Cell-Shaping Micropatterns for Quantitative Super-Resolution Microscopy Imaging of Membrane Mechanosensing Proteins

2017 ◽  
Vol 9 (33) ◽  
pp. 27575-27586 ◽  
Author(s):  
Anthony Fernandez ◽  
Markville Bautista ◽  
Ramunas Stanciauskas ◽  
Taerin Chung ◽  
Fabien Pinaud
Keyword(s):  
Super Resolution ◽  
Microscopy Imaging ◽  
Cell Shaping ◽  
Super Resolution Microscopy

scholarly journals Design of experiments for the optimization of SOFI super-resolution microscopy imaging

2021 ◽  
Vol 12 (5) ◽  
pp. 2617
Author(s):  
Dario Cevoli ◽  
Raffaele Vitale ◽  
Wim Vandenberg ◽  
Siewert Hugelier ◽  
Robin Van den Eynde ◽  
...  
Keyword(s):  
Design Of Experiments ◽  
Super Resolution ◽  
Microscopy Imaging ◽  
Super Resolution Microscopy

scholarly journals A Quantitative Platform for Super-Resolution Microscopy Imaging

2017 ◽  
Vol 112 (3) ◽  
pp. 143a
Author(s):  
Ottavia Golfetto ◽  
Devin L. Wakefield ◽  
Eliedonna E. Cacao ◽  
Kendra N. Avery ◽  
Raphael Jorand ◽  
...  
Keyword(s):  
Super Resolution ◽  
Microscopy Imaging ◽  
Super Resolution Microscopy

scholarly journals Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?

2021 ◽  
Vol 22 (13) ◽  
pp. 6730
Author(s):  
Paweł Matryba ◽  
Kacper Łukasiewicz ◽  
Monika Pawłowska ◽  
Jacek Tomczuk ◽  
Jakub Gołąb
Keyword(s):  
Large Scale ◽  
Rapid Development ◽  
Super Resolution ◽  
Molecular Probes ◽  
Dimensional Structure ◽  
Fluorescence Signal ◽  
Microscopy Imaging ◽  
Optical Clearing ◽  
Super Resolution Microscopy ◽  
Tissue Optical Clearing

The rapid development of super-resolution microscopy (SRM) techniques opens new avenues to examine cell and tissue details at a nanometer scale. Due to compatibility with specific labelling approaches, in vivo imaging and the relative ease of sample preparation, SRM appears to be a valuable alternative to laborious electron microscopy techniques. SRM, however, is not free from drawbacks, with the rapid quenching of the fluorescence signal, sensitivity to spherical aberrations and light scattering that typically limits imaging depth up to few micrometers being the most pronounced ones. Recently presented and robustly optimized sets of tissue optical clearing (TOC) techniques turn biological specimens transparent, which greatly increases the tissue thickness that is available for imaging without loss of resolution. Hence, SRM and TOC are naturally synergistic techniques, and a proper combination of these might promptly reveal the three-dimensional structure of entire organs with nanometer resolution. As such, an effort to introduce large-scale volumetric SRM has already started; in this review, we discuss TOC approaches that might be favorable during the preparation of SRM samples. Thus, special emphasis is put on TOC methods that enhance the preservation of fluorescence intensity, offer the homogenous distribution of molecular probes, and vastly decrease spherical aberrations. Finally, we review examples of studies in which both SRM and TOC were successfully applied to study biological systems.

scholarly journals Structure illumination microscopy imaging of lipid vesicles in live bacteria with naphthalimide-appended organometallic complexes

2021 ◽  
Author(s):  
Anna Maria Ranieri ◽  
Matteo Vezzelli ◽  
Kathryn Leslie ◽  
Song Huang ◽  
Stefano Stagni ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Super Resolution ◽  
Lipid Vesicles ◽  
Molecular Probes ◽  
Organometallic Complexes ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Microscopy Imaging ◽  
Multiple Imaging ◽  
Super Resolution Microscopy

There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(I) or platinum(II) in the current study, enables techniques such as nanoSIMS. While the rhenium(I) complex was not sufficiently stable to be used as probe, the platinum(II) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live <i>Bacillus cereus</i> confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(II) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria.<br>

Nanometer resolved single-molecule colocalization of nuclear factors by two-color super resolution microscopy imaging

Methods ◽  
2016 ◽  
Vol 105 ◽  
pp. 44-55 ◽  
Author(s):  
Mariya Georgieva ◽  
Diego I. Cattoni ◽  
Jean-Bernard Fiche ◽  
Thibaut Mutin ◽  
Delphine Chamousset ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Microscopy Imaging ◽  
Nuclear Factors ◽  
Super Resolution Microscopy

scholarly journals Bioothogonally applicable, π-extended rhodamines for super-resolution microscopy imaging for intracellular proteins

2020 ◽  
Vol 28 (1) ◽  
pp. 115218
Author(s):  
Alexandra Egyed ◽  
Attila Kormos ◽  
Bianka Söveges ◽  
Krisztina Németh ◽  
Péter Kele
Keyword(s):  
Super Resolution ◽  
Microscopy Imaging ◽  
Intracellular Proteins ◽  
Super Resolution Microscopy

Research on the Super-Resolution Microscopy Imaging Based on Random Pulse Coding

2016 ◽  
Vol 53 (7) ◽  
pp. 071101
Author(s):  
谢创亮 Xie Chuangliang ◽  
观嘉宁 Guan Jianing ◽  
白玉磊 Bai Yulei ◽  
杨其宇 Yang Qiyu ◽  
叶双莉 Ye Shuangli ◽  
...  
Keyword(s):  
Super Resolution ◽  
Microscopy Imaging ◽  
Random Pulse ◽  
Super Resolution Microscopy
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