Combined Effect of Cryogel Matrix and Temperature-Reversible Soluble–Insoluble Polymer for the Development of in Vitro Human Liver Tissue

2015 ◽  
Vol 8 (1) ◽  
pp. 264-277 ◽  
Author(s):  
Jyoti Kumari ◽  
Anjali A. Karande ◽  
Ashok Kumar
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Combined Effect ◽  
Human Liver Tissue ◽  
Insoluble Polymer

Characterization of in vitro healthy and pathological human liver tissue periodicity using backscattered ultrasound signals

2006 ◽  
Vol 32 (5) ◽  
pp. 649-657 ◽  
Author(s):  
Christiano Bittencourt Machado ◽  
Wagner Coelho de Albuquerque Pereira ◽  
Mahmoud Meziri ◽  
Pascal Laugier
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Human Liver Tissue ◽  
Ultrasound Signals

Liver slices in in vitro pharmacotoxicology with special reference to the use of human liver tissue

1997 ◽  
Vol 12 (1) ◽  
pp. 77-100 ◽  
Author(s):  
P. Olinga ◽  
D.K.F. Meijer ◽  
M.J.H. Slooff ◽  
G.M.M. Groothuis
Keyword(s):  
Special Reference ◽  
Liver Tissue ◽  
Human Liver ◽  
Human Liver Tissue ◽  
Liver Slices

scholarly journals Comparing in vitro human liver models to in vivo human liver using RNA-Seq

2020 ◽  
Author(s):  
Rajinder Gupta ◽  
Yannick Schrooders ◽  
Duncan Hauser ◽  
Marcel van Herwijnen ◽  
Wiebke Albrecht ◽  
...  
Keyword(s):  
Liver Cell ◽  
Liver Tissue ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Rna Seq ◽  
Cell Models ◽  
Human Liver Tissue ◽  
Human Liver Cell

Abstract The liver plays an important role in xenobiotic metabolism and represents a primary target for toxic substances. Many different in vitro cell models have been developed in the past decades. In this study, we used RNA-sequencing (RNA-Seq) to analyze the following human in vitro liver cell models in comparison to human liver tissue: cancer-derived cell lines (HepG2, HepaRG 3D), induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs), cancerous human liver-derived assays (hPCLiS, human precision cut liver slices), non-cancerous human liver-derived assays (PHH, primary human hepatocytes) and 3D liver microtissues. First, using CellNet, we analyzed whether these liver in vitro cell models were indeed classified as liver, based on their baseline expression profile and gene regulatory networks (GRN). More comprehensive analyses using non-differentially expressed genes (non-DEGs) and differential transcript usage (DTU) were applied to assess the coverage for important liver pathways. Through different analyses, we noticed that 3D liver microtissues exhibited a high similarity with in vivo liver, in terms of CellNet (C/T score: 0.98), non-DEGs (10,363) and pathway coverage (highest for 19 out of 20 liver specific pathways shown) at the beginning of the incubation period (0 h) followed by a decrease during long-term incubation for 168 and 336 h. PHH also showed a high degree of similarity with human liver tissue and allowed stable conditions for a short-term cultivation period of 24 h. Using the same metrics, HepG2 cells illustrated the lowest similarity (C/T: 0.51, non-DEGs: 5623, and pathways coverage: least for 7 out of 20) with human liver tissue. The HepG2 are widely used in hepatotoxicity studies, however, due to their lower similarity, they should be used with caution. HepaRG models, iPSC-HLCs, and hPCLiS ranged clearly behind microtissues and PHH but showed higher similarity to human liver tissue than HepG2 cells. In conclusion, this study offers a resource of RNA-Seq data of several biological replicates of human liver cell models in vitro compared to human liver tissue.

Reactivity of atropaldehyde, a felbamate metabolite in human liver tissue in vitro

2002 ◽  
Vol 142 (1-2) ◽  
pp. 119-134 ◽  
Author(s):  
Izet M Kapetanovic ◽  
Cynthia D Torchin ◽  
John M Strong ◽  
Wayne D Yonekawa ◽  
Chuang Lu ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Human Liver Tissue

scholarly journals IN VITRO ENGINEERING OF A FUNCTIONAL HUMAN LIVER TISSUE FOR TRANSPLANTATION

2010 ◽  
Vol 90 ◽  
pp. 464
Author(s):  
J. Bierwolf ◽  
M. Luetgehetmann ◽  
S. Cohen ◽  
O. Kryukov ◽  
S. Deichmann ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Human Liver Tissue
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