Hyaluronic Acid-Coated Silver Nanoparticles As a Nanoplatform for in Vivo Imaging Applications

2016 ◽  
Vol 8 (39) ◽  
pp. 25650-25653 ◽  
Author(s):  
Xin Zhang ◽  
Meinan Yao ◽  
Muhua Chen ◽  
Liqiang Li ◽  
Chengyan Dong ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
In Vivo Imaging

Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

2020 ◽  
Vol 238 ◽  
pp. 116175 ◽  
Author(s):  
M.R. El-Aassar ◽  
Omar M. Ibrahim ◽  
Moustafa M.G. Fouda ◽  
Nagham G. El-Beheri ◽  
Mona M. Agwa
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
In Vivo Studies

scholarly journals In Vivo Imaging of Transport and Biocompatibility of Single Silver Nanoparticles in Early Development of Zebrafish Embryos

ACS Nano ◽  
2007 ◽  
Vol 1 (2) ◽  
pp. 133-143 ◽  
Author(s):  
Kerry J. Lee ◽  
Prakash D. Nallathamby ◽  
Lauren M. Browning ◽  
Christopher J. Osgood ◽  
Xiao-Hong Nancy Xu
Keyword(s):  
Silver Nanoparticles ◽  
Early Development ◽  
In Vivo Imaging ◽  
Zebrafish Embryos

scholarly journals A simple method for the synthesis of hyaluronic acid coated magnetic nanoparticles for highly efficient cell labelling and in vivo imaging

RSC Advances ◽  
2011 ◽  
Vol 1 (8) ◽  
pp. 1449 ◽  
Author(s):  
Mohammad H. El-Dakdouki ◽  
Kheireddine El-Boubbou ◽  
David C. Zhu ◽  
Xuefei Huang
Keyword(s):  
Hyaluronic Acid ◽  
Magnetic Nanoparticles ◽  
In Vivo Imaging ◽  
Cell Labelling ◽  
Simple Method ◽  
Highly Efficient

scholarly journals A New Dermal Substitute Containing Polyvinyl Alcohol with Silver Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 742
Author(s):  
Dario Mendes ◽  
Moema A. Hausen ◽  
Jéssica Asami ◽  
Akemi M. Higa ◽  
Fabio L. Leite ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
Inflammatory Responses ◽  
Fungal Growth ◽  
Collagen Matrix ◽  
Antibacterial Activities ◽  
Skin Substitute ◽  
Dermal Substitute

The experimental use of poly (alcohol-vinyl) (PVA) as a skin curative is increasing widely. However, the use of this hydrogel is challenging due to its favorable properties for microbiota growth. The association with silver nanoparticles (AgNPs) as an antimicrobial agent turns the match for PVA as a dressing, as it focuses on creating a physical barrier to avoid wound dehydration. When associated with extracellular components, such as the collagen matrix, the device obtained can create the desired biological conditions to act as a skin substitute. This study aimed to analyze the anti-microbiological activity and the in vitro and in vivo responses of a bilaminar device of PVA containing AgNPs associated with a membrane of collagen–hyaluronic acid (col-HA). Additionally, mesenchymal stem cells were cultured in the device to evaluate in vitro responses and in vivo immunomodulatory and healing behavior. The device morphology revealed a porous pattern that favored water retention and in vitro cell adhesion. Controlled wounds in the dorsal back of rat skins revealed a striking skin remodeling with new epidermis fulfilling all previously injured areas after 14 and 28 days. No infections or significant inflammations were observed, despite increased angiogenesis, and no fibrosis-markers were identified as compared to controls. Although few antibacterial activities were obtained, the addition of AgNPs prevented fungal growth. All results demonstrated that the combination of the components used here as a dermal device, chosen according to previous miscellany studies of low/mid-cost biomaterials, can promote skin protection avoiding infections and dehydration, minimize the typical wound inflammatory responses, and favor the cellular healing responses, features that give rise to further clinical trials of the device here developed

scholarly journals In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3070
Author(s):  
Hyeon Jin Ju ◽  
Mina Park ◽  
Ji Hoon Park ◽  
Gi Ru Shin ◽  
Hak Soo Choi ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Hyaluronic Acid ◽  
Residence Time ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tympanic Cavity ◽  
Nir Fluorescence ◽  
Degradation Pattern ◽  
Intratympanic Injection

In this study, we developed injectable intratympanic hyaluronic acid (HA) depots for the treatment of hearing loss. We prepared an injectable click-crosslinking formulation by modifying HA with tetrazine (HA-TET) and trans-cyclooctene (HA-TCO), which crosslinked to form an HA depot (Cx-HA). Preparation of the click-crosslinking HA formulation was facile, and Cx-HA depot formation was reproducible. Additionally, the Cx-HA hydrogel was significantly stiffer than HA hydrogel. To monitor the degradation pattern of hydrogels, we mixed a zwitterionic near-infrared (NIR) fluorophore (e.g., ZW800-1C) in the click-crosslinking HA formulation. Then, HA-TET and HA-TCO solutions containing ZW800-1C were loaded separately into the compartments of a dual-barrel syringe for intratympanic injection. The Cx-HA depots formed quickly, and an extended residence time in the tympanic cavity was confirmed by performing NIR fluorescence imaging. We have successfully prepared an injectable click-crosslinking HA formulation that has promise as an intratympanic drug depot.

Dynamic High-Resolution Ultrasound In Vivo Imaging of Hyaluronic Acid Filler Injection

2013 ◽  
Vol 39 (11) ◽  
pp. 1630-1636 ◽  
Author(s):  
Jocelyne C. Kohn ◽  
Alice S. Goh ◽  
Joseph L. Lin ◽  
Robert A. Goldberg
Keyword(s):  
Hyaluronic Acid ◽  
High Resolution ◽  
In Vivo Imaging ◽  
Filler Injection

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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