Antibody Conjugated, Raman Tagged Hollow Gold–Silver Nanospheres for Specific Targeting and Multimodal Dark-Field/SERS/Two Photon-FLIM Imaging of CD19(+) B Lymphoblasts

2017 ◽  
Vol 9 (25) ◽  
pp. 21155-21168 ◽  
Author(s):  
Timea Nagy-Simon ◽  
Andra-Sorina Tatar ◽  
Ana-Maria Craciun ◽  
Adriana Vulpoi ◽  
Maria-Ancuta Jurj ◽  
...  
Keyword(s):  
Dark Field ◽  
Two Photon ◽  
Specific Targeting ◽  
Gold Silver ◽  
Silver Nanospheres

Hyperspectral Dark Field Optical Microscopy of Single Silver Nanospheres

2016 ◽  
Vol 120 (13) ◽  
pp. 7295-7298 ◽  
Author(s):  
Patrick Z. El-Khoury ◽  
Alan G. Joly ◽  
Wayne P. Hess
Keyword(s):  
Optical Microscopy ◽  
Dark Field ◽  
Silver Nanospheres

Near-infrared Emitting Gold-silver Nanoclusters with Large Stokes Shift for Two-Photon in Vivo Imaging

2021 ◽  
Author(s):  
Yaowei Peng ◽  
Xiaoyu Huang ◽  
Fu Wang
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Silver Nanoclusters ◽  
One Pot ◽  
Two Photon ◽  
Nir Fluorescence ◽  
Gold Silver ◽  
Stokes Shifts

Near-infrared emitting bi-metallic gold/silver nanoclusters with great stokes shifts were manufactured through one-pot synthesis. The gold/silver nanoclusters exhibit strong NIR fluorescence due to the silver effect, which can be applied...

Design and synthesis of fluorenone-based dyes: two-photon excited fluorescent probes for imaging of lysosomes and mitochondria in living cells

2015 ◽  
Vol 3 (16) ◽  
pp. 3315-3323 ◽  
Author(s):  
A. L. Capodilupo ◽  
V. Vergaro ◽  
E. Fabiano ◽  
Milena De Giorgi ◽  
F. Baldassarre ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Design And Synthesis ◽  
Two Photon ◽  
Specific Targeting

Three fluorenone-based two-photon fluorescent probes for specific targeting of lysosomes and mitochondria in cancer cells.

scholarly journals Advances in the Development of Phage-Based Probes for Detection of Bio-Species

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 30
Author(s):  
Kameshpandian Paramasivam ◽  
Yuanzhao Shen ◽  
Jiasheng Yuan ◽  
Ibtesam Waheed ◽  
Chuanbin Mao ◽  
...  
Keyword(s):  
Metal Nanoparticles ◽  
Plasmon Resonance ◽  
Resonance Effect ◽  
Dark Field ◽  
Biological Species ◽  
Detection Methods ◽  
Arbitrary Sequence ◽  
Single Chain ◽  
Animal Cells ◽  
Gold Silver

Bacteriophages, abbreviated as “phages”, have been developed as emerging nanoprobes for the detection of a wide variety of biological species, such as biomarker molecules and pathogens. Nanosized phages can display a certain length of exogenous peptides of arbitrary sequence or single-chain variable fragments (scFv) of antibodies that specifically bind to the targets of interest, such as animal cells, bacteria, viruses, and protein molecules. Metal nanoparticles generally have unique plasmon resonance effects. Metal nanoparticles such as gold, silver, and magnetism are widely used in the field of visual detection. A phage can be assembled with metal nanoparticles to form an organic–inorganic hybrid probe due to its nanometer-scale size and excellent modifiability. Due to the unique plasmon resonance effect of this composite probe, this technology can be used to visually detect objects of interest under a dark-field microscope. In summary, this review summarizes the recent advances in the development of phage-based probes for ultra-sensitive detection of various bio-species, outlining the advantages and limitations of detection technology of phage-based assays, and highlighting the commonly used editing technologies of phage genomes such as homologous recombination and clustered regularly interspaced palindromic repeats/CRISPR-associated proteins system (CRISPR-Cas). Finally, we discuss the possible scenarios for clinical application of phage-probe-based detection methods.

Utilizing Dark Field Electron Microscopy to Produce Lantern Slides

1974 ◽  
Vol 32 ◽  
pp. 116-117
Author(s):  
J. N. Meador ◽  
C. N. Sun ◽  
H. J. White
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Dark Field ◽  
Limiting Factor ◽  
Clinical Laboratories ◽  
Field Electron ◽  
Time Element ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dark Field Micrographs

The electron microscope is being utilized more and more in clinical laboratories for pathologic diagnosis. One of the major problems in the utilization of the electron microscope for diagnostic purposes is the time element involved. Recent experimentation with rapid embedding has shown that this long phase of the process can be greatly shortened. In rush cases the making of projection slides can be eliminated by taking dark field electron micrographs which show up as a positive ready for use. The major limiting factor for use of dark field micrographs is resolution. However, for conference purposes electron micrographs are usually taken at 2.500X to 8.000X. At these low magnifications the resolution obtained is quite acceptable.

Protamine-DNA interaction

1978 ◽  
Vol 36 (2) ◽  
pp. 200-201
Author(s):  
D.P. Bazett-Jones ◽  
F.P. Ottensmeyer
Keyword(s):  
Small Volume ◽  
Double Helix ◽  
Dna Interaction ◽  
Dark Field ◽  
Sperm Head ◽  
Nuclear Proteins ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dna Double Helix ◽  
Positively Charged

Dark field electron microscopy has been used for the study of the structure of individual macromolecules with a resolution to at least the 5Å level. The use of this technique has been extended to the investigation of structure of interacting molecules, particularly the interaction between DNA and fish protamine, a class of basic nuclear proteins of molecular weight 4,000 daltons.Protamine, which is synthesized during spermatogenesis, binds to chromatin, displaces the somatic histones and wraps up the DNA to fit into the small volume of the sperm head. It has been proposed that protamine, existing as an extended polypeptide, winds around the minor groove of the DNA double helix, with protamine's positively-charged arginines lining up with the negatively-charged phosphates of DNA. However, viewing protamine as an extended protein is inconsistent with the results obtained in our laboratory.

Sign in / Sign up

Export Citation Format

Share Document