Ultrasensitive Simultaneous Detection of Multiplex Disease-Related Nucleic Acids Using Double-Enhanced Surface-Enhanced Raman Scattering Nanosensors

2018 ◽  
Vol 10 (30) ◽  
pp. 25770-25778 ◽  
Author(s):  
Ruiyan Guo ◽  
Fangfei Yin ◽  
Yudie Sun ◽  
Lan Mi ◽  
Lin Shi ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Nucleic Acids ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Enhanced Surface

scholarly journals Duplex Surface Enhanced Raman Scattering-Based Lateral Flow Immunosensor for the Low-Level Detection of Antibiotic Residues in Milk

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5249
Author(s):  
Ruiqi Fan ◽  
Shusheng Tang ◽  
Sunlin Luo ◽  
Hu Liu ◽  
Wanjun Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Lateral Flow ◽  
Antibiotic Residues ◽  
Relative Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Analytical Approaches

A duplex surface enhanced Raman scattering (SERS)-based lateral flow immunosensor was established for the simultaneous detection of two common antibiotic residues including tetracycline and penicillin in milk. The newly synthesized Au@Ag nanoparticles were labeled with different Raman molecules including 5,5-dithiobis-2-nitrobenzoic acid (DTNB) or 4-mercaptobenzoic acid (MBA), followed by the conjugation of anti-tetracycline monoclonal antibody or anti-penicillin receptor, forming two kinds of SERS nanoprobes. The two nanoprobes can recognize tetracycline-BSA and ampicillin-BSA, respectively, which facilitates the simultaneous detection of the two types of antibiotics on a single test line. After optimization, detection limits of tetracycline and penicillin as low as 0.015 ng/mL and 0.010 ng/mL, respectively, were achieved. These values were far below those of most of other documented bio-analytical approaches. Moreover, the spiking test demonstrates an excellent assay accuracy with recoveries of 88.8% to 111.3%, and satisfactory assay precision with relative standard deviation below 16%. Consequently, the results demonstrate that the SERS-based lateral flow immunosensor developed in this study has the advantages of excellent assay sensitivity and remarkable multiplexing capability, thus it will have great application potential in food safety monitoring.

Enhanced surface-enhanced Raman scattering performance by folding silver nanorods

2012 ◽  
Vol 100 (11) ◽  
pp. 113101 ◽  
Author(s):  
Qin Zhou ◽  
Xian Zhang ◽  
Yu Huang ◽  
Zhengcao Li ◽  
Yiping Zhao ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Silver Nanorods ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Enhanced Surface
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