Biointerface by Cell Growth on Graphene Oxide Doped Bacterial Cellulose/Poly(3,4-ethylenedioxythiophene) Nanofibers

2016 ◽  
Vol 8 (16) ◽  
pp. 10183-10192 ◽  
Author(s):  
Chuntao Chen ◽  
Ting Zhang ◽  
Qi Zhang ◽  
Xiao Chen ◽  
Chunlin Zhu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cell Growth ◽  
Bacterial Cellulose

scholarly journals Bacterial Cellulose as a Substrate for Microbial Cell Culture

2014 ◽  
Vol 80 (6) ◽  
pp. 1926-1932 ◽  
Author(s):  
Na Yin ◽  
Thiago M. A. Santos ◽  
George K. Auer ◽  
John A. Crooks ◽  
Piercen M. Oliver ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Physicochemical Properties ◽  
Stationary Phase ◽  
Bacterial Cellulose ◽  
Growth Rates ◽  
Bacterial Cell ◽  
Acetobacter Xylinum ◽  
Primary Factor ◽  
Nutrient Diffusion

ABSTRACTBacterial cellulose (BC) has a range of structural and physicochemical properties that make it a particularly useful material for the culture of bacteria. We studied the growth of 14 genera of bacteria on BC substrates produced byAcetobacter xylinumand compared the results to growth on the commercially available biopolymers agar, gellan, and xanthan. We demonstrate that BC produces rates of bacterial cell growth that typically exceed those on the commercial biopolymers and yields cultures with higher titers of cells at stationary phase. The morphology of the cells did not change during growth on BC. The rates of nutrient diffusion in BC being higher than those in other biopolymers is likely a primary factor that leads to higher growth rates. Collectively, our results suggest that the use of BC may open new avenues in microbiology by facilitating bacterial cell culture and isolation.

Improvement of antimicrobial activity of graphene oxide/bacterial cellulose nanocomposites through the electrostatic modification

2016 ◽  
Vol 136 ◽  
pp. 1152-1160 ◽  
Author(s):  
Xiao-Ning Yang ◽  
Dong-Dong Xue ◽  
Jia-Ying Li ◽  
Miao Liu ◽  
Shi-Ru Jia ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Graphene Oxide ◽  
Bacterial Cellulose ◽  
Cellulose Nanocomposites

scholarly journals Tunable stiffness of graphene oxide/polyacrylamide composite scaffolds regulates cytoskeleton assembly

2018 ◽  
Vol 9 (31) ◽  
pp. 6516-6522 ◽  
Author(s):  
Yupeng Sun ◽  
Kaixiang Zhang ◽  
Ruijie Deng ◽  
Xiaojun Ren ◽  
Can Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Graphene Oxide ◽  
Cell Growth ◽  
Specific Gene ◽  
Composite Scaffolds ◽  
Specific Gene Expression ◽  
Tunable Stiffness

Graphene oxide/polyacrylamide composite scaffolds with tunable stiffness are designed and fabricated to investigate the effect of extracellular matrix (ECM) stiffness on cytoskeleton assembly and specific gene expression during cell growth.

scholarly journals Graphene and its derivatives as biomedical materials: future prospects and challenges

2018 ◽  
Vol 8 (3) ◽  
pp. 20170056 ◽  
Author(s):  
Arghya Narayan Banerjee
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Graphene Oxide ◽  
Cell Growth ◽  
Drug Loading ◽  
Chemical Properties ◽  
Culture Cell ◽  
Biomedical Materials ◽  
Physico Chemical ◽  
Bio Imaging

Graphene and its derivatives possess some intriguing properties, which generates tremendous interests in various fields, including biomedicine. The biomedical applications of graphene-based nanomaterials have attracted great interests over the last decade, and several groups have started working on this field around the globe. Because of the excellent biocompatibility, solubility and selectivity, graphene and its derivatives have shown great potential as biosensing and bio-imaging materials. Also, due to some unique physico-chemical properties of graphene and its derivatives, such as large surface area, high purity, good bio-functionalizability, easy solubility, high drug loading capacity, capability of easy cell membrane penetration, etc., graphene-based nanomaterials become promising candidates for bio-delivery carriers. Besides, graphene and its derivatives have also shown interesting applications in the fields of cell-culture, cell-growth and tissue engineering. In this article, a comprehensive review on the applications of graphene and its derivatives as biomedical materials has been presented. The unique properties of graphene and its derivatives (such as graphene oxide, reduced graphene oxide, graphane, graphone, graphyne, graphdiyne, fluorographene and their doped versions) have been discussed, followed by discussions on the recent efforts on the applications of graphene and its derivatives in biosensing, bio-imaging, drug delivery and therapy, cell culture, tissue engineering and cell growth. Also, the challenges involved in the use of graphene and its derivatives as biomedical materials are discussed briefly, followed by the future perspectives of the use of graphene-based nanomaterials in bio-applications. The review will provide an outlook to the applications of graphene and its derivatives, and may open up new horizons to inspire broader interests across various disciplines.

One-Step In Situ Biosynthesis of Graphene Oxide-Bacterial Cellulose Nanocomposite Hydrogels

2014 ◽  
Vol 35 (19) ◽  
pp. 1706-1711 ◽  
Author(s):  
Hongjuan Si ◽  
Honglin Luo ◽  
Guangyao Xiong ◽  
Zhiwei Yang ◽  
Sudha R. Raman ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bacterial Cellulose ◽  
Nanocomposite Hydrogels ◽  
One Step

Enhanced conductivity of bacterial cellulose films reinforced with NH4I-doped graphene oxide

2019 ◽  
Vol 58 (14) ◽  
pp. 1585-1595 ◽  
Author(s):  
Fernando G. Torres ◽  
Robert Ccorahua ◽  
Junior Arroyo ◽  
Omar P. Troncoso
Keyword(s):  
Graphene Oxide ◽  
Bacterial Cellulose ◽  
Cellulose Films ◽  
Doped Graphene ◽  
Enhanced Conductivity

scholarly journals Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes

2019 ◽  
Vol 9 (2) ◽  
pp. 326 ◽  
Author(s):  
Somasekhar Chinnadayyala ◽  
Jinsoo Park ◽  
Yonghyun Choi ◽  
Jae-Hee Han ◽  
Ajay Yagati ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Cell Growth ◽  
Reduced Graphene Oxide ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Electrochemical Impedance ◽  
Hek293 Cells ◽  
Ito Electrode ◽  
Reduced Graphene

The improved binding ability of graphene–nanoparticle composites to proteins or molecules can be utilized to develop new cell-based assays. In this study, we fabricated reduced graphene oxide–gold nanoparticles (rGO-AuNP) electrodeposited onto a transparent indium tin oxide (ITO) electrode and investigated the feasibility of the electrochemical impedance monitoring of cell growth. The electrodeposition of rGO–AuNP on the ITO was optically and electrochemically characterized in comparison to bare, rGO-, and AuNP-deposited electrodes. The cell growth on the rGO–AuNP/ITO electrode was analyzed via electrochemical impedance measurement together with the microscopic observation of HEK293 cells transfected with a green fluorescent protein expression vector. The results showed that rGO–AuNP was biocompatible and induced an increase in cell adherence to the electrode when compared to the bare, AuNP-, or rGO-deposited ITO electrode. At 54 h cultivation, the average and standard deviation of the saturated normalized impedance magnitude of the rGO–AuNP/ITO electrode was 3.44 ± 0.16, while the value of the bare, AuNP-, and rGO-deposited ITO electrode was 2.48 ± 0.15, 2.61 ± 0.18, and 3.01 ± 0.25, respectively. The higher saturated value of the cell impedance indicates that the impedimetric cell-based assay has a broader measurement range. Thus, the rGO–AuNP/ITO electrode can be utilized for label-free and real-time impedimetric cell-based assays with wider dynamic range.

Sign in / Sign up

Export Citation Format

Share Document