Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair

2018 ◽  
Vol 10 (21) ◽  
pp. 17742-17755 ◽  
Author(s):  
Lei Fan ◽  
Can Liu ◽  
Xiuxing Chen ◽  
Yan Zou ◽  
Zhengnan Zhou ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cell ◽  
Cell Fate ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Induced Pluripotent Stem Cell ◽  
Stem Cell Fate ◽  
Injury Repair ◽  
Cord Injury ◽  
Induced Pluripotent

scholarly journals Beneficial Effect of Human Induced Pluripotent Stem Cell-Derived Neural Precursors in Spinal Cord Injury Repair

2015 ◽  
Vol 24 (9) ◽  
pp. 1781-1797 ◽  
Author(s):  
Nataliya Romanyuk ◽  
Takashi Amemori ◽  
Karolina Turnovcova ◽  
Pavel Prochazka ◽  
Brigitte Onteniente ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Beneficial Effect ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Neural Precursors ◽  
Injury Repair ◽  
Cord Injury ◽  
Induced Pluripotent

Increase of sensitivity to mechanical stimulus after transplantation of murine induced pluripotent stem cell–derived astrocytes in a rat spinal cord injury model

2011 ◽  
Vol 15 (6) ◽  
pp. 582-593 ◽  
Author(s):  
Koichi Hayashi ◽  
Masayuki Hashimoto ◽  
Masao Koda ◽  
Atsuhiko T. Naito ◽  
Atsushi Murata ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Mechanical Stimulus ◽  
Cord Injury ◽  
Induced Pluripotent

Object Clinical use of autologous induced pluripotent stem cells (iPSCs) could circumvent immune rejection and bioethical issues associated with embryonic stem cells. Spinal cord injury (SCI) is a devastating trauma with long-lasting disability, and current therapeutic approaches are not satisfactory. In the present study, the authors used the neural stem sphere (NSS) method to differentiate iPSCs into astrocytes, which were evaluated after their transplantation into injured rat spinal cords. Methods Induced pluripotent stem cell–derived astrocytes were differentiated using the NSS method and injected 3 and 7 days after spinal contusion–based SCI. Control rats were injected with DMEM in the same manner. Locomotor recovery was assessed for 8 weeks, and sensory and locomotion tests were evaluated at 8 weeks. Immunohistological parameters were then assessed. Results Transplant recipients lived for 8 weeks without tumor formation. Transplanted cells stretched their processes along the longitudinal axis, but they did not merge with the processes of host GFAP-positive astrocytes. Locomotion was assessed in 3 ways, but none of the tests detected statistically significant improvements compared with DMEM-treated control rats after 8 weeks. Rather, iPSC transplantation caused even greater sensitivity to mechanical stimulus than DMEM treatment. Conclusions Astrocytes can be generated by serum treatment of NSS-generated cells derived from iPSCs. However, transplantation of such cells is poorly suited for repairing SCI.

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