scholarly journals Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro Model Based on Human Primary Monocytes

2016 ◽  
Vol 8 (42) ◽  
pp. 28437-28447 ◽  
Author(s):  
Yang Li ◽  
Paola Italiani ◽  
Eudald Casals ◽  
Dirk Valkenborg ◽  
Inge Mertens ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Engineered Nanoparticles ◽  
Model Based ◽  
Vitro Model

scholarly journals 3D alveolar in vitro model based on epithelialized biomimetically curved culture membranes

Biomaterials ◽  
2021 ◽  
Vol 266 ◽  
pp. 120436
Author(s):  
D. Baptista ◽  
L. Moreira Teixeira ◽  
Z. Tahmasebi Birgani ◽  
S. van Riet ◽  
T. Pasman ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Model Based ◽  
Vitro Model

scholarly journals Optimization of an oral mucosa in vitro model based on cell line TR146

2020 ◽  
Vol 8 (2) ◽  
pp. 1748459 ◽  
Author(s):  
Grace C. Lin ◽  
Tamara Leitgeb ◽  
Alexandra Vladetic ◽  
Heinz-Peter Friedl ◽  
Nadine Rhodes ◽  
...  
Keyword(s):  
Cell Line ◽  
Oral Mucosa ◽  
In Vitro Model ◽  
Model Based ◽  
Vitro Model

An in vitro model based on cell monolayers grown on the underside of large- pore filters in bicameral chambers for studying thyrocyte-lymphocyte interactions

2004 ◽  
Vol 287 (6) ◽  
pp. C1763-C1768 ◽  
Author(s):  
Valérie Estienne ◽  
Nadège Brisbarre ◽  
Stéphanie Blanchin ◽  
Josée-Martine Durand-Gorde ◽  
Pierre Carayon ◽  
...  
Keyword(s):  
Antigen Processing ◽  
In Vitro Model ◽  
Thyroid Stimulating Hormone ◽  
Receptor Expression ◽  
Inverted Position ◽  
Model Based ◽  
Junction Protein ◽  
Vitro Model ◽  
Stimulating Hormone

In the processes underlying thyroid autoimmunity, thyrocytes probably act as antigen-presenting cells exposing T-cell epitopes to intrathyroid lymphocytes. To study the interactions between lymphocytes and thyrocytes, which are arranged in a tight, polarized monolayer, we developed a new in vitro model based on human thyrocytes grown on the underside of a filter placed in a bicameral chamber. Thyrocytes from Graves' disease glands were plated onto the upper face of a 8-μm-pore polyethylene terephthalate culture insert filter placed in the inverted position and grown for 24 h before the insert was returned to the normal position for a week in the cell culture plate wells. Thyrocytes grown in the presence of thyroid stimulating hormone, forming a homogeneous monolayer on the underside of the filter, reached confluence after 8 days in vitro. The cells developed a transepithelial electrical resistance >1,000 Ω·cm2, and the ZO-1 tight junction protein showed a junctional pattern of distribution. Thyrocytes showed a polarized pattern of thyroperoxidase and thyroid stimulating hormone receptor expression in the apical and basolateral positions, respectively. They were also found to aberrantly express DR class II human leukocyte antigen and an Fc immunoglobulin receptor (FcγRIIB2) in the basolateral and apical positions, respectively. Autologous intrathyroidal T lymphocytes cocultured for 24 h across the filter with the thyrocyte monolayer proliferated and remained in the upper chamber without any leakage occurring through the epithelial barrier, which makes this model particularly suitable for studying the cell-cell interactions involved in antigen processing.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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