed by the aforementioned endpoints, also appears to be inhibited by H-89 in a concentration-dependent manner. These results provide further evidence for the interrelationship between protein tyrosine phosphorylation and the appearance of the capacitated state in mouse sperm. They also demonstrate that both protein tyrosine phosphorylation and capacitation appear to be regulated by cAMP/PKA. Up-regulation of protein tyrosine phosphorylation by cAMP/PKA in sperm is, to our knowledge, the first demonstration of such an interrelationship between tyrosine kinase/phosphatase and PKA signaling pathways.

Bovine Serum Albumin Enhances Silver Nanoparticle Dissolution Kinetics in a Size- and Concentration-Dependent Manner

Langmuir ◽  
2020 ◽  
Vol 36 (4) ◽  
pp. 1053-1061 ◽  
Author(s):  
Daniel J. Boehmler ◽  
Zachary J. O’Dell ◽  
Christopher Chung ◽  
Kathryn R. Riley
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silver Nanoparticle ◽  
Bovine Serum ◽  
Dissolution Kinetics ◽  
Dependent Manner ◽  
Concentration Dependent Manner

scholarly journals Gliding Motility of Mycoplasma mobile Can Occur by Repeated Binding to N-Acetylneuraminyllactose (Sialyllactose) Fixed on Solid Surfaces

2006 ◽  
Vol 188 (18) ◽  
pp. 6469-6475 ◽  
Author(s):  
Ryoichiro Nagai ◽  
Makoto Miyata
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gliding Motility ◽  
Solid Surfaces ◽  
Dependent Manner ◽  
Animal Cells ◽  
Unknown Mechanism ◽  
Direct Binding ◽  
Lines Of Evidence

ABSTRACT Mycoplasma mobile relies on an unknown mechanism to glide across solid surfaces including glass, animal cells, and plastics. To identify the direct binding target, we examined the factors that affect the binding of Mycoplasma pneumoniae to solid surfaces and concluded that N-acetylneuraminyllactose (sialyllactose) attached to a protein can mediate glass binding on the basis of the following four lines of evidence: (i) glass binding was inhibited by N-acetylneuraminidase, (ii) glass binding was inhibited by N-acetylneuraminyllactose in a structure-dependent manner, (iii) binding occurred on glass pretreated with bovine serum albumin attached to N-acetylneuraminyllactose, and (iv) gliding speed depended on the density of N-acetylneuraminyllactose on glass.

Protein kinase C activity during the process of lung liquid clearance

1993 ◽  
Vol 265 (1) ◽  
pp. L57-L66 ◽  
Author(s):  
M. Sapijaszko ◽  
J. Mackenzie ◽  
M. P. Walsh ◽  
Y. Berthiaume
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inflammatory Cells ◽  
Kinase C ◽  
Protein Kinase C Activity ◽  
Heterologous Serum ◽  
Lung Liquid

Although active transport of sodium plays an important role in the resolution of pulmonary edema, the biochemical regulation of this process is still under investigation. The purpose of this study was to evaluate the activity of protein kinase C during the process of lung liquid clearance. Alveolar flooding was induced by instilling 5% bovine serum albumin solution, saline, or heterologous serum in the air spaces of rats. The activity of protein kinase C was measured in both the instilled and control lungs at 10 min and 1 and 4 h after fluid instillation. Four hours after instillation of 5% bovine serum albumin, the ratio of protein kinase C activity in the instilled lung compared with the control lung was 2.2 +/- 0.3. Similar results were obtained following instillation with heterologous serum or saline. Since we measured a clearance rate of 0.8 ml/h in anesthetized rats, we can postulate that the activation of protein kinase C occurred when > 40% of the liquid had been cleared from the lung. This increased activity of protein kinase C was not due to an increase in kinase activity in the inflammatory cells or an increase in enzyme quantity but due to a decrease of protein kinase C inhibitory activity in the lung. These results suggest that protein kinase C second messenger system may play a regulatory role in lung liquid clearance.

Sign in / Sign up

Export Citation Format

Share Document